Differential resistance of oilseed rape cultivars (Brassica napus ssp. oleifera) to Verticillium longisporum infection is affected by rhizosphere colonisation with antagonistic bacteria, Serratia plymuthica and Pseudomonas chlororaphis

Abuamsha, Ruba; Salman, Mazen; Ehlers, Ralf‐Udo

doi:10.1007/s10526-010-9308-8

Cited by 17 publications

(13 citation statements)

References 35 publications

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“…Besides its biocontrol effect on V. dahliae in eggplant and potato, the bacterial BCA P. alvei K-165 reduced root discoloration and hypocotyl lesions caused by the black root rot fungus Thielaviopsis basicola on cotton seedlings (Tjamos et al, 2004; Schoina et al, 2011). Pseudomonas chlororaphis MA 342, which suppressed V. longisporum in oilseed rape, furthermore controls a wide range of cereal seed-borne diseases and is the active organism in the registered products Cedomon® and Cerall® (Johnsson et al, 1998; Abuamsha et al, 2011). …”

Section: What Are the Key Factors In The Process From Selection Of Thmentioning

confidence: 99%

Desirable Traits of a Good Biocontrol Agent against Verticillium Wilt

et al. 2017

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The soil-borne fungus Verticillium causes serious vascular disease in a wide variety of annual crops and woody perennials. Verticillium wilt is notoriously difficult to control by conventional methods, so there is great potential for biocontrol to manage this disease. In this study we aimed to review the research about Verticillium biocontrol to get a better understanding of characteristics that are desirable in a biocontrol agent (BCA) against Verticillium wilt. We only considered studies in which the BCAs were tested on plants. Most biocontrol studies were focused on plants of the Solanaceae, Malvaceae, and Brassicaceae and within these families eggplant, cotton, and oilseed rape were the most studied crops. The list of bacterial BCAs with potential against Verticillium was dominated by endophytic Bacillus and Pseudomonas isolates, while non-pathogenic xylem-colonizing Verticillium and Fusarium isolates topped the fungal list. Predominant modes of action involved in biocontrol were inhibition of primary inoculum germination, plant growth promotion, competition and induced resistance. Many BCAs showed in vitro antibiosis and mycoparasitism but these traits were not correlated with activity in vivo and there is no evidence that they play a role in planta. Good BCAs were obtained from soils suppressive to Verticillium wilt, disease suppressive composts, and healthy plants in infested fields. Desirable characteristics in a BCA against Verticillium are the ability to (1) affect the survival or germination of microsclerotia, (2) colonize the xylem and/or cortex and compete with the pathogen for nutrients and/or space, (3) induce resistance responses in the plant and/or (4) promote plant growth. Potential BCAs should be screened in conditions that resemble the field situation to increase the chance of successful use in practice. Furthermore, issues such as large scale production, formulation, preservation conditions, shelf life, and application methods should be considered early in the process of selecting BCAs against Verticillium.

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Section: What Are the Key Factors In The Process From Selection Of Thmentioning

confidence: 99%

Desirable Traits of a Good Biocontrol Agent against Verticillium Wilt

et al. 2017

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“…; Abuamsha et al . ), but only few studies have focused on beneficial fungi (Alstrom ) as potential biological control agents.…”

Section: Introductionmentioning

confidence: 99%

“…One potential strategy is the use of soil beneficial micro-organisms. Several studies have concentrated on beneficial rhizobacteria to control Verticillium wilt (Berg 1996;Gran er et al 2003;Danielsson et al 2007;Debode et al 2007;Abuamsha et al 2011), but only few studies have focused on beneficial fungi (Alstrom 2000) as potential biological control agents.…”

Section: Introductionmentioning

confidence: 99%

The endophyteVerticillium Vt305 protects cauliflower against Verticillium wilt

Tyvaert

França

Debode³

et al. 2014

J Appl Microbiol

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Aims: To investigate the interaction between cauliflower and the isolate Verticillium Vt305, obtained from a field suppressive to Verticillium wilt of cauliflower, and to evaluate the ability of Verticillium Vt305 to control Verticillium wilt of cauliflower caused by V. longisporum. Methods and Results: Single and combined inoculations of Verticillium Vt305 and V. longisporum were performed on cauliflower seedlings. Symptom development was evaluated, and fungal colonization was measured in the roots, hypocotyl and stem with real-time PCR. No symptoms were observed after single inoculation of Verticillium Vt305, although it colonized the plant tissues. Pre-inoculation of Verticillium Vt305 reduced symptom development and colonization of plant tissues by V. longisporum. Conclusions: Verticillium Vt305 is an endophyte on cauliflower plants and showed effective biological control of V. longisporum in controlled conditions. Significance and Impact of the Study: This work can contribute to the development of a sustainable control measure of V. longisporum in Brassicaceae hosts, which is currently not available. Additionally, this study provides evidence for the different roles of Verticillium species present in the agro-ecosystem.

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“…Oilseed rape is a globally important oil crop cultivated for edible oil, biofuel production, and phytoextraction of heavy metals; however, it is susceptible to numerous diseases (28). The rhizospheric environment of this crop has been studied for its potential to harbor biocontrol bacteria (such as Serratia proteamaculans, S. plymuthica, Pseudomonas chlororaphis, P. acidovorans, and P. putida) that can protect the plant against fungal pathogens (29,30). The aims of the present study were to characterize and compare the structure and composition of the root and rhizosphere bacterial and fungal communities of oilseed rape plants and to identify the microbial groups capable of competing for recently fixed carbon and compare these with other less active groups not incorporating a plant assimilate.…”

mentioning

confidence: 99%

Identifying the Active Microbiome Associated with Roots and Rhizosphere Soil of Oilseed Rape

Gkarmiri

Mahmood

Ekblad

et al. 2017

Appl Environ Microbiol

124

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RNA stable isotope probing and high-throughput sequencing were used to characterize the active microbiomes of bacteria and fungi colonizing the roots and rhizosphere soil of oilseed rape to identify taxa assimilating plant-derived carbon following CO labeling. Root- and rhizosphere soil-associated communities of both bacteria and fungi differed from each other, and there were highly significant differences between their DNA- and RNA-based community profiles. ,, ,, ,, and were the most active bacterial phyla in the rhizosphere soil. were more active in roots. The most abundant bacterial genera were well represented in both the C- andC-RNA fractions, while the fungal taxa were more differentiated. ,, and were dominant in roots, whereas and () were dominant in rhizosphere soil. " Nitrososphaera" was enriched in C in rhizosphere soil. and were abundant in theC-RNA fraction of roots; was abundant in both roots and rhizosphere soil and heavilyC enriched. was dominant in rhizosphere soil and less abundant, but wasC enriched in roots. The patterns of colonization and C acquisition revealed in this study assist in identifying microbial taxa that may be superior competitors for plant-derived carbon in the rhizosphere of This microbiome study characterizes the active bacteria and fungi colonizing the roots and rhizosphere soil of using high-throughput sequencing and RNA-stable isotope probing. It identifies taxa assimilating plant-derived carbon following CO labeling and compares these with other less active groups not incorporating a plant assimilate. is an economically and globally important oilseed crop, cultivated for edible oil, biofuel production, and phytoextraction of heavy metals; however, it is susceptible to several diseases. The identification of the fungal and bacterial species successfully competing for plant-derived carbon, enabling them to colonize the roots and rhizosphere soil of this plant, should enable the identification of microorganisms that can be evaluated in more detailed functional studies and ultimately be used to improve plant health and productivity in sustainable agriculture.

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Differential resistance of oilseed rape cultivars (Brassica napus ssp. oleifera) to Verticillium longisporum infection is affected by rhizosphere colonisation with antagonistic bacteria, Serratia plymuthica and Pseudomonas chlororaphis

Cited by 17 publications

References 35 publications

Desirable Traits of a Good Biocontrol Agent against Verticillium Wilt

Desirable Traits of a Good Biocontrol Agent against Verticillium Wilt

The endophyteVerticillium Vt305 protects cauliflower against Verticillium wilt

Identifying the Active Microbiome Associated with Roots and Rhizosphere Soil of Oilseed Rape

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