Differential Regulation of the Production of Reactive Oxygen Species in Th1 Cytokine–Treated Thyroid Cells

Colin, Idesbald; Poncin, Sylvie; Lévêque, Philippe; Gallez, Bernard; Gérard, A

doi:10.1089/thy.2013.0142

Cited by 18 publications

(12 citation statements)

References 67 publications

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“…The levels of IL1β in group II were significantly decreased (Ме 3.90 pg/mL; range: 2.35–42.24 pg/mL) in comparison with those observed in group I (Me 15.42 pg/mL; range: 5.80–38.20 pg/mL; p < 0.01). This finding is consistent with recent research data on the immune regulatory properties of IL2 aimed at the differentiation of regulatory T cells [16, 17]. The balance of IFNγ/IL10 in animal blood serum increased to 1.9 and 1.2 in the groups with hypothyroidism and thyrotoxicosis, respectively.…”

Section: Resultssupporting

confidence: 93%

Roles of Thyroid Hormones, Mast Cells, and Inflammatory Mediators in the Initiation and Progression of Autoimmune Thyroid Diseases

Здор¹,

Гельцер²,

Елисейкина³

et al. 2020

Int Arch Allergy Immunol

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Background: Interrelation between thyrocytes and immunocytes has been established. However, the roles of mast cells and thyroid hormones in the triggering mechanism of thyroid autoimmune processes have been insufficiently investigated. This study evaluated the role of thyroid hormones and mast cells in the mechanisms of losing tolerance to thyroid autoantigens. Materials and Methods: Two groups of patients were studied: patients with Graves' disease and patients with nodular euthyroid goiter. Wistar rats with induced exogenous hypothyroidism, thyrotoxicosis, and thyrotoxicosis in parallel with administration of interleukin-2 were used. The levels of hormones, autoantibodies, and cytokines in the serum and thyroid tissue were analyzed through the enzyme-linked immunosorbent assay. Morphological verification was performed through the immune-his

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Section: Resultssupporting

confidence: 93%

Roles of Thyroid Hormones, Mast Cells, and Inflammatory Mediators in the Initiation and Progression of Autoimmune Thyroid Diseases

Здор¹,

Гельцер²,

Елисейкина³

et al. 2020

Int Arch Allergy Immunol

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“…Our study shows that NOX4 was increased in human thyroid cells treated with Th1 cytokines, as well as in HT thyroid samples, while NOX2 expression was not modified. This is in contrast to the results reported by Colin et al (2014) [10] and this could be explained by the fact that we used primary cultures of human thyroid cells rather than PCCL3 cell lines. Colin et al (2014) [10] analyzed the NOX2 protein level by immunohistochemistry on two HT patients without making the distinction between the different types of follicles.…”

Section: Discussioncontrasting

confidence: 82%

“…Moreover, Werion et al (2016) highlighted that a decrease in PPARγ, a well-known regulator of Cav-1 and also of the antioxidant catalase, is a key event in HT pathogenesis [9]. Colin et al (2014) proposed that ROS production induced by Th1 cytokines in thyroid cell lines results from NOX2 upregulation and, thus, superoxide overproduction [10]. They did not observe any modification of NOX4 expression in the PCCL3 cell lines and in the thyroid samples of two HT patients.…”

Section: Introductionmentioning

confidence: 98%

Oxidative Stress-Induced Sirtuin1 Downregulation Correlates to HIF-1α, GLUT-1, and VEGF-A Upregulation in Th1 Autoimmune Hashimoto’s Thyroiditis

Hepp

Wérion

Greef

et al. 2021

IJMS

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In Hashimoto’s thyroiditis (HT), oxidative stress (OS) is driven by Th1 cytokines’ response interfering with the normal function of thyrocytes. OS results from an imbalance between an excessive production of reactive oxygen species (ROS) and a lowering of antioxidant production. Moreover, OS has been shown to inhibit Sirtuin 1 (SIRT1), which is able to prevent hypoxia-inducible factor (HIF)-1α stabilization. The aims of this study were to determine the involvement of NADPH-oxidases (NOX), SIRT1, and HIF-1α in HT pathophysiology as well as the status of antioxidant proteins such as peroxiredoxin 1 (PRDX1), catalase, and superoxide dismutase 1 (SOD1). The protein expressions of NOX2, NOX4, antioxidant enzymes, SIRT1, and HIF-1α, as well as glucose transporter-1 (GLUT-1) and vascular endothelial growth factor A (VEGF-A), were analyzed by Western blot in primary cultures of human thyrocytes that were or were not incubated with Th1 cytokines. The same proteins were also analyzed by immunohistochemistry in thyroid samples from control and HT patients. In human thyrocytes incubated with Th1 cytokines, NOX4 expression was increased whereas antioxidants, such as PRDX1, catalase, and SOD1, were reduced. Th1 cytokines also induced a significant decrease of SIRT1 protein expression associated with an upregulation of HIF-1α, GLUT-1, and VEGF-A proteins. With the exception of PRDX1 and SOD1, similar results were obtained in HT thyroids. OS due to an increase of ROS produced by NOX4 and a loss of antioxidant defenses (PRDX1, catalase, SOD1) correlates to a reduction of SIRT1 and an upregulation of HIF 1α, GLUT-1, and VEGF-A. Our study placed SIRT1 as a key regulator of OS and we, therefore, believe it could be considered as a potential therapeutic target in HT.

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“…First, inflammation directly increases the level of hydrogen peroxide in thyroid epithelial cells, and second, it activates NOX enzyme in T and B lymphocytes, which increases ROS production (22,23). In fact, the Th1 cytokines (predominant in HT) are known to decrease DUOX and thus H 2 O 2 production but to increase NOX2 (without affecting NOX4) (24). Another reason for the determination of a low TAS level in the hypothyroidism group compared to other groups may be a decreased thyroid hormone level, because thyroid hormones are known to affect synthesis and bioactivity of antioxidant enzymes (25,26).…”

Section: European Journal Of Endocrinologymentioning

confidence: 99%

The relationship between oxidative stress and autoimmunity in Hashimoto's thyroiditis

Ateş

Yılmaz

Altay

et al. 2015

European Journal of Endocrinology

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Objective: We have aimed to study the relation between Hashimoto's thyroiditis (HT) and thyroid autoantibodies and oxidative stress parameters in euthyroid, subclinical and overt hypothyroid stages. Design and methods: A total of 124 patients were included in the study; 93 of whom were newly diagnosed with HT (31 patients in each of the euthyroid, subclinical hypothyroid and overt hypothyroid subgroups), aged over 18 and had not received any prior treatment and 31 of whom were healthy volunteers. Results: Total oxidant status (TOS) and oxidative stress index (OSI) levels were higher, and total antioxidant status (TAS) and total thiol and arylesterase levels were lower in the overt hypothyroid group compared to other groups. TOS and OSI levels increased, and TAS levels decreased significantly in each phase from euthyroid, subclinical hypothyroid, to overt hypothyroid subgroups among HT patients. There was a negative correlation between TAS, log (paraoxonase1) and paraoxonase1/HDL and anti-thyroid peroxidase and a negative correlation between anti-thyroglobulin and total thiol. It was also determined that overt hypothroidism was an individual predictor that effects all of the oxidative stress parameters, but not total thiol, levels. Conclusion: Our results suggest that oxidative stress increases continuously during the development of subclinical hypothyroidism and overt hypothyroidism in patients with HT. To determine whether this is a cause or result, randomized, controlled trials that study the effect of antioxidant treatment on the development of overt hypothyroidism and its consequences, e.g., increase in total cholesterol levels, may be performed in euthyroid and/or subclinical hypothyroid patients with HT.

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Differential Regulation of the Production of Reactive Oxygen Species in Th1 Cytokine–Treated Thyroid Cells

Cited by 18 publications

References 67 publications

Roles of Thyroid Hormones, Mast Cells, and Inflammatory Mediators in the Initiation and Progression of Autoimmune Thyroid Diseases

Roles of Thyroid Hormones, Mast Cells, and Inflammatory Mediators in the Initiation and Progression of Autoimmune Thyroid Diseases

Oxidative Stress-Induced Sirtuin1 Downregulation Correlates to HIF-1α, GLUT-1, and VEGF-A Upregulation in Th1 Autoimmune Hashimoto’s Thyroiditis

The relationship between oxidative stress and autoimmunity in Hashimoto's thyroiditis

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