Differential protein expression using proteomics from a crustacean brine shrimp ( Artemia sinica ) under CO 2 -driven seawater acidification

Chang, Xue-jiao; Zheng, Chao; Wang, Yuwei; Chen, Meng; Xie, Xiaolu; Liu, Haipeng

doi:10.1016/j.fsi.2016.10.008

Cited by 19 publications

(10 citation statements)

References 46 publications

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“…Prophenoloxidase participates in melanization, and its activation promotes phagocytosis, encapsulation, and nodule formation for the protection against invading pathogenic microorganisms (Vazquez et al, 2009). Two long-term studies (1 to 14 days) with brine shrimp (Artemia sinica) evidenced an increase in proPO gene expression during the seventh day post water acidification (Zheng et al, 2015;Chang et al, 2016). Although ours was a short-time study, in which proPO gene expression increased at 6 and 12 h but decreased at 24 h, we agree that non-lethal high CO 2 can affect pathogen recognition via proPO-activating system.…”

Section: Discussionmentioning

confidence: 99%

“…Our study demonstrated how non-lethal high CO 2 level influenced the transcriptional response of immune-related genes of L. vannamei. The gene expression modulation by water acidification promotes metabolic suppression, reduced protein synthesis and respiratory stress, and reduced metabolic scope, causing pathogen invasion, disease transmission, and host susceptibility (Chang et al, 2016;Wang et al, 2016). On the other hand, the energy destined to transcriptional response modulation can reduce shrimp muscular growth and productive performance (Silveira et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

“…Transcriptional response of immune-related genes in Litopenaeus vannamei cultured in recirculating... Hernández et al High CO 2 concentrations contribute to the system acidification (Skov, 2019), which can negatively affect growth, physiology, energy metabolism, and immunity of fish (Dennis III et al, 2015;Good et al, 2018;Khan et al, 2018;Almroth et al, 2019;Hermann et al, 2019;Mota et al, 2019;Machado et al, 2020;Pan et al, 2020;Mota et al, 2020), crustaceans (Fehsenfeld et al, 2011;Rathburn et al, 2013;Johnson et al, 2015;Zheng et al, 2015;Chang et al, 2016;Meseck et al, 2016), and mollusks (Bibby et al, 2008;Wang et al, 2016;Clements et al, 2021). High non-lethal (23.8 mg L −1 ), lethal (59.12 mg L −1 ), and safe (5.9 mg L −1 ) CO 2 levels for L. vannamei production in RAS systems were determined (Furtado et al, 2017), but concentration above 20 mg L −1 reduces tissue oxygenation and increases the ventilation rate (Furtado et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

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Transcriptional response of immune-related genes in Litopenaeus vannamei cultured in recirculating aquaculture systems with elevated CO2

Casillas-Hernández¹,

Arévalo‐Sainz²,

Flores‐Pérez³

et al. 2021

Revista Brasileira De Zootecnia

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This short-term study evaluated the effect of non-lethal high CO 2 concentration on the transcriptional response of immune-related genes of Pacific white shrimp (Litopenaeus vannamei) cultured in recirculating aquaculture systems (RAS). Two experimental groups were created: high CO 2 (47.67±2.04 mg L −1 ) and low CO 2 (2.0±1.93 mg L −1 ). Shrimp of 8.85±1.20 g were placed randomly at a density equivalent to 100 individuals m −3 and were monitored at 6, 12, 18, and 24 h. The transcriptional response of immune-related genes was analyzed by qPCR. Gene expression of hemocyanin, prophenoloxidase, and heat shock protein 60 was downregulated at 24 h, suggesting affectations on oxygen transportation, melanization, and protein functioning of L. vannamei under high CO 2 concentrations. Also, gene up-regulation of lipopolysaccharide-and β-glucan-binding protein and cytosolic manganese superoxide dismutase can impair the bacterial recognition and antioxidant defense of shrimp exposed to high CO 2 concentrations. These results suggest that concentration at about 47 mg L −1 of CO 2 can significantly influence the transcriptional response modulation of immune-related genes.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Transcriptional response of immune-related genes in Litopenaeus vannamei cultured in recirculating aquaculture systems with elevated CO2

Casillas-Hernández¹,

Arévalo‐Sainz²,

Flores‐Pérez³

et al. 2021

Revista Brasileira De Zootecnia

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“…Furthermore, although GAPDH is a key glycolytic enzyme, studies from prokaryotes to vertebrates have shown that it has multiple roles unrelated to its enzyme activity in glycolysis in the cells, and thus, it is considered a "moonlighting protein" (White and Garcin, 2017). In crustaceans, GAPDH gene expression and protein activity are sensitive to several stressors such as diseases (Liu et al, 2011;Wang et al, 2007), high hydrostatic pressure (Morris et al, 2015), hydrocarbons (Pasquevich et al, 2013), water acidification (Chang et al, 2016;Hauton et al, 2009), cold stress (Fan et al, 2013), hypercapnic hypoxia (Johnson et al, 2015) and hypoxia (Camacho-Jiménez et al, 2018). Although most of the knowledge on GAPDH molecular biology in animal models can be translated from the findings in vertebrates, the specific mechanisms underlying the transcriptional control of GAPDH are poorly understood in crustaceans as well as in most aquatic invertebrates.…”

Section: Discussionmentioning

confidence: 99%

Regulation of glyceraldehyde-3-phosphate dehydrogenase by hypoxia inducible factor 1 in the white shrimp Litopenaeus vannamei during hypoxia and reoxygenation

Camacho‐Jiménez

Leyva‐Carrillo

Peregrino‐Uriarte

et al. 2019

Comparative Biochemistry and Physiology Part A: Molecular &

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Hypoxia is a frequent source of stress in the estuarine habitat of the white shrimp Litopenaeus vannamei. During hypoxia, L. vannamei gill cells rely more heavily on anaerobic glycolysis to obtain ATP. This is mediated by transcriptional up-regulation of glycolytic enzymes including glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The hypoxia inducible factor 1 (HIF-1) is an important transcriptional activator of several glycolytic enzymes during hypoxia in diverse animals, including crustaceans. In this work, we cloned and sequenced a fragment corresponding to the 5′ flank of the GAPDH gene and identified a putative HIF-1 binding site, as well as sites for other transcription factors involved in the hypoxia signaling pathway. To investigate the role of HIF-1 in GAPDH regulation, we simultaneously injected double-stranded RNA (dsRNA) into shrimp to silence HIF-1α and HIF-1β under normoxia, hypoxia, and hypoxia followed by reoxygenation, and then measured gill HIF-1α, HIF-1β expression, GAPDH expression and activity, and glucose and lactate concentrations at 0, 3, 24 and 48 h. During normoxia, HIF-1 silencing induced up-regulation of GAPDH transcripts and activity, suggesting that expression is down-regulated via HIF-1 under these conditions. In contrast, HIF-1 silencing during hypoxia abolished the increases in GAPDH expression and activity, glucose and lactate concentrations. Finally, HIF-1 silencing during hypoxia-reoxygenation prevented the increase in GAPDH expression, however, those changes were not reflected in GAPDH activity and lactate accumulation. Altogether, these results indicate that GAPDH and glycolysis are transcriptionally regulated by HIF-1 in gills of white shrimp.

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“…The balance of acid-alkali ions in water is of essential importance for aquatic crustaceans [42–46]. The decreasing pH value in seawaters caused by ocean acidification (OA) [47] negatively affects the mortality, growth, and reproduction of marine crustaceans [48–50]. Kurihara et al (2008) evaluated that the survival rates of Palaemon pacificus were largely reduced (55%) after long-term (30 weeks) exposing to low-pH environment (pH 7.89 ± 0.05) [51].…”

Section: Introductionmentioning

confidence: 99%

Analysis of the transcriptome data in Litopenaeus vannamei reveals the immune basis and predicts the hub regulation-genes in response to high-pH stress

Huang

Cheng

et al. 2018

PLoS ONE

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Soil salinization erodes the farmlands and poses a serious threat to human life, reuse of the saline-alkali lands as cultivated resources becomes increasingly prominent. Pacific white shrimp (Litopenaeus vannamei) is an important farmed aquatic species for the development and utilization of the saline-alkali areas. However, little is known about the adaptation mechanism of this species in terms of high-pH stress. In the present study, a transcriptome analysis on the gill tissues of L. vannamei in response to high-pH stress (pH 9.3 ± 0.1) was conducted. After analyzing, the cyclic nucleotide gated channel-Ca2+ (CNGC-Ca2+) and patched 1 (Ptc1) were detected as the majority annotated components in the cAMP signaling pathway (KO04024), indicating that the CNGC-Ca2+ and Ptc1 might be the candidate components for transducing and maintaining the high-pH stress signals, respectively. The immunoglobulin superfamily (IgSF), heat shock protein (HSP), glutathione s-transferase (GST), prophenoloxidase/phenoloxidase (proPO/PO), superoxide dismutase (SOD), anti-lipopolysaccharide factor (ALF) and lipoprotein were discovered as the major transcribed immune factors in response to high-pH stress. To further detect hub regulation-genes, protein-protein interaction (PPI) networks were constructed; the genes/proteins “Polymerase (RNA) II (DNA directed) polypeptide A” (POLR2A), “Histone acetyltransferase p300” (EP300) and “Heat shock 70kDa protein 8” (HSPA8) were suggested as the top three hub regulation-genes in response to acute high-pH stress; the genes/proteins “Heat shock 70kDa protein 4” (HSPA4), “FBJ murine osteosarcoma viral oncogene homolog” (FOS) and “Nucleoporin 54kDa” (NUP54) were proposed as the top three hub regulation-genes involved in adapting endurance high-pH stress; the protein-interactions of “EP300-HSPA8” and “HSPA4-NUP54” were detected as the most important biological interactions in response to the high-pH stress; and the HSP70 family genes might play essential roles in the adaptation of the high-pH stress environment in L. vannamei. These findings provide the first insight into the molecular and immune basis of L. vannamei in terms of high-pH environments, and the construction of a PPI network might improve our understanding in revealing the hub regulation-genes in response to abiotic stress in shrimp species and might be beneficial for further studies.

show abstract

Differential protein expression using proteomics from a crustacean brine shrimp ( Artemia sinica ) under CO 2 -driven seawater acidification

Cited by 19 publications

References 46 publications

Transcriptional response of immune-related genes in Litopenaeus vannamei cultured in recirculating aquaculture systems with elevated CO2

Transcriptional response of immune-related genes in Litopenaeus vannamei cultured in recirculating aquaculture systems with elevated CO2

Regulation of glyceraldehyde-3-phosphate dehydrogenase by hypoxia inducible factor 1 in the white shrimp Litopenaeus vannamei during hypoxia and reoxygenation

Analysis of the transcriptome data in Litopenaeus vannamei reveals the immune basis and predicts the hub regulation-genes in response to high-pH stress

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