Differential pulse voltammetric studies of ethidium bromide binding to DNA

Minasyan, Seyran H.; Tavadyan, L. A.; Antonyan, Ara P.; Davtyan, Hayk G.; Parsadanyan, Marine A.; Vardevanyan, P. O.

doi:10.1016/j.bioelechem.2005.03.006

Cited by 34 publications

(25 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It was shown earlier that EtBr apart from intercalation binds to DNA by semi-intercalation mode as well (group of EtBr molecule aromatic rings is inserted into plane between neighboring bases in one of DNA chains, moreover, semi-intercalation into two opposite chains is simultaneously possible). As it was shown, 21,24 the values of K corresponding to intercalation and semi-intercalation binding modes di®er from each other by almost 10 times. Such a result is also obtained via SWV method which is in good correspondence to analogous values presented in some works.…”

Section: Discussionmentioning

confidence: 85%

“…By the virtue of DPV data, the quantitative analysis was carried out: values of the binding parameters K and n were determined for two modes of EtBr binding to DNA. 24 In this work, an analogous study of interaction of two various ligands À À À EtBr and H33258 with DNA by SWV method was carried out at 2, 20 and 150 mM ionic strengths of the solution taking into consideration that this method is much more sensitive compared to DPV and allows to carry out experiments at lower concentrations of reagents (10 À5 À 10 À6 M). SW voltammograms of H33258 (A) and EtBr (B) in the absence and presence of DNA at 2 mM ionic strength of solution are presented in Fig.…”

Section: Resultsmentioning

confidence: 99%

“…These data are in correspondence to the results obtained by DPV method. 24 From the titration data values of r/C f and r were calculated. Using Eqs.…”

Section: Resultsmentioning

confidence: 99%

“…Titrations of solutions of ligands by DNA solution as well as all procedures of preparation of electrodes were carried out according to the method described by Minasyan et al 24 Electrochemical measurements of DNA complexes with both ligands have been carried out 10 times independently. Each time new samples were prepared, i.e., the same sample was not measured more than one time.…”

Section: Methodsmentioning

confidence: 99%

“…18,19 The voltammetry methods of investigations of DNA binding to various molecules can provide a useful complement to spectroscopic methods, e.g., for nonabsorbing species, and yield in information about the mechanism of binding. [21][22][23][24] Among voltammetry methods, square wave voltammetry (SWV) is one of the most sensitive ones, which permits carrying out measurements at low concentrations of reagents and more precisely¯nding out various types of interactions of ligands with DNA. The aim of the given work is to study the interaction of EtBr and H33258 with DNA by SWV method in NaCl water solution at di®erent ionic strengths, to reveal the binding modes of ligands with DNA, to determine values of thermodynamic parameters K and n for di®erent modes of the binding, to¯nd out the e®ect of solution ionic strength on the binding peculiarities of studying ligands to DNA.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Exploring the Interaction of Ethidium Bromide and HOECHST 33258 with DNA by Means of Electrochemical Approach

Tavadyan

Minasyan

Kocharyan

et al. 2017

Biophys. Rev. Lett.

View full text Add to dashboard Cite

The binding of both ethidium bromide (EtBr) and Hoechst 33258 (H33258) to calf thymus DNA di®ering by the mechanism at di®erent ionic strengths of NaCl water solutions (2, 20 and 150 mM) has been quantitatively studied by square wave voltammetry (SWV). It was revealed that EtBr binds to DNA by more than one mode: at the solution ionic strengths 2 and 150 mM, two modes were revealed, at 20 mM À À À three modes. Values of EtBr binding constant (K) to DNA and the number of nucleotides per one binding site (n) for respective binding modes were determined. For H33258 under the same conditions, two binding modes were found and for both these modes values of K and n were determined. Dependence of the binding types and parameters on the solution ionic strength by SWV method was revealed, which di®ers by the binding type of EtBr and H33258. The obtained data are in good accordance with the ones determined by spectroscopic (absorption and°uorescence) and di®erential pulse voltammetry (DPV) methods. The obtained data indicate the sensibility and high precision of SWV method which may be applied to studies on interaction of di®erent ligands with DNA, which reveal some peculiarities of their binding to DNA that are hidden and are not found by other methods.

show abstract

Section: Discussionmentioning

confidence: 85%

Section: Resultsmentioning

confidence: 99%

“…These data are in correspondence to the results obtained by DPV method. 24 From the titration data values of r/C f and r were calculated. Using Eqs.…”

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Exploring the Interaction of Ethidium Bromide and HOECHST 33258 with DNA by Means of Electrochemical Approach

Tavadyan

Minasyan

Kocharyan

et al. 2017

Biophys. Rev. Lett.

View full text Add to dashboard Cite

show abstract

Photoinduced Intercalation and Coordination of a Dirhodium Complex to DNA: Dual DNA Binding

et al. 2014

View full text Add to dashboard Cite

Two new complexes, cis-H,H-[Rh2 (OCCH3 NH)2 (LL)(CH3 CN)2 ](2+) , where LL=bpy (2, bpy=2,2'-bipyridine) and dppz (3, dppz=dipyrido[3,2-a:2',3'-c]phenazine), were prepared from the reaction of cis-H,H-[Rh2 (OCCH3 NH)2 (CH3 CN)6 ](2+) (1) with the corresponding bidentate ligand. The bpy and dppz ligands chelate to the same rhodium atom and are positioned trans to the amidato N atoms, as determined by the single crystal X-ray structure of 2. Irradiation of 2 and 3 with visible light in water results in the exchange of one CH3 CNeq ligand for an H2 O molecule with quantum yields, Φ400 , of 0.040 and 0.044, respectively (λirr =400 nm). The identities of the photoproducts of 2 and 3 were determined to be cis-H,H-[Rh2 (OCCH3 NH)2 (L)(H2 O)(CH3 CN)](2+) , where L is bpy (4) and dppz (5), respectively. Mobility shift assays show that 4 crosslinks double-stranded DNA, and ESI-MS experiments indicate that both 4 and 5 form covalent adducts with single-stranded DNA. In addition, relative viscosity and 2D NMR experiments show that the dppz ligand of 5 also intercalates into DNA upon irradiation, making 3 a dual-binding agent that both intercalates and covalently binds to DNA upon the absorption of visible light.

show abstract

Square‐Wave Voltammetry as a Tool for Investigation of Doxorubicin Interactions with DNA Isolated from Neuroblastoma Cells

et al. 2009

View full text Add to dashboard Cite

We investigated ethidium bromide intercalation into DNA molecule as a model system to test square-wave voltammetry (SWV) as a suitable method for this purpose We found that 0.13 mg EtBr intercalates into 1 mg dsDNA in average. Further, SWV was utilized for investigation of doxorubicin-DNA interactions. Intercalated doxorubicin reduced observed dsDNA cytosine and adenine (CA) signal, but also provided new signal called DOXO at À 0.35 V. This phenomenon was observed at both single and double stranded DNA standards. We also employed adsorptive transfer stripping technique coupled with SWV for study of doxorubicin-DNA interactions. Doxorubicin intercalation into dsDNA molecule adsorbed onto working electrode was fast, because we observed considerable changes in CA and DOXO signals after 360 s. Finally, we detected doxorubicin-DNA adducts formed in doxorubicin treated neuroblastoma cells.

show abstract

Differential pulse voltammetric studies of ethidium bromide binding to DNA

Cited by 34 publications

References 36 publications

Exploring the Interaction of Ethidium Bromide and HOECHST 33258 with DNA by Means of Electrochemical Approach

Exploring the Interaction of Ethidium Bromide and HOECHST 33258 with DNA by Means of Electrochemical Approach

Photoinduced Intercalation and Coordination of a Dirhodium Complex to DNA: Dual DNA Binding

Square‐Wave Voltammetry as a Tool for Investigation of Doxorubicin Interactions with DNA Isolated from Neuroblastoma Cells

Contact Info

Product

Resources

About