Differential Response of Liver and Kidney Adenine Nucleotide Translocase and Pyruvate Dehydrogenase Activity to Alloxan Diabetes

Abstract

Adenine nucleotide translocase (EC 3.6.1.3.), pyruvate dehydrogenase (active and total forms, EC 1.2.4.1) and the long chain acyl CoA content were measured in liver and kidney from normal and alloxan-diabetic rats. The long chain acyl CoA content was significantly increased in liver, but not in kidney, in the diabetic group. Adenine nucleotide translocase activity was decreased in liver and raised in the kidney of alloxan-diabetic rats relative to the control group. Pyruvate dehydrogenase (active) was inhibited to a similar degree in both tissues in diabetes. The results are discussed in the light of the possible regulatory role of long chain acyl CoA and the diverse metabolic demands of the two tissues in diabetes.

Effect of streptozotocin‐induced diabetes on oxidative energy metabolism in rat kidney mitochondria. A comparative study of early and late effects

“…It has been reported that the pyruvate dehydrogenase activity in the liver decreases significantly in diabetes [8,13]. Also, treatment with insulin did not reverse the changes in the respiration rates.…”

“…Considerable amount of work has been carried out to evaluate the effect of diabetic state and insulin treatment on oxidative energy metabolism in the liver mitochondria (e.g. Surprisingly, however, hardly any work has been carried out along these lines to examine the energy-linked functions of the kidney mitochondria in diabetic state or following insulin treatment [8,20]. Surprisingly, however, hardly any work has been carried out along these lines to examine the energy-linked functions of the kidney mitochondria in diabetic state or following insulin treatment [8,20].…”

“…Nonenzymatic glycosylation of the basal membrane and accumulation of advanced glycosylated end products in the kidneys is also well recognized [4]. Renal hypertrophy is one of the common and persistent feature of diabetes in humans and in experimental diabetic animal models [5][6][7][8][9][10][11]. The renal enlargement in diabetes is associated with hyper function of the kidneys including increased glomerular filtration rate, Na þ and water resorption and altered Na þ K þ ATPase pump activity Correspondence: Prof Surendra S. Katyare, Department of Biochemistry, Faculty of Science, M. S. University of Baroda, Vadodara, Gujarat 390 002, India.…”

“…for comprehensive review see [18,19]). Surprisingly, however, hardly any work has been carried out along these lines to examine the energy-linked functions of the kidney mitochondria in diabetic state or following insulin treatment [8,20].…”

“…oxidative energy metabolism is indeed altered in the diabetic state. Because there is a progressive damage to the kidneys as the disease advances [1][2][3][4][5][6][7][8][9][10][11], the effects were assessed at the end of 1 week and 1 month after the induction of the diabetic state following intraperitoneal injection of streptozotocin (STZ) to the rats. These are hereafter referred to as the early and the late effects.…”