Differential pH measurements of metabolic cellular activity in nl culture volumes using microfabricated iridium oxide electrodes

Ges, Igor A.; Ivanov, B.; Werdich, Andreas A.; Baudenbacher, Franz

doi:10.1016/j.bios.2006.05.033

Cited by 63 publications

(54 citation statements)

References 27 publications

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“…This cyclic voltammetric behavior is well in accord with those of previously reported IrO x electrodes that allowed stable electrode potentials in buffered solutions [31]. Generally, other methods for preparing IrO x electrodes require prolonged repeated potential cycling (and a complicated procedure for preparing iridium ion solutions) [15][16][17][18]. In the case of IrO x nanoparticle-based deposition, IrO x nanoparticles that possess the characteristics required for a pseudoreference electrode were presynthesized in large quantities, and were then deposited on a specific electrode in a short time.…”

Section: Performance Of Iro X Nanoparticle-based Pseudoreference Elecsupporting

confidence: 85%

Immunosensing Microchip Using Fast and Selective Preparation of an Iridium Oxide Nanoparticle‐Based Pseudoreference Electrode

Aziz

Kim

et al. 2011

Electroanalysis

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Iridium oxide (IrO x ) electrodes have been used as pseudoreference electrodes operating in buffered solutions, but their preparation requires a time-consuming procedure. Here we report a simple, fast, and selective preparation of an IrO x pseudoreference electrode based on the deposition of presynthesized IrO x nanoparticles. The reference electrode is applied to an immunosensing microchip consisting of a patterned poly(dimethylsiloxane) plate and an indium-tin oxide-micropatterned glass. The sequence of microfabrication is optimized to accomplish better performance. The microchip is operated by capillary-driven microfluidic controls and used for detecting a target mouse IgG. The measured detection limit in the microchip is ca. 10 pg/mL.

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Section: Performance Of Iro X Nanoparticle-based Pseudoreference Elecsupporting

confidence: 85%

Immunosensing Microchip Using Fast and Selective Preparation of an Iridium Oxide Nanoparticle‐Based Pseudoreference Electrode

Aziz

Kim

et al. 2011

Electroanalysis

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“…Poly (amic acid, Sigma-Aldrich Co.) ink was used as the waterproof layer printed on the top layer of MEAs and was prepared by diluting 10% (m/m) poly (amic acid) solution in highly pure N-methyl-2-pyrrolidone (NMP) to 1% (m/m) [22]. Iridium (IV) chloride, hydrogen peroxide and potassium oxalate (Fisher Scientific Co.) were prepared for iridium tetrachloride solution for pH working electrode [24,27,28]. Phosphate-buffered saline (PBS) solution was prepared by mixing sodium hydrogen phosphate, sodium chloride, potassium chloride and potassium dihydrogen phosphate (Fisher Scientific Co.) in the DI water, and was used for the stabilizing solution for the pH working electrodes.…”

Section: Sensor Materials Preparationmentioning

confidence: 99%

Real-time in situ sensing of multiple water quality related parameters using micro-electrode array (MEA) fabricated by inkjet-printing technology (IPT)

Dong

Otieno

et al. 2016

Sensors and Actuators B: Chemical

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“…A detailed protocol to deposit the iridium oxide films onto the Pt-films and their properties was described in previous work (Ges et al, 2005(Ges et al, , 2007. The deposition solution was prepared based on the method proposed by Yamanaka (1989Yamanaka ( , 1991 and Marzouk et al (1998;Marzouk, 2003).…”

Section: Iro X Ph Sensitivity and Reference Electrodesmentioning

confidence: 99%

“…In our previous work (Ges et al, 2007), we described a new approach to measure pH differences in microfluidic devices. We used two IrO x thin film electrodes: one to sense the pH and the other as quasi-reference.…”

Section: Characterization Of Ph Microsensormentioning

confidence: 99%

On-chip acidification rate measurements from single cardiac cells confined in sub-nanoliter volumes

Ges

Dzhura

Baudenbacher

2008

Biomed Microdevices

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The metabolic activity of cells can be monitored by measuring the pH in the extracellular environment. Microfabrication and microfluidic technologies allow the sensor size and the extracellular volumes to be comparable to single cells. A glass substrate with thin film pH sensitive IrO x electrodes was sealed to a replica-molded polydimethylsiloxane (PDMS) microfluidic network with integrated valves. The device, termed NanoPhysiometer, allows the trapping of single cardiac myocytes and the measurement of the pH in a detection volume of 0.36 nL. For wild-type (WT) single cardiac myocytes an acidification rate of 6.45 ± 0.38 mpH/min was measured in comparison to 19.5 ± 0.38 mpH/min for very long chain Acyl-CoA dehydrogenase (VLCAD) deficient mice in 0.8 mM of Ca 2+ . VLCAD deficiency is a fatty acid oxidation disease leading to cardiomyopathy and arrhythmias. The acidification rate increased to 11.96 ± 1.33 mpH/min for WT and to 32.0 ± 4.64 mpH/min for VLCAD −/− in 1.8 mM of Ca 2+ . The NanoPhysiometer concept can be extended to study ischemia/reperfusion injury or disorders of other biological systems to identify strategies for treatment and possible pharmacological targets.

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Differential pH measurements of metabolic cellular activity in nl culture volumes using microfabricated iridium oxide electrodes

Cited by 63 publications

References 27 publications

Immunosensing Microchip Using Fast and Selective Preparation of an Iridium Oxide Nanoparticle‐Based Pseudoreference Electrode

Immunosensing Microchip Using Fast and Selective Preparation of an Iridium Oxide Nanoparticle‐Based Pseudoreference Electrode

Real-time in situ sensing of multiple water quality related parameters using micro-electrode array (MEA) fabricated by inkjet-printing technology (IPT)

On-chip acidification rate measurements from single cardiac cells confined in sub-nanoliter volumes

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