Differential regulation of cytokine and chemokine production in lipopolysaccharide-induced tolerance and priming

Peck, Octavia M; Williams, David L.; Breuel, Kevin F.; Kalbfleisch, John H.; Tempel, G; Teti, Giuseppe; Cook, James A.

doi:10.1016/j.cyto.2004.02.011

Cited by 24 publications

(12 citation statements)

References 45 publications

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“…ET was defined as a reduction of TNF-α by equal to or greater than 75% compared to non-tolerized cells. In previous studies, TNF-α has been used as a phenotypic marker of ET and its production was decreased by 98-100% in most studies (Allen et al 1996;Peck et al 2004). TNF-α production peaks at 90-120 minutes after LPS exposure and was measured at that time in referenced studies (Allen et al 1996;Peck et al 2004).…”

Section: Chapter 4 Discussionmentioning

confidence: 99%

“…hours (Peck et al 2004). In tolerized cells, production of TNF-α was decreased to less than 1% of non-tolerized cells (Peck et al 2004).…”

Section: In Vitro Etmentioning

confidence: 99%

“…IL-6 production was increased in tolerized cells compared to non-tolerized cells. However, no change was observed in IL-1β and IL-8 production (Peck et al 2004). In another study, human dendritic cells were pretreated with 1 ng/ml LPS (Salmonella typhimurium) for 24 hours, and then stimulated with 1 µg/ml of LPS (Karp et al 1998).…”

Section: In Vitro Etmentioning

confidence: 99%

“…In tolerized cells, production of TNF-α was decreased to less than 1% of non-tolerized cells (Peck et al 2004). IL-6 production was increased in tolerized cells compared to non-tolerized cells.…”

Section: In Vitro Etmentioning

confidence: 99%

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Induction and characterization of endotoxin tolerance in equine peripheral blood mononuclear cells in vitro

Frellstedt

McKenzie

Barrett

et al. 2012

Veterinary Immunology and Immunopathology

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Endotoxemia is responsible for severe illness in horses. Individuals can become unresponsive to the endotoxin molecule after an initial exposure; this phenomenon has been called developing a state of 'endotoxin tolerance' (ET). ET has been induced in horses in vivo; however, cytokine expression associated with ET has not been investigated. The purpose of this study was to develop and validate a method for inducing ET in equine peripheral blood mononuclear cells (PBMCs) in vitro, and to describe the cytokine profile which is associated with the ET.Blood was collected from 6 healthy horses and PBMCs were isolated. ET was induced by culturing cells with three concentrations of endotoxin given to induce ET, and evaluated after a second dose of endotoxin given to challenge the cells. The relative mRNA expression of IL-10 and IL-12 was measured by use of quantitative PCR.ET was induced in all cells (n=6) exposed to the 2-step endotoxin challenge. In PBMCs treated with 1.0 ng/ml of endotoxin followed by challenge with 10 ng/ml of endotoxin, the relative mRNA expression of IL-10 in tolerized cells was not different from positive control cells. In contrast, the relative mRNA expression of IL-12 in tolerized cells was decreased by 15-fold after the second endotoxin challenge compared with positive control cells.This experiment demonstrated a reliable method for the ex vivo induction of ET in equine PBMCs. A marked suppression of IL-12 production is associated with ET. The production of IL-10 was not altered in ET in our model.

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Section: Chapter 4 Discussionmentioning

confidence: 99%

“…hours (Peck et al 2004). In tolerized cells, production of TNF-α was decreased to less than 1% of non-tolerized cells (Peck et al 2004).…”

Section: In Vitro Etmentioning

confidence: 99%

Section: In Vitro Etmentioning

confidence: 99%

Section: In Vitro Etmentioning

confidence: 99%

See 2 more Smart Citations

Induction and characterization of endotoxin tolerance in equine peripheral blood mononuclear cells in vitro

Frellstedt

McKenzie

Barrett

et al. 2012

Veterinary Immunology and Immunopathology

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show abstract

“…In addition to their direct effector functions, including phagocytosis of microbes and release of antimicrobial compounds, phagocytes can release a variety of pro-inflammatory mediators that control the acute phase response and that affect multiple events leading to activation of the cellular and humoral defenses [3,4]. Amongst these mediators are a number of proinflammatory cytokines, such as tumor necrosis factor a (TNF-a), interleukin-1b (IL-1b), IL-6 and the chemokine IL-8 [5]. IL-8, or CXCL8, belongs to the CXC family of chemokines, so called because of the arrangement of the first two cysteine residues that have an amino acid residue (X) between them [6].…”

Section: Introductionmentioning

confidence: 99%

The biological effects of rainbow trout (Oncorhynchus mykiss) recombinant interleukin-8

Harun

Zou

Zhang

et al. 2008

Developmental & Comparative Immunology

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Chemiluminescence of non‐differentiated THP‐1 promonocytes: developing an assay for screening anti‐inflammatory milk proteins and peptides

et al. 2011

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Human leukemic THP-1 promonocytes are widely used as a model for peripheral blood monocytes. However, superoxide production during respiratory burst (RB) of non-differentiated THP-1 (nd-THP-1) cells is very low. Here we present a rapid and low-cost method for measuring the chemiluminescence (CL) of opsonized zymosan (OZ) induced RB which allows detection of Escherichia coli lipopolysaccharide (LPS) induced priming of nd-THP-1 cells on the basis of CL reaction kinetics. Maximum CL intensity obtained was 2.20 ± 0.25 and 1.30 ± 0.11 relative light units, while CL peak time was achieved at 18.1 ± 2.6 and 28.7 ± 1.3 min in primed and non-primed cells, respectively. The priming of nd-THP-1 cells with LPS evoked typical TNF-α and IL-6 production. We tested the effects of bovine lactoferrin and protein fractions from Lactobacillus helveticus BGRA43 fermented milk for potential anti-inflammatory effects on LPS primed nd-THP-1 cells. Four fractions were found to inhibit the OZ-induced CL in a dose-dependent manner (IC(50) 3-30 µg/mL), while lactoferrin inhibited CL to a lesser extent (IC(50) 270 µg/mL). These results suggest that measuring CL response of nd-THP-1 cells can serve as a method for screening anti-inflammatory compounds which could be used in reducing the risk of phagocyte-mediated inflammatory diseases.

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Differential regulation of cytokine and chemokine production in lipopolysaccharide-induced tolerance and priming

Cited by 24 publications

References 45 publications

Induction and characterization of endotoxin tolerance in equine peripheral blood mononuclear cells in vitro

Induction and characterization of endotoxin tolerance in equine peripheral blood mononuclear cells in vitro

The biological effects of rainbow trout (Oncorhynchus mykiss) recombinant interleukin-8

Chemiluminescence of non‐differentiated THP‐1 promonocytes: developing an assay for screening anti‐inflammatory milk proteins and peptides

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