Differential responses of asthmatic airways to enantiomers of albuterol

Perrin‐Fayolle, M; Blum, Paul S.; Morley, John E.; Grosclaude, M.; Chambe, Marie-Thérèse

doi:10.1007/bf02772208

Cited by 44 publications

(25 citation statements)

References 33 publications

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“…An explanation for these observations may result from the potential adverse effects of (S)-albuterol found in the racemic product. (S)-albuterol has been shown to increase bronchial responsiveness to methacholine [34]and increase airway reactivity to histamine [35]. Furthermore, the metabolic clearance rate for (S)-albuterol is tenfold slower than the rate for (R)-albuterol [13, 14].…”

Section: Discussionmentioning

confidence: 99%

(S)-Albuterol Increases the Production of Histamine and IL-4 in Mast Cells

Cho

Hartleroad

2001

Int Arch Allergy Immunol

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Background: Racemic albuterol is an equimolar mixture of (R)-albuterol and (S)-albuterol. Previous studies indicated that (S)-albuterol may exert proinflammatory effects. We investigated the effect of (S)-albuterol in the production of mast cell mediators such as histamine and interleukin (IL)-4. Methods: Murine mast cells were either unstimulated or stimulated by IgE-receptor crosslinking. Both groups of mast cells were pretreated with either (R)- or (S)-albuterol. Histamine release and IL-4 secretion were measured by ELISA. Expression of L-histidine decarboxylase (L-HDC) and IL-4 was analyzed by semiquantitative reverse transcription-polymerase chain re- action. Results: In the overnight IgE-stimulated group, secreted histamine and total histamine were approximately 19.9 and 18.3% greater in cells co-treated with (S)-albuterol than untreated cells, respectively (n = 4, p < 0.002, p < 0.02), whereas there was no significant difference between the cells treated with (R)-albuterol and untreated cells. When the IgE-stimulated cells were treated with (S)-albuterol for 6 and 24 h, histamine release was approximately 18.3 and 24% greater, respectively (n = 4, p < 0.01). L-HDC is an essential enzyme for synthesizing histamine and its message was significantly induced in mast cells treated with (S)-albuterol. Both IL-4 message and protein were also significantly increased after treatment with (S)-albuterol. In the overnight IgE-stimulated group, IL-4 secretion was increased by approximately 58.8% upon exposure to (S)-albuterol (n = 5, p < 0.01). (R)-albuterol had no effect on mast cell mediator release. Conclusion: (S)-albuterol may have adverse effects in asthma control by activating mast cells to produce inflammatory mediators such as histamine and IL-4.

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Section: Discussionmentioning

confidence: 99%

(S)-Albuterol Increases the Production of Histamine and IL-4 in Mast Cells

Cho

Hartleroad

2001

Int Arch Allergy Immunol

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“…8 for many reasons including ease of administration. The pharmacokinetics of racemic salbutamol given Racemic salbutamol (albuterol) is one of the most orally and by IV routes have been studied previously widely used agents for treatment of asthma.…”

Section: Introductionmentioning

confidence: 99%

Pharmacokinetics and Pharmacodynamics of Cumulative Single Doses of Inhaled Salbutamol Enantiomers in Asthmatic Subjects

Gumbhir‐Shah

Kellerman

DeGraw

et al. 1999

Pulmonary Pharmacology & Therapeutics

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“…It is shown that intravenous infusion of the S enantiomers of isoproterenol and albuterol into guinea pigs induced airways hyperresponsiveness and studies conducted in patient with mild asthma have suggested that a deleterious effect for the S enantiomer of albuterol in human [32]. Furthermore, the fact that S enantiomer of albuterol sinificantly enhanced IL-5 induced superoxide production from human eosinophils suggests that S enantiomer of b-adrenoceptor agonist may have proVol.…”

Section: The Effect Of S1319 On Ige-mediated Protein Tyrosine Phosphomentioning

confidence: 99%