Differential response of antioxidant systems in leaves and roots of barley subjected to anoxia and post-anoxia

Skutnik, M; Rychter, Anna M.

doi:10.1016/j.jplph.2008.11.010

Cited by 42 publications

(28 citation statements)

References 39 publications

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“…The ratios of GSH/GSSG were found to vary to changes in oxygen concentration in roots (6.1) and leaf cells (14.8) (Skutnik and Rychter 2009). However, such high redox ratios related to GSH were not observed in control samples in the present study.…”

Section: Ros Mediates Beneficial Interactions Between Chloroplasts Ancontrasting

confidence: 75%

Importance of ROS and antioxidant system during the beneficial interactions of mitochondrial metabolism with photosynthetic carbon assimilation

Challabathula

Vishwakarma

Yearla

et al. 2009

Planta

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The present study suggests the importance of reactive oxygen species (ROS) and antioxidant metabolites as biochemical signals during the beneficial interactions of mitochondrial metabolism with photosynthetic carbon assimilation at saturating light and optimal CO2. Changes in steady-state photosynthesis of pea mesophyll protoplasts monitored in the presence of antimycin A [AA, inhibitor of cytochrome oxidase (COX) pathway] and salicylhydroxamic acid [SHAM, inhibitor of alternative oxidase (AOX) pathway] were correlated with total cellular ROS and its scavenging system. Along with superoxide dismutase (SOD) and catalase (CAT), responses of enzymatic components--ascorbate peroxidase (APX), monodehydroascorbate reductase (MDAR), glutathione reductase (GR) and non-enzymatic redox components of ascorbate-glutathione (Asc-GSH) cycle, which play a significant role in scavenging cellular ROS, were examined in the presence of mitochondrial inhibitors. Both AA and SHAM caused marked reduction in photosynthetic carbon assimilation with concomitant rise in total cellular ROS. Restriction of electron transport through COX or AOX pathway had differential effect on ROS generating (SOD), ROS scavenging (CAT and APX) and antioxidant (Asc and GSH) regenerating (MDAR and GR) enzymes. Further, restriction of mitochondrial electron transport decreased redox ratios of both Asc and GSH. However, while decrease in redox ratio of Asc was more prominent in the presence of SHAM in light compared with dark, decrease in redox ratio of GSH was similar in both dark and light. These results suggest that the maintenance of cellular ROS at optimal levels is a prerequisite to sustain high photosynthetic rates which in turn is regulated by respiratory capacities of COX and AOX pathways.

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Section: Ros Mediates Beneficial Interactions Between Chloroplasts Ancontrasting

confidence: 75%

Importance of ROS and antioxidant system during the beneficial interactions of mitochondrial metabolism with photosynthetic carbon assimilation

Challabathula

Vishwakarma

Yearla

et al. 2009

Planta

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“…Moreover, the accumulation of antioxidant enzymes and the synthesis of drought-resistant proteins can increase soluble protein levels in plants [45]. In the present study, the level of soluble proteins first increased and then peaked following treatment with 30% PEG for 12 h, yet decreased following treatment with 40% PEG for 12 h. These discrepant results are similar to those observed by Skutnik and Rychter [46]. The first increase in soluble protein levels in the plants treated with 10%, 20%, and 30% PEG for 12 h indicated that B. humilis maintains a vigorous metabolic level to increase resistance to osmotic stress at the late stage.…”

Section: Discussionsupporting

confidence: 87%

Physiological adaptations to osmotic stress and characterization of a polyethylene glycol-responsive gene in Braya humilis

Wang

Zhao

et al. 2016

Acta Soc Bot Pol

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Braya humilis (Brassicaceae) is a widely distributed plant in arid and semi-arid regions of northern Asia. This plant is well adapted to extremely arid conditions and is a promising candidate species to discover novel drought tolerance strategies. However, not much information about the mechanism(s) mediating drought resistance in this species is currently available. Therefore, the present study aimed to characterize the physiological traits and expression patterns of a polyethylene glycol (PEG)-responsive gene in B. humilis responding to different levels of osmotic stress induced by PEG-6000. Several important physiological parameters were examined, including the levels of relative water content, soluble protein, malondialdehyde, and antioxidant enzyme activity. A tolerance threshold between 20 and 30% PEG-6000 was identified for B. humilis. The water status and oxidative damage below this threshold were maintained at a relatively constant level during the 12 h of treatment. However, once the threshold was exceeded, the water status and oxidative damage were obviously affected after treatment for 4 h. The soluble protein results suggest that B. humilis maintains a vigorous resistance to osmotic stress and that it may play a greater role in osmotic regulation at late stages of stress. Moreover, superoxide dismutase and catalase may be important at preventing oxidative damage in plants at early stages of stress, while peroxidase may be more involved in some biological processes that resist osmotic stress at the late stage, especially in severely damaged plants. Furthermore, a PEG-responsive gene, BhCIPK12, was identified by differential display reverse transcription-polymerase chain reaction (PCR), cloned, and characterized by quantitative real-time PCR. We hypothesized that this gene may play an important role in mediating osmotic stress or drought resistance in plants. Altogether, these results provide valuable insights into the mechanism(s) mediating drought tolerance in B. humilis.

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“…Anoxia caused a significant inhibition of MDHAR, DHAR and GR activities (Biemelt et al 1998) whereas inhibition of GR and APX was observed in corn leaves under prolonged flooding (Yan et al 1996). Recent data have shown that the leaf and root cell ascorbate/glutathione systems react differentially to changes in oxygen concentration (Skutnik and Rychter 2009).…”

Section: Low Oxygen Stressmentioning

confidence: 99%

“…Immediately after the end of an anaerobic exposure, a rapid oxidation of ascorbate and glutathione pool took place in barley leaves, suggesting that these low-mass antioxidants are the "first line" of ROS detoxification under low oxygen stress (Skutnik and Rychter 2009). Conversely, ascorbate and glutathione pools became even more reduced in roots.…”

Section: Low Oxygen Stressmentioning

confidence: 99%

Involvement of AsA/DHA and GSH/GSSG Ratios in Gene and Protein Expression and in the Activation of Defence Mechanisms Under Abiotic Stress Conditions

Fotopoulos

Ziogas

Tanou

et al. 2010

Ascorbate-Glutathione Pathway and Stress Tolerance in Plants

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In a persistently changing environment there are many adverse abiotic stress conditions such as cold, heat, drought, salinity, heavy metal toxicity and oxygen deprivation, which remarkably influence plant growth and crop production. Plant cells produce oxygen radicals and their derivatives, so-called reactive oxygen species (ROS) during various processes associated with abiotic stress. Moreover, the generation of ROS is the main means for higher plants to transmit cellular signalling information concerning the changing environmental conditions. Therefore, plants have evolved inducible redox state-based sensing mechanisms that are activated or amplified in response to adverse environmental conditions. Ascorbate and glutathione, the key cellular redox buffers, are used for both detoxification of ROS and transmission of redox signals. In recent years, it has become clear that abiotic stress conditions induce changes in the reduction/oxidation (redox) state of signalling molecules, which in turn modulate gene and protein expression to increase plant acclimation to abiotic stress. This important redox state-related branch of science has given several clues in understanding the adaptive plant responses to different stressful regimes. In this chapter, an overview of the literature is briefly presented in terms of the main function of ascorbate and glutathione in plant cells. Further more, we describe how important forms of abiotic stress regulate the expression of genes and proteins involved in the ascorbate and glutathione redox sensing system.

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Differential response of antioxidant systems in leaves and roots of barley subjected to anoxia and post-anoxia

Cited by 42 publications

References 39 publications

Importance of ROS and antioxidant system during the beneficial interactions of mitochondrial metabolism with photosynthetic carbon assimilation

Importance of ROS and antioxidant system during the beneficial interactions of mitochondrial metabolism with photosynthetic carbon assimilation

Physiological adaptations to osmotic stress and characterization of a polyethylene glycol-responsive gene in Braya humilis

Involvement of AsA/DHA and GSH/GSSG Ratios in Gene and Protein Expression and in the Activation of Defence Mechanisms Under Abiotic Stress Conditions

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