Differential phytotoxic effect of silver nitrate (AgNO3) and bifunctionalized silver nanoparticles (AgNPs-Cit-L-Cys) on Lemna plants (duckweeds)

Iannelli, Maria Adelaide; Bellini, A.; Venditti, Iole; Casentini, B.; Battocchio,; Scalici, Massimiliano; Ceschin, Simona

doi:10.1016/j.aquatox.2022.106260

Cited by 16 publications

(11 citation statements)

References 45 publications

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“…The biomarker of oxidative damage to proteins, expressed as protein carbonyls (C=O), only increased in the duckweed treated with higher concentrations (2 and 5 mg/L) of AgNPs whereas lower concentrations of nanoparticles had no effect on that parameter ( Figure 2 B). Similar to this study, several other reports have confirmed a significant increase in ROS, MDA and protein carbonylation in different plant species following treatments with AgNPs [ 4 , 19 , 21 ].…”

Section: Resultssupporting

confidence: 92%

“…Lower concentrations of AgNPs did not affect the growth whilst higher concentrations (2 and 5 mg/L) caused a significant reduction in the duckweed growth rate ( Figure 1 B). A reduction in growth due to AgNPs of different sizes and concentrations has been reported in aquatic and terrestrial plants [ 3 , 8 , 9 , 15 , 21 , 22 , 23 , 24 ]. The decreased growth rate of duckweed after the AgNP treatment may have been connected to the accumulation of Ag in the plant cells and consequent changes in the nutrient contents ( Table 1 ).…”

Section: Resultsmentioning

confidence: 99%

“…A decrease in the level of photosynthetic pigments after exposure to AgNPs has been recorded in mustard seedlings [ 9 ], duckweed species [ 17 , 19 , 21 ] and in tobacco [ 27 ]. Evidence shows that heavy metals, in general, negatively affect chlorophylls synthesis by a substitution of Mg 2+ with heavy metal ions in the chlorophyll molecule, by chloroplast membrane degradation due to lipid peroxidation or by a reduced antioxidant concentration [ 28 ].…”

Section: Resultsmentioning

confidence: 99%

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Phytotoxic Action of Silver Nanoparticles on Lemna minor: Multi-Parameter Analysis of Different Physiological Processes

Ljubimir

Domijan

Brkanac

2023

Plants

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Considering the widespread use of silver nanoparticles (AgNPs) and their consequent build-up in waterways, there is a concern about the hazardous effect of AgNPs for aquatic ecosystems. The aim of this study was to clarify the mechanism of the action of AgNPs on duckweed (Lemna minor L.) by evaluating multiple parameters in different physiological processes. Duckweed was treated with AgNPs in a concentration range of 0.5 to 5 mg/L over a 7-day period. The analysis revealed that the AgNP-treated duckweed accumulated Ag in accordance with increasing AgNP concentrations. Furthermore, higher concentrations (2 and 5 mg/L) of AgNPs negatively affected N, P and especially K and Mg levels in the plant tissue. Accordingly, the plant growth and photosynthetic parameters were more inhibited in response to higher concentrations of AgNPs. Nanosilver significantly increased the generation of ROS at higher concentrations, although lipid peroxidation was significant even at the lowest concentration of AgNPs. However, defense mechanisms were able to counteract AgNP-induced oxidative stress and balance the intracellular redox status, as evidenced by increased activities of the main detoxification enzymes. With this experimental setting, AgNPs exhibited a relatively weak phytotoxicity at 0.5 and 1 mg/L; nevertheless, silver in a nano form poses a hazard for plants, considering its continuous release into aquatic environments.

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Section: Resultssupporting

confidence: 92%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Phytotoxic Action of Silver Nanoparticles on Lemna minor: Multi-Parameter Analysis of Different Physiological Processes

Ljubimir

Domijan

Brkanac

2023

Plants

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“…In addition, changes in the activity of antioxidant enzymes are characteristic when these plants have disturbed homeostasis. They respond to glyphosate by increasing peroxidase activity [ 57 ], and exposure to silver nitrate corresponds to an increase in catalase activity and content of malondialdehyde, and these answers and morphological parameters may be different for Lemna minor and Lemna minuta [ 58 ].…”

Section: Discussionmentioning

confidence: 99%

The Effect of Quinolones on Common Duckweed Lemna minor L., a Hydrophyte Bioindicator of Environmental Pollution

Sikorski

Bęś

Warmiński

2023

IJERPH

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Plant growth and the development of morphological traits in plants are inhibited under exposure to pharmaceuticals that are present in soil and water. The present study revealed that moxifloxacin (MOXI), nalidixic acid (NAL), levofloxacin (LVF) and pefloxacin (PEF) at concentrations of >0.29, >0.48, >0.62 and >1.45 mg × L−1, respectively, inhibited the growth (Ir) of duckweed plants and decreased their yield (Iy). In the current study, none of the tested quinolones (QNs) at any of the examined concentrations were lethal for common duckweed plants. However, at the highest concentration (12.8 mg × L−1), LVF increased Ir and Iy values by 82% on average and increased the values of NAL, PEF and MOXI by 62% on average. All tested QNs led to the loss of assimilation pigments. In consequence, all QNs, except for LVF, induced changes in chlorophyll fluorescence (Fv/Fm), without any effect on phaeophytinization quotient (PQ) values. The uptake of NAL, MOXI, LVF by Lemna minor during the 7-day chronic toxicity test was directly proportional to drug concentrations in the growth medium. Nalidixic acid was absorbed in the largest quantities, whereas in the group of fluoroquinolones (FQNs), MOXI, LVF and PEF were less effectively absorbed by common duckweed. This study demonstrated that biosorption by L. minor occurs regardless of the plants’ condition. These findings indicate that L. minor can be used as an effective biological method to remove QNs from wastewater and water and that biosorption should be a mandatory process in conventional water and wastewater treatment.

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“…Moreover, due to axenic and controlled conditions (light, temperature, humidity, and composition of nutrient media), plant cell culture reduces environmental variations that can affect the bioavailability of the toxic substances in the culture medium (Iori et al 2012a ; Ghorbanpour et al 2018 ). Therefore, in the present study, we investigated the potential chronic toxicity of Ag in the form of bifunctionalized silver nanoparticles (AgNPs-Cit-L-Cys), novel nanomaterials intended for environmental applications in pollution monitoring and remediation (Schiesaro et al 2020 ; Bellingeri et al 2022 ; Iannelli et al 2022 ), and silver nitrate (AgNO 3 ) on callus culture of Populus nigra L. (clone Poli), a model plant used commonly in the research of abiotic stress, whose capability to tolerate environmental pollutants was previously assessed in in vitro approaches (Iori et al 2012a , b ).…”

Section: Introductionmentioning

confidence: 99%

Phytotoxic impact of bifunctionalized silver nanoparticles (AgNPs-Cit-L-Cys) and silver nitrate (AgNO3) on chronically exposed callus cultures of Populus nigra L.

Iori,

Muzzini,

Venditti

et al. 2023

Environ Sci Pollut Res

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Owing to the unique physicochemical properties and the low manufacturing costs, silver nanoparticles (AgNPs) have gained growing interest and their application has expanded considerably in industrial and agricultural sectors. The large-scale production of these nanoparticles inevitably entails their direct or indirect release into the environment, raising some concerns about their hazardous aspects. Callus culture represents an important tool in toxicological studies to evaluate the impact of nanomaterials on plants and their potential environmental risk. In this study, we investigated the chronic phytotoxic effects of different concentrations of novel bifunctionalized silver nanoparticles (AgNPs-Cit-L-Cys) and silver nitrate (AgNO3) on callus culture of Populus nigra L., a pioneer tree species in the riparian ecosystem. Our results showed that AgNPs-Cit-L-Cys were more toxic on poplar calli compared to AgNO3, especially at low concentration (2.5 mg/L), leading to a significant reduction in biomass production, accompanied by a decrease in protein content, a significant increase in both lipid peroxidation level, ascorbate peroxidase (APX), and catalase (CAT) enzymatic activities. In addition, these findings suggested that the harmful activity of AgNPs-Cit-L-Cys might be correlated with their physicochemical properties and not solely attributed to the released Ag+ ions and confirmed that AgNPs-Cit-L-Cys phytoxicity is associated to oxidative stress.

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Differential phytotoxic effect of silver nitrate (AgNO3) and bifunctionalized silver nanoparticles (AgNPs-Cit-L-Cys) on Lemna plants (duckweeds)

Cited by 16 publications

References 45 publications

Phytotoxic Action of Silver Nanoparticles on Lemna minor: Multi-Parameter Analysis of Different Physiological Processes

Phytotoxic Action of Silver Nanoparticles on Lemna minor: Multi-Parameter Analysis of Different Physiological Processes

The Effect of Quinolones on Common Duckweed Lemna minor L., a Hydrophyte Bioindicator of Environmental Pollution

Phytotoxic impact of bifunctionalized silver nanoparticles (AgNPs-Cit-L-Cys) and silver nitrate (AgNO3) on chronically exposed callus cultures of Populus nigra L.

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