Differential protein expression in ovaries of uninfected and Babesia-infected southern cattle ticks, Rhipicephalus (Boophilus) microplus

Rachinsky, Anna; Guerrero, Felix D.; Scoles, Glen A.

doi:10.1016/j.ibmb.2007.08.001

Cited by 59 publications

(68 citation statements)

References 72 publications

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“…In fact, the observation of a Nonalaris transcript within ticks (TC4684) is the first to our knowledge. The Heptalaris transcript present in this study (TC1750) had previously been described by Rachinsky et al (2007) (identified as TC9311) who suggested that due to variable splicing it could give rise to several shorter Kunitz domain-containing proteins. Whether this is also the case for TC4684, a close homologue of TC1750, should be confirmed through additional proteomic studies.…”

Section: Discussionmentioning

confidence: 66%

Evolution of the tissue factor pathway inhibitor-like Kunitz domain-containing protein family in Rhipicephalus microplus

Louw

Merwe

Neitz

et al. 2013

International Journal for Parasitology

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One of the principle mechanisms utilised by ticks to obtain a blood meal is the subversion of the host's haemostatic response. This is achieved through the secretion of saliva containing antihaemostatic proteins into the feeding lesion. Lineage-specific expansion of predicted secretory protein families have been observed in all previously studied ticks and occurred in response to adaptation to a blood-feeding environment. Of these, the predominant families are common between both hard and soft ticks. One of these families, namely the Kunitz domain-containing protein family, includes proven tissue factor pathway inhibitor-like (TFPI-like) anti-haemostatics such as ixolaris and penthalaris that play a crucial role during tick feeding. Although Kunitz-type proteins have been found in Rhipicephalus microplus, the TFPI-like Kunitz protein family has not yet been studied. We report a comprehensive search for TFPI-like Kunitz domain-containing proteins in R. microplus expressed sequence tag libraries, resulting in the identification of 42 Page 2 of 36 homologues. The homologues were bioinformatically and phylogenetically studied, including the application of an intensive Bayesian Markov Chain Monte Carlo (MCMC) analysis of the individual Kunitz domain nucleotide sequences. We show that the R. microplus TFPI-like Kunitz protein family groups into two main clades that presumably underwent ancient duplication, which indicates that a whole genome duplication event occurred at least 150 million years ago. Evidence for recent and ancient gene and domain duplication events was also found. Furthermore, the divergence times of the various tick lineages estimated in this paper correspond with those presented in previous studies. The elucidation of this large protein family's evolution within R. microplus adds to current knowledge of this economically important tick.

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Section: Discussionmentioning

confidence: 66%

Evolution of the tissue factor pathway inhibitor-like Kunitz domain-containing protein family in Rhipicephalus microplus

Louw

Merwe

Neitz

et al. 2013

International Journal for Parasitology

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“…The limited number of tick sequences available in databases is a serious drawback to the identification of tick proteins using proteomic approaches. Rachinsky et al (2007) investigated the proteins that were differentially expressed in ovarian tissues collected from Babesia bovis-infected and uninfected R. microplus and could only identify 19 of their 43 differentially expressed proteins. Similarly, Oleaga et al (2007) could only identify 27 spots out a total of 97 analyzed in the salivary glands of O. moubata and O. erraticus.…”

Section: Comparative 2d-gel Analysis Of Hemolymph Proteinsmentioning

confidence: 99%

“…Since the first paper was published by Madden et al (2002) highlighting the complications associated with using proteomics approaches to characterize protein profiles of two related tick species, Amblyomma americanum and A. maculatum, several papers using the above approach have appeared in the tick field (Untalan et al, 2005;Vennestrom and Jensen, 2007;Oleaga et al, 2007;Francischetti et al, 2008aFrancischetti et al, , 2008bRachinsky et al, 2007Rachinsky et al, & 2008Wickramasekara et al, 2008). Most of these papers described the identification of either salivary gland or larval proteins from a few hard and soft tick species.…”

Section: Introductionmentioning

confidence: 99%

“…Most of these papers described the identification of either salivary gland or larval proteins from a few hard and soft tick species. Only two papers have reported using differential protein expression to identify proteins modulated in Babasia bovisinfected Rhipicephalus (Boophilus) microplus ovaries and midgut (Rachinsky et al, 2007(Rachinsky et al, , 2008.…”

Section: Introductionmentioning

confidence: 99%

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A proteomics approach for the analysis of hemolymph proteins involved in the immediate defense response of the soft tick, Ornithodoros savignyi, when challenged with Candida albicans

2010

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A proteomics approach was employed to identify proteins secreted into the hemolymph of O. savignyi ticks two hours after immune-challenge with the yeast, Candida albicans. Profiling of the proteins present in hemolymph of unchallenged ticks versus ticks challenged with heat-killed yeast revealed 5 proteins to be differentially expressed. The modulated protein spots were subjected to tandem mass spectrometry (MS/MS) analysis, but could not be positively identified. These proteins can be assigned to the immune response as they were not induced after aseptic injury. In an attempt to identify hemolymph proteins that recognize and bind to yeast cells, hemolymph obtained from both unchallenged and challenged ticks was incubated with C. albicans. Elution of the bound proteins followed by SDS-PAGE analysis indicated that three proteins (97, 88 and 26 kDa) present in both unchallenged and challenged hemolymph samples bind to yeast cells. The constant presence of these three proteins in tick hemolymph leads us to believe that they may be involved in non-self recognition and participate in yeast clearance from tick plasma. The analyzed yeast-binding proteins could also not be positively identified, suggesting that all the tick immune proteins investigated in this study are novel.

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“…A indução da síntese de uma família de inibidores Kunitz-type também foi observada em ovários de R. microplus após a infecção pelo protozoário Babesia bovis (Rachinsky et al, 2007). Além disso, foi relatado que o inibidor Kunitz-type de D. variabilis (DvKPI) apresenta uma atividade bacteriostática contra R. montanensis in vitro (Ceraul et al, 2008).…”

Section: Discussionunclassified

Efeitos da infecção por Rickettsia rickettsii sobre o perfil de expressão gênica do carrapato vetor Amblyomma cajennense.

Martins¹

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Differential protein expression in ovaries of uninfected and Babesia-infected southern cattle ticks, Rhipicephalus (Boophilus) microplus

Cited by 59 publications

References 72 publications

Evolution of the tissue factor pathway inhibitor-like Kunitz domain-containing protein family in Rhipicephalus microplus

Evolution of the tissue factor pathway inhibitor-like Kunitz domain-containing protein family in Rhipicephalus microplus

A proteomics approach for the analysis of hemolymph proteins involved in the immediate defense response of the soft tick, Ornithodoros savignyi, when challenged with Candida albicans

Efeitos da infecção por Rickettsia rickettsii sobre o perfil de expressão gênica do carrapato vetor Amblyomma cajennense.

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