Differential pulse voltammetric determination of dopamine with the coexistence of ascorbic acid on boron-doped diamond surface

Abstract

Electrochemical determination of dopamine (DA) in the presence of ascorbic acid (AA) was achieved on boron-doped diamond (BDD) film electrode by differential pulse voltammetry. The experimental results indicated that the oxidative peaks of DA and AA could be separated completely on anodically-treated (BDD) electrode without further modification, although these two peaks can not be separated on glassy carbon electrode. The peak separation of DA and AA was developed to be 0.44 V. High sensitivity was obtained to determine DA selectively with the coexisting of a large excess of AA in acidic media by DPV. The detection limit of DA was achieved to be 1.1 × 10 −6 M in the presence of AA with the concentration of 200 times more than DA. This technique was also applied to the determination of DA in real samples.

Preparation and Study of Colloidal CdO Nanoparticles by Laser Ablation in Polyvinylpyrrolidone

“…It can be seen that the value of the energy gap is about 2.8 eV. This optical phenomenon indicates that these nanoparticles show the quantum size effect [15,16]. The XRD patterns of the CdO nanostructure showed diffraction peaks absorbed at 2θ values (Fig.…”

Diamond as Advanced Material for Scanning Probe Microscopy Tips

“…For example, the detection of dopamine and ascorbic acid at a glassy carbon electrode is impeded, as the formal oxidation potentials (i.e., 755 mV for dopamine and 766 mV for ascorbic acid) lead to overlapping signals. Dopamine detection was shown using differential pulse voltammetry at BDD electrodes in the presence of high concentrations of ascorbic acid revealing individual peaks for both molecules 99. The detection of dopamine at relevant concentrations could also be demonstrated during BDD‐AFM‐SECM experiments, as shown in Figure 6D for bulk calibration experiments, which may in future enable localized measurements of dopamine while imaging live cells.…”

Diamond Ultramicro- and Nano-electrode Arrays

“…We have shown that dopamine can be sensitively and selectively detected on diamond ultramicroelectrode arrays in the presence of ascorbic acid [55]. Compared with the results shown on other diamond electrode (including macro-sized electrodes [60][61][62][63][64][65], microelectrode array [66], ultramicroelectrode arrays [47,48], and diamond nanograss [67]), on diamond ultramicroelectrode arrays we achieved the lowest detection limit (1.0 nM) for dopamine detection, which is 50-100 times lower than that reported [55]. Diamond ultramicroelectrode arrays are thus promising for the detection of low concentrated dopamine (0.01-1 µM) in biological samples individually or in the presence of other similar compounds such as ascorbic acid.…”