Differential Recognition of
            <i>Vibrio parahaemolyticus</i>
            OmpU by Toll-Like Receptors in Monocytes and Macrophages for the Induction of Proinflammatory Responses

Gulati, Aakanksha; Kumar, Rajesh; Mukhopadhaya, Arunika

doi:10.1128/iai.00809-18

Cited by 17 publications

(7 citation statements)

References 38 publications

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“…To explore the effects of different doses of 11S and SB supplementation on the disease resistance of the hybrid grouper, we conducted a one-week challenge test experiment after the end of the 8-week breeding experiment. As a gram-negative bacterium, Vibrio parahaemolyticus has a wide distribution, mainly living in seawater, fish, shrimp, shells, and crustaceans ( 24 ). It usually causes gastrointestinal infections and extra-intestinal infections in aquatic animals.…”

Section: Discussionmentioning

confidence: 99%

MHC II-PI3K/Akt/mTOR Signaling Pathway Regulates Intestinal Immune Response Induced by Soy Glycinin in Hybrid Grouper: Protective Effects of Sodium Butyrate

et al. 2021

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Soy glycinin (11S) is involved in immune regulation. As an additive, sodium butyrate (SB) can relieve inflammation caused by 11S. To further delve into the mechanisms. A diet containing 50% fishmeal was the control group (FM group), and the experimental groups consisted of the FM group baseline plus 2% glycinin (GL group), 8% glycinin (GH group), and 8% glycinin + 0.13% sodium butyrate (GH-SB group). The specific growth ratio (SGR), feed utilization, and density of distal intestinal (DI) type II mucous cells were increased in the GL group. In the serum, IFN-γ was significantly upregulated in the GL group, and IgG and IL-1β were upregulated in the GH group. IgG, IL-1β, and TNF-α in the GH-SB group were significantly downregulated compared to those in the GH group. The mRNA levels of mTOR C1, mTOR C2, and Deptor were upregulated in the GL, GH, and GH-SB groups in the DI compared with those in the FM group, while the mRNA levels of mTOR C1 and Deptor in the GH group were higher than those in the GL and GH-SB groups. 4E-BP1, RICTOR, PRR5, MHC II, and CD4 were upregulated in the GH group. TSC1, mLST8, and NFY mRNA levels in the GL and GH-SB groups were upregulated compared with those in the FM and GH groups. Western blotting showed P-PI3KSer294/T-PI3K, P-AktSer473/T-Akt, and P-mTORSer2448/T-mTOR were upregulated in the GH group. Collectively, our results demonstrate that low-dose 11S could improve serum immune by secreting IFN-γ. The overexpression of IgG and IL-1β is the reason that high-dose 11S reduces serum immune function, and supplementing SB can suppress this overexpression. Low-dose 11S can block the relationship between PI3K and mTOR C2. It can also inhibit the expression of 4E-BP1 through mTOR C1. High-dose 11S upregulates 4E-BP2 through mTOR C1, aggravating intestinal inflammation. SB could relieve inflammation by blocking PI3K/mTOR C2 and inhibiting 4E-BP2. Generally speaking, the hybrid grouper obtained different serum and DI immune responses under different doses of 11S, and these responses were ultimately manifested in growth performance. SB can effectively enhance serum immunity and relieve intestinal inflammation caused by high dose 11S.

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Section: Discussionmentioning

confidence: 99%

MHC II-PI3K/Akt/mTOR Signaling Pathway Regulates Intestinal Immune Response Induced by Soy Glycinin in Hybrid Grouper: Protective Effects of Sodium Butyrate

et al. 2021

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“…The OmpU, one of the major outer membrane porins of V . parahaemolyticus , is recognized by the Toll-like receptor 1/2 (TLR-1/2) heterodimer in THP-1 monocytes [ 112 ]. So, a docking study was also performed to analyze the affinity between the designed construct and human TLR-1/2 heterodimer.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, it was done to explore the binding affinity of promiscuous epitopes with different HLA alleles including HLA-DRB1 � 03:01 (1A6A), (HLA-DRB5 � 01:01 (1H15), HLA-DRB3 � 01:01 (2Q6W), HLA-DRB1 � 04:01 (2SEB), HLA-DRB1 � 01:01 (2FSE), and HLA-DRB3 � 02:02 (3C5J). The OmpU, one of the major outer membrane porins of V. parahaemolyticus, is recognized by the Toll-like receptor 1/2 (TLR-1/2) heterodimer in THP-1 monocytes [112]. So, a docking study was also performed to analyze the affinity between the designed construct and human TLR-1/ 2 heterodimer.…”

Section: Plos Onementioning

confidence: 99%

Comprehensive genome based analysis of Vibrio parahaemolyticus for identifying novel drug and vaccine molecules: Subtractive proteomics and vaccinomics approach

et al. 2020

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Multidrug-resistant Vibrio parahaemolyticus has become a significant public health concern. The development of effective drugs and vaccines against Vibrio parahaemolyticus is the current research priority. Thus, we aimed to find out effective drug and vaccine targets using a comprehensive genome-based analysis. A total of 4822 proteins were screened from V. parahaemolyticus proteome. Among 16 novel cytoplasmic proteins, 'VIBPA Type II secretion system protein L' and 'VIBPA Putative fimbrial protein Z' were subjected to molecular docking with 350 human metabolites, which revealed that Eliglustat, Simvastatin and Hydroxocobalamin were the top drug molecules considering free binding energy. On the contrary, 'Sensor histidine protein kinase UhpB' and 'Flagellar hook-associated protein of 25 novel membrane proteins were subjected to T-cell and B-cell epitope prediction, antigenicity testing, transmembrane topology screening, allergenicity and toxicity assessment, population coverage analysis and molecular docking analysis to generate the most immunogenic epitopes. Three subunit vaccines were constructed by the combination of highly antigenic epitopes along with suitable adjuvant, PADRE sequence and linkers. The designed vaccine constructs (V1, V2, V3) were analyzed by their physiochemical properties and molecular docking with MHC molecules-results suggested that the V1 is superior. Besides, the binding affinity of human TLR-1/2 heterodimer and construct V1 could be biologically significant in the development of the vaccine repertoire. The vaccine-receptor complex exhibited deformability at a minimum level that also strengthened our prediction. The optimized codons of the designed construct was cloned into pET28a(+) vector of E. coli strain K12. However, the predicted drug molecules and vaccine constructs could be further studied using model animals to combat V. parahaemolyticus associated infections.

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“…Patients, with diarrhoea caused by V. parahaemolyticus, incited an acute inflammation, including nitric oxide (NO) production, high level of tumour necrosis factor alpha (TNF-α), an increase of macrophages, and neutrophils (Qadri et al, 2003). In addition, the outer membrane protein (OmpU) of V. parahaemolyticus (VpOmpU) could activate macrophages and monocytes to induce the production of pro-inflammatory cytokines, including interleukin-6 (IL-6), TNF-α in human monocytes, and IL-6, TNF-α, NO production in murine macrophages (Gulati et al, 2019). Therefore, it is necessary to antagonize the inflammatory cytokine response to protect the body from pathogen infection.…”

mentioning

confidence: 99%

Chloroquine potentially modulated innate immune response to Vibrio parahaemolyticus in RAW 264.7 macrophages

Qiao

Lin

et al. 2021

Food and Agricultural Immunology

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Vibrio parahaemolyticus, as a vibriosis, causes huge losses to the aquaculture industry, food poisoning in humans and activates macrophages to induce pro-inflammatory cytokines. Chloroquine (CQ), as an anti-inflammatory property, this study aimed to investigate the effect of CQ on inflammatory response to V. parahaemolyticus in RAW 264.7 macrophages. The result showed that V. parahaemolyticus induced the production of T-cell co-stimulatory molecules (CD80, CD86, CD40 and MHC II), and increased the production and mRNA expression of proinflammatory, anti-inflammatory cytokines and type I interferons (IFNs). Meanwhile, the stimulated macrophages elevated cellular stress-released reactive oxygen species and nitric oxide synthase to trigger an antimicrobial response, and effectively reduced the accumulation of acetylated low-density lipoprotein and neutral lipids. Finally, we found that chloroquine (CQ) was involved in inhibiting inflammatory cytokine expression in response to V. parahaemolyticus. These results suggested that CQ potentially attenuated the inflammatory cytokine expression in Gramnegative bacterium-activated macrophages.

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Differential Recognition of Vibrio parahaemolyticus OmpU by Toll-Like Receptors in Monocytes and Macrophages for the Induction of Proinflammatory Responses

Cited by 17 publications

References 38 publications

MHC II-PI3K/Akt/mTOR Signaling Pathway Regulates Intestinal Immune Response Induced by Soy Glycinin in Hybrid Grouper: Protective Effects of Sodium Butyrate

MHC II-PI3K/Akt/mTOR Signaling Pathway Regulates Intestinal Immune Response Induced by Soy Glycinin in Hybrid Grouper: Protective Effects of Sodium Butyrate

Comprehensive genome based analysis of Vibrio parahaemolyticus for identifying novel drug and vaccine molecules: Subtractive proteomics and vaccinomics approach

Chloroquine potentially modulated innate immune response to Vibrio parahaemolyticus in RAW 264.7 macrophages

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