Differential response of pea (Pisum sativum L.) genotypes to iron deficiency in relation to the growth, rhizosphere acidification and ferric chelate reductase activities

Abstract

Calcareous soils are known problematic lands for agricultural systems because of the low availability of nutrients, particularly iron (Fe). The so-called strategy I plant (e. g. Pea, Pisum sativum L.) which groups dicotyledons and monocots other than grasses, developed root membrane activities that contribute to the improvement of Fe availability. Among the functions considered to be a critical phase in iron absorption is rhizosphere acidification by H-ATPase and Fe(III) reduced by Fe(III) chelate reducctase (FeCR). In order to experimentally investigate the importance of root FeCR in Fe nutrition, its relationship with rhizosphere acidification and the genotypic differences in response to iron deficiency in pea (Pisum sativum L.), a glasshouse experiment was conducted hydroponically on four genotypes Merveille de Kelvedon (MK); Lincoln (Lin); Douce de Provence (DP) and Alexandra (Alex). Plants of each genotype were distributed into two plots, the first one received full nutrient solution (+ Fe), the second one received nutrient solution devoid of iron (- Fe). Plant growth, Fe distribution, SPAD index and root acidification and ferric chelate reductase activities were evaluated. Fe deficiency decreased plant growth and SPAD index along with the significant increase of H-ATPase and FeCR activities. Some genotypic differences were observed as follows; Alex showed high tolerance to Fe deprivation as compared to other genotypes. Important H-ATPase and FeCR activities, high Fe use efficiency and adequate membrane efficiency are the main reasons for this tolerance. These physiological parameters could be used as tools of tolerance for further breeding programs

Genotypic differences in response of durum wheat (Triticum durum Desf.) to lime‐induced iron chlorosis

“…In agreement with our results, several studies showed that subjecting plants to direct Fe deficiency leads to a significant reduction of plant growth and chlorophyll accumulation (Ellsworth et al, 1997 ; Thoiron et al, 1997 ). Krouma ( 2021 ) showed in pea plants that the first consequence of a limited iron availability is the lack of chlorophyll, then young leaves yellowing. Karimi and Salimi ( 2021 ) demonstrated that the leaf chlorosis, electrolyte leakage, Mg and Ca increased significantly when plants are subjected to10 mM sodium bicarbonate, against a drastic decrease of leaf SPAD, N, K, active Fe, total Fe and Zn concentrations.…”

Functional Analysis of the Genotypic Differences in Response of Pea (Pisum sativum L.) to Calcareous-Induced Iron Deficiency

“…The primary symptom is interveinal chlorosis, the development of a yellow leaf with a network of dark green veins. It affects many agricultural crops in the calcareous soils of Tunisia [1]. In these soils having bicarbonate, high pH, and ferric form of iron, Fe deficiency is a major nutritional disorder for plants [1].…”

“…It affects many agricultural crops in the calcareous soils of Tunisia [1]. In these soils having bicarbonate, high pH, and ferric form of iron, Fe deficiency is a major nutritional disorder for plants [1]. These physicochemical conditions of soil affect the bioavailability of Fe and hamper plant growth and yield through the inhibition of Fe-dependent functions such as chlorophyll biosynthesis, photosynthesis, respiration, and protein formation [1].…”

“…In these soils having bicarbonate, high pH, and ferric form of iron, Fe deficiency is a major nutritional disorder for plants [1]. These physicochemical conditions of soil affect the bioavailability of Fe and hamper plant growth and yield through the inhibition of Fe-dependent functions such as chlorophyll biosynthesis, photosynthesis, respiration, and protein formation [1]. Ammari et al [2] indicated that measured Fe concentrations in calcareous soils exceed from a way the plant's demand and mentioned that 50%-90% of this Fe content was complexed by organic molecules.…”

“…Its main functions concern respiration, the synthesis of chlorophyll and photosynthesis [3]. The first consequence of limited iron availability is the lack of chlorophyll, followed by young leaf yellowing [1]. The function of various cytochromes containing heme Fe in the electron transport of photosynthesis is well established, as well as the role of ferredoxin.…”