Differential responses of ectomycorrhizal fungi to heavy metals in vitro

Blaudez, Damien; Jacob, Christophe; Turnau, Katarzyna; Colpaert, Jan; Ahonen-Jonnarth, Ulla; Finlay, Roger D.; Botton, Bernard; Chalot, Michel

doi:10.1017/s0953756200003166

Cited by 130 publications

(101 citation statements)

References 22 publications

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“…Significant inter-and intraspecific variation in sensitivity to heavy metals by ECM fungi has been extensively reported Van Assche 1987, 1992;Denny and Wilkins 1987;Jones and Hutchinson 1988;EgertonWarburton and Griffin 1995;Hartley et al 1997;Blaudez et al 2000;Colpaert et al 2000). For example, the EC 50 (i.e., the effective concentration of metal that inhibits growth by 50%) values for Cd differed by over three orders of magnitude among the four ECM species studied by Hartley et al (1997).…”

Section: Introductionmentioning

confidence: 88%

“…For example, the EC 50 (i.e., the effective concentration of metal that inhibits growth by 50%) values for Cd differed by over three orders of magnitude among the four ECM species studied by Hartley et al (1997). In turn, Blaudez et al (2000) reported wide variation in Cd sensitivity among isolates of different ECM species, with EC 50 values of Suillus luteus isolates ranging from 0.04 to > 1 ppm. Several studies have compared the response of ECM isolates of the same species from contaminated and uncontaminated sites in an attempt to relate the degree of sensitivity to specific metals with the concentration of those metals in the soil of origin (Brown and Wilkins 1985;Denny and Wilkins 1987;Jones and Hutchinson 1988;Van Assche 1987, 1992;Egerton-Warburton and Griffin 1995;Blaudez et al 2000;Colpaert et al 2000).…”

Section: Introductionmentioning

confidence: 97%

“…Several studies have compared the response of ECM isolates of the same species from contaminated and uncontaminated sites in an attempt to relate the degree of sensitivity to specific metals with the concentration of those metals in the soil of origin (Brown and Wilkins 1985;Denny and Wilkins 1987;Jones and Hutchinson 1988;Van Assche 1987, 1992;Egerton-Warburton and Griffin 1995;Blaudez et al 2000;Colpaert et al 2000). Blaudez et al (2000) investigated the response of isolates of Paxillus involutus, Pisolithus tinctorius, Suillus bovinus, S. variegatus, and S. luteus to Cd, Co, Ni, and Zn and found no significant differences between EC 50 values of isolates from different soil categories (low, medium, or high metal concentration). By contrast, however, Colpaert et al (2000) reported that isolates of S. luteus from a site polluted with Zn and Cd were more tolerant to these metals than isolates from an unpolluted site.…”

Section: Introductionmentioning

confidence: 99%

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Genetic diversity and differential in vitro responses to Ni in Cenococcum geophilum isolates from serpentine soils in Portugal

et al. 2007

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Amplified fragment length polymorphism (AFLP) analysis was used to investigate the genetic diversity in isolates of the ectomycorrhizal fungus Cenococcum geophilum from serpentine and non-serpentine soils in Portugal. A high degree of genetic diversity was found among C. geophilum isolates; AFLP fingerprints showed that all the isolates were genetically distinct. We also assessed the in vitro Ni sensitivity in three serpentine isolates and one non-serpentine isolate. Only the nonserpentine isolate was significantly affected by the addition of Ni to the growth medium. At 30 μg g −1 Ni, radial growth rate and biomass accumulation decreased to 73.3 and 71.6% of control, respectively, a highly significant inhibitory effect. Nickel at this concentration had no significant inhibitory effect on serpentine isolates, and so the fitness of serpentine isolates, as evaluated by radial growth rate and biomass yield, is likely unaffected by Ni in the field. In all isolates, the Ni concentration in the mycelia increased with increasing Ni concentration in the growth medium, but two profiles of Ni accumulation were identified. One serpentine isolate showed a linear trend of Ni accumulation. At the highest Ni exposure, the concentration of Ni in the mycelium of this isolate was in the hyperaccumulation range for Ni as defined for higher plants. In the remaining isolates, Ni accumulation was less pronounced and seems to approach a plateau at 30 μg g −1 Ni. Because two profiles of Ni accumulation emerged among our Ni-insensitive serpentine isolates, this result suggests that different Ni detoxification pathways may be operating. The nonserpentine isolate whose growth was significantly affected by Ni was separated from the other isolates in the genetic analysis, suggesting a genetic basis for the Ni-sensitivity trait. This hypothesis is further supported by the fact that all isolates were maintained on medium without added Ni to avoid carry-over effects. However, because AFLP analysis failed to distinguish between serpentine and non-serpentine isolates, we cannot conclude that Ni insensitivity among our serpentine isolates is due to evolutionary adaptation. Screening a larger number of isolates, from different geographical origins and environments, should clarify the relationships between genetic diversity, morphology, and physiology in this important species.

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Section: Introductionmentioning

confidence: 88%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

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Genetic diversity and differential in vitro responses to Ni in Cenococcum geophilum isolates from serpentine soils in Portugal

et al. 2007

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“…Laccaria proxima appeared to be less sensitive to Ni, than Scleroderma avidum (Jones and Hutchinson 1988), and Suillus granulatus had a Cd-E50 value 1,500 times higher, than that of S. variegatus (Hartley et al 1997) and Suillus bovinus, S. luteus and S. variegatus isolates were strongly inhibited by Ni (Blaudez et al 2000).…”

Section: Accumulation Of Heavy Metals By Agaricus Bisporusmentioning

confidence: 84%

Growth responses and accumulation of heavy metals by fungus Agaricus bisporus

Mohammadhasani

Ahmadimoghadam

Asrar

et al. 2016

Acta Botanica Hungarica

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Ectomycorrhizal fungi are able to form symbiotic associations with tree roots, and therefore, plants gain different benefits. On metal-contaminated soils, these fungi may improve plant fitness through an enhanced nutrition or by reducing toxicity of the metals. Agaricus bisporus, an edible fungus has been noted to grow in large numbers under Pistacia vera plantations in orchards of Kerman, Iran, indicating that it may form ectomycorrhiza with the tree. This research describes the responses of this fungus to heavy metals in solid and liquid MMN media. The fungus was grown in vitro in liquid and solid cultures for 3 weeks on five different concentrations (0, 15, 30, 45, 60 ppm) of five heavy metals (Cu, Zn, Ni, Co, Mn) as sulphate and the effect of these metal on radial growth, biomass production and metal content of fungal biomass were determined. The result showed there was a strong variation in metal tolerance, so that Agaricus bisporus was more tolerant to Mn than other metals, while the reverse was true for Ni, so that the fungus had an increased growth in the presence of low concentrations of Co, Mn, and Zn, but Ni greatly inhibited increase in biomass and colony diameter even at concentrations as low as 15 mg/l.

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“…Through extracellular mechanisms, fungi can prevent Cr(VI) input to the cell, and these include i) uptake of reduced Cr(VI) or increasing efflux of metal; ii) immobilisation of Cr(VI) by adsorption on the cell wall, or extracellular precipitation by neoformed secondary minerals; and iii) extracellular sequestration of Cr(VI) by exopolysaccharides and other extracellular metabolites (Gadd 1993a;Macreadie et al 1994;Blaudez et al 2000;Perotto et al 2002;Baldrian 2003).…”

Section: Extracellular Mechanismsmentioning

confidence: 99%

Metallophilic fungi research: an alternative for its use in the bioremediation of hexavalent chromium

García-Hernández

Villarreal-Chiu

Garza-González

2017

Int. J. Environ. Sci. Technol.

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Contamination by hexavalent chromium has had a large impact on modern society and human health. This problem is a consequence of its great industrial applicability to several products and processes. Short-term exposure to hexavalent chromium can cause irritation, ulceration in skin and stomach and in addition to cancer, dermatitis, and damage to liver, renal circulation and nervous tissues, with even death being observed in response to long-term exposures. Many techniques have been used for the remediation of this pollutant, including physical and chemical approaches and, in more recent years, biological methods. Filamentous fungi isolated from contaminated sites exhibit a significant tolerance to heavy metal; hence, they are an important source of microbiota capable of eliminating hexavalent chromium from the environment. However, these microorganisms can do so in different ways, including biosorption, bioreduction, and bioaccumulation, among others. In this review, we explore several of the most documented mechanisms that have been described for fungi/hexavalent chromium interactions and their potential use in bioremediation.

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Differential responses of ectomycorrhizal fungi to heavy metals in vitro

Cited by 130 publications

References 22 publications

Genetic diversity and differential in vitro responses to Ni in Cenococcum geophilum isolates from serpentine soils in Portugal

Genetic diversity and differential in vitro responses to Ni in Cenococcum geophilum isolates from serpentine soils in Portugal

Growth responses and accumulation of heavy metals by fungus Agaricus bisporus

Metallophilic fungi research: an alternative for its use in the bioremediation of hexavalent chromium

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