2019
DOI: 10.1242/jcs.228486
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Differential requirements for the EF-hand domains of human centrin 2 in primary ciliogenesis and nucleotide excision repair

Abstract: Centrin 2 is a small conserved calcium-binding protein that localizes to the centriolar distal lumen in human cells. It is required for efficient primary ciliogenesis and nucleotide excision repair (NER). Centrin 2 forms part of the xeroderma pigmentosum group C protein complex. To explore how centrin 2 contributes to these distinct processes, we mutated the four calcium-binding EF-hand domains of human centrin 2. Centrin 2 in which all four EF-hands had been mutated to ablate calcium binding (4DA mutant) was … Show more

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Cited by 15 publications
(8 citation statements)
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“…However, our as-yet-unsuccessful attempts to directly detect CN localization at centrosomes (data not shown) suggest that CN-centrosome interactions are limited, transient, and/or temporally regulated. Investigations of Ca 2+ signaling at centrosomes, which contain multiple Ca 2+ -and/or calmodulin-binding proteins (Galletta et al, 2014;Khouj et al, 2019;Spektor et al, 2007;Tsang et al, 2006), and cilia, which constitute a distinct Ca 2+ compartment (Delling et al, 2013(Delling et al, , 2016, are ongoing. Our findings point to CN as a likely downstream effector of Ca 2+ signals at these organelles.…”
Section: Discussionmentioning
confidence: 99%
“…However, our as-yet-unsuccessful attempts to directly detect CN localization at centrosomes (data not shown) suggest that CN-centrosome interactions are limited, transient, and/or temporally regulated. Investigations of Ca 2+ signaling at centrosomes, which contain multiple Ca 2+ -and/or calmodulin-binding proteins (Galletta et al, 2014;Khouj et al, 2019;Spektor et al, 2007;Tsang et al, 2006), and cilia, which constitute a distinct Ca 2+ compartment (Delling et al, 2013(Delling et al, , 2016, are ongoing. Our findings point to CN as a likely downstream effector of Ca 2+ signals at these organelles.…”
Section: Discussionmentioning
confidence: 99%
“…As shown in Fig. S1, Centrin-2 and POC5 proximity interactomes were enriched for GO categories that are relevant for their published functions during centrosomal and/or nuclear biological processes and related cellular compartments (Azimzadeh et al, 2009; Dantas et al, 2013; Heydeck et al, 2020; Khouj et al, 2019; Resendes et al, 2008; Salisbury et al, 2002; Steib et al, 2020; Yang et al, 2010; Ying et al, 2019). For example, the highly enriched GO categories for biological processes include cell division, protein folding, mRNA splicing, and chromatin remodeling for Centrin-2 and centriole replication, protein localization to centrosome and cilium assembly for POC5 (Fig.…”
Section: Resultsmentioning
confidence: 92%
“…Ca 2+ signals have been observed at centrosomes (Helassa et al, 2019), where they activate downstream effectors like CaM (Plotnikova et al, 2012) and centrin. In fact, centrin requires Ca 2+ to localize to centrosomes and to interact with the structural protein POC5 (Khouj et al, 2019). CN binds directly to POC5 through a PxIxIT motif and alters POC5 distribution within the centriole, suggesting that it may directly regulate this protein despite our inability to detect CN-dependent changes in POC5 phosphorylation in vivo .…”
Section: Resultsmentioning
confidence: 99%