Differential Response of Muscle and Gastric Histidine Decarboxylase to Compound 48/80 and Dietary Calcium

Greene, Steven M.; Fisher, Hans

doi:10.3181/00379727-180-42171

Cited by 5 publications

(1 citation statement)

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“…The skeletal muscle has also been shown to have a functioning PPP (Cabezas et al, 1999). In addition, there is evidence that other amino acids can be created or consumed by the skeletal muscle including arginine (Pardridge et al, 1982), histidine (Greene and Fisher, 1985), methionine (Scislowski et al, 1987), proline (Herzfeld et al, 1977), threonine (Scislowski and Davis, 1986), serine (Pardridge et al, 1982) and glycine (Kikuchi et al, 1980). This lit-erature evidence suggests that the skeletal muscle is able to transform 15 of the 20 common amino acids, with only tryptophan, tyrosine, phenylalanine, lysine, and cystine left unreacted.…”

Section: Metabolic Pathway Mapmentioning

confidence: 99%

Quantitative effects of thermal injury and insulin on the metabolism of the skeletal muscle using the perfused rat hindquarter preparation

Banta¹,

Yokoyama²,

Berthiaume³

et al. 2004

Biotech & Bioengineering

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Injury from a severe burn or trauma can propel the body into a hypermetabolic state that can lead to the significant erosion of lean muscle mass. Investigations describing this process have been somewhat limited due to the lack of adequate experimental models. Here we report the use of a perfused rat hindquarter preparation to study the consequences of a moderate burn injury (approximately 20% total body surface area), with or without the addition of exogenous insulin (12.5 mU/mL), on the fluxes of major metabolites across the isolated skeletal muscle. The metabolic flux data was further analyzed using metabolic flux analysis (MFA), which allows for the estimation of the impact of these conditions on the intracellular muscle metabolism. Results indicate that this model is able to capture the increased rate of proteolysis, glutamine formation, and the negative nitrogen balance associated with the burn-induced hypermetabolic state. The inclusion of exogenous insulin resulted in significant changes in several fluxes, including an increase in the metabolism of glucose and the flux through the pentose phosphate pathway, as well as a reduction in the metabolism of glutamine, alanine, and leucine. However, insulin administration did not affect the nitrogen balance or the rate of proteolysis in the muscle, as has been suggested using other techniques. The use of the perfused hindquarter model coupled with MFA is a physiologically relevant and experimentally flexible platform for the exploration of skeletal muscle metabolism under catabolic conditions, and it will be useful in quantifying the specific metabolic consequences of other therapeutic advances.

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