Differential responses in gills of euryhaline tilapia, Oreochromis mossambicus, to various hyperosmotic shocks

Wang, Pei-Jen; Lin, Cheng Huang; Hwang, Lie-Yueh; Huang, Chao-Lu; Lee, Tsung‐Han; Hwang, Pung‐Pung

doi:10.1016/j.cbpa.2008.12.012

Cited by 43 publications

(37 citation statements)

References 57 publications

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“…2, 3). Although the reason for this discrepancy is not clear, it may suggest a complex regulation of the NKA system as previously reported by Lin et al (2003) and Wang et al (2009).…”

Section: Discussionmentioning

confidence: 45%

Monitoring of Na+/K+-ATPase mRNA expression in the cinnamon clownfish, Amphiprion melanopus, exposed to an osmotic stress environment: profiles on the effects of exogenous hormone

et al. 2011

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We examined changes in the expression of Na ? /K ? -ATPase mRNA in the gills of the cinnamon clownfish using quantitative real-time PCR in an osmotically changing environment [seawater (35 psu; practical salinity unit, 1 psu & 1%) ? brackish water (17.5 psu) and brackish water with prolactin]. The expression of Na ? / K ? -ATPase mRNA in gills was increased after the transfer to brackish water, and the expression was repressed by prolactin treatment. Also, activities of gill Na ? /K ? -ATPase and plasma cortisol levels increased after the transfer to brackish water and were repressed in brackish water with prolactin treatment. Na ? /K ? -ATPase-immunoreactive cells were almost consistently observed in the gill filaments, but absent from the lamella epithelia. The plasma osmolality level decreased in brackish water, but the level of this parameter increased in brackish water with prolactin treatment during salinity change. These results suggest that the Na ? /K ? -ATPase gene plays an important role in osmoregulation in gills, and prolactin improves the hyperosmoregulatory ability of cinnamon clownfish in a brackish water (hypoosmotic) environment.

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“…2, 3). Although the reason for this discrepancy is not clear, it may suggest a complex regulation of the NKA system as previously reported by Lin et al (2003) and Wang et al (2009).…”

Section: Discussionmentioning

confidence: 45%

Monitoring of Na+/K+-ATPase mRNA expression in the cinnamon clownfish, Amphiprion melanopus, exposed to an osmotic stress environment: profiles on the effects of exogenous hormone

et al. 2011

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“…into cells, important not only for sustaining intracellular homeostasis but also for providing a driving force for many transporting systems (Hwang and Lee 2007). Most studies on branchial NKA activity of tilapia revealed a pattern of expression proportional to environmental salinity (Wang et al 2009). The response of gill NKA to increased environmental salinity was measured at two levels; relative expression of the a1-subunit mRNA and absolute activity of this ion transporter.…”

Section: Discussionmentioning

confidence: 98%

Comparative time-course study on pituitary and branchial response to salinity challenge in Mozambique tilapia (Oreochromis mossambicus) and Nile tilapia (O. niloticus)

Velan

Hulata

Maymon

et al. 2011

Fish Physiol Biochem

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The physiological response of Mozambique and Nile tilapia transferred from fresh to brackish (15 ppt) water was compared during a one-week time course. Response in the pituitary was measured by the gene expression pattern of prolactin (PRL I), growth hormone (GH), and calcium-sensing receptor (CaSR), while the response in the gills was measured by the gene expression pattern of the prolactin receptor (PRL-R), Na(+)/K(+)/2Cl(-) cotransporter (NKCC) and Na(+)/Cl(-) cotransporter (NCC), and by activity and expression of Na(+)/K(+)-ATPase (NKA). The time-course curves of plasma osmolality levels indicate a rapid elevation 24 h after transfer, which later decreased and maintained at stable level. PRL I expression decreased in both species, but with stronger response in the Nile tilapia, while no differences were found in the slightly elevated levels of GH mRNA. The branchial response demonstrated a faster up-regulation of NKA and NKCC in the Mozambique tilapia, but similar levels after a week, while Nile tilapia had stronger and constant down-regulation of NCC. The time-course response of the measured osmoregulatory parameters indicate that 24 h after transfer is a critical time point for brackish-water adaptation. The differences in responses to saltwater challenge between Mozambique and Nile tilapia shown in this study may be associated with the differences in saltwater tolerance between these two tilapiine species.

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“…Tilapia are able to survive when transferred to an intermediate salinity before being transferred to SW, but are unable to survive a direct transfer (Yada et al, 1994;Seale et al, 2002Seale et al, , 2012bBreves et al, 2010d). It has been shown through many studies that the survival threshold is between 450 and 550 mOsmolal (Yada et al, 1994;Seale et al, 2002Seale et al, , 2012bKajimura et al, 2004;Wang et al, 2009;Breves et al, 2010c,d). Tilapia reared in a tidally changing salinity and then exposed to SW for 7 days showed no mortality, suggesting that they were completely acclimated to SW. We have observed previously that the plasma osmolality of tilapia reared in a tidally changing salinity fluctuated between 320 and 345 mOsmolal in the FW and SW phases of the cycle, respectively (Moorman et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

The effects of acute salinity challenges on osmoregulation in Mozambique tilapia reared in a tidally changing salinity

Moorman

Lerner

Grau

et al. 2015

Journal of Experimental Biology

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This study characterizes the differences in osmoregulatory capacity among Mozambique tilapia, Oreochromis mossambicus, reared in freshwater (FW), in seawater (SW) or under tidally driven changes in salinity. This was addressed through the use of an abrupt exposure to a change in salinity. We measured changes in: (1) plasma osmolality and prolactin (PRL) levels; (2) pituitary expression of prolactin (PRL) and its receptors, PRLR1 and PRLR2; (3) branchial expression of PRLR1, PRLR2, Na− co-transporter (NKCC), α1a and α1b isoforms of Na + /K + -ATPase (NKA), cystic fibrosis transmembrane conductance regulator (CFTR), aquaporin 3 (AQP3) and Na + /H + exchanger 3 (NHE3). Mozambique tilapia reared in a tidal environment successfully adapted to SW while fish reared in FW did not survive a transfer to SW beyond the 6 h sampling. With the exception of CFTR, the change in the expression of ion pumps, transporters and channels was more gradual in fish transferred from tidally changing salinities to SW than in fish transferred from FW to SW. Upon transfer to SW, the increase in CFTR expression was more robust in tidal fish than in FW fish. Tidal and SW fish successfully adapted when transferred to FW. These results suggest that Mozambique tilapia reared in a tidally changing salinity, a condition that more closely represents their natural history, gain an adaptive advantage compared with fish reared in FW when facing a hyperosmotic challenge.

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Differential responses in gills of euryhaline tilapia, Oreochromis mossambicus, to various hyperosmotic shocks

Cited by 43 publications

References 57 publications

Monitoring of Na+/K+-ATPase mRNA expression in the cinnamon clownfish, Amphiprion melanopus, exposed to an osmotic stress environment: profiles on the effects of exogenous hormone

Monitoring of Na+/K+-ATPase mRNA expression in the cinnamon clownfish, Amphiprion melanopus, exposed to an osmotic stress environment: profiles on the effects of exogenous hormone

Comparative time-course study on pituitary and branchial response to salinity challenge in Mozambique tilapia (Oreochromis mossambicus) and Nile tilapia (O. niloticus)

The effects of acute salinity challenges on osmoregulation in Mozambique tilapia reared in a tidally changing salinity

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