Differential responses of root growth, acid invertase activity and transcript level to copper stress in two contrasting populations of Elsholtzia haichowensis

Cai, Shenwen; Xiong, Zhuang; Li, Ling; Li, Minjing; Zhang, Luan; Liu, Chen; Xu, Zhong-Rui

doi:10.1007/s10646-013-1153-y

Cited by 16 publications

(17 citation statements)

References 53 publications

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“…Excess Cu ions in plants cause disturbances in photosynthesis, which can result in oxidative stress, with increasing reactive oxygen species (ROS), reducing carbon fixation, and reducing or inhibiting plant development (Küpper and Andresen 2016). Exposure to Cu stress leads to remodeling in root morphology (Cai et al 2014), since the root is the first organ to come into contact with the contaminant. The use of digital images of roots is an important parameter for analysis of root development.…”

Section: Introductionmentioning

confidence: 99%

Copper Toxicity on Photosynthetic Responses and Root Morphology of Hymenaea courbaril L. (Caesalpinioideae)

Marques

Júnior

Silva

et al. 2018

Water Air Soil Pollut

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Copper (Cu) is a micronutrient essential for plant development. However, in excess, it is toxic to plants and may cause various physiological and morphological changes. The study of the growth of plants exposed to excess Cu is important for the development of phytoremediation programs and for understanding the mechanisms involved in the tolerance of this metal. In this context, the objective of this research was to evaluate the effect of excess copper on photosynthetic responses and root morphology of Hymenaea courbaril L. Biometric measurements, gas exchange, root morphology, and Cu content in tissues and indices (TI and TF) were assessed, involving metal content and biomass. Up to a concentration of 200 mg kg −1 , Cu favored growth, gas exchange, and root morphology of the plants under study. At a higher concentration (800 mg kg −1) in the soil, it affected plant growth and caused a decrease in photosynthetic rate. Biochemical limitations in photosynthesis were observed, as well as lower maximum net photosynthetic rate (A max), respiration rate in the dark (R d), light compensation point (LCP), light saturation point (LSP), and apparent quantum yield (α), when exposed to excess Cu. Root length, surface area, mean diameter, root volume, dry biomass, and specific root length decreased with high Cu concentrations in the soil. Cu was accumulated in the roots as a mechanism of tolerance to the excess of this metal in order to preserve the most metabolically active tissues present in the leaves. At a concentration of 800 mg kg −1 , copper also caused inhibition of the root system. Plants of H. courbaril showed tolerance to excess Cu in the soil and can be indicated for the recovery of areas contaminated with this metal.

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Section: Introductionmentioning

confidence: 99%

Copper Toxicity on Photosynthetic Responses and Root Morphology of Hymenaea courbaril L. (Caesalpinioideae)

Marques

Júnior

Silva

et al. 2018

Water Air Soil Pollut

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“…The optimum temperature of GspInv was 45 • C. More than 50% of the protein activity was shown when tested at temperatures between 30 and 55 • C (Figure 3B). The optimum temperature and pH of GspInv were similar to most of the invertases from other fungi, with optimum temperature and pH of 40-70 • C and 4.5-6.5, respectively (Acosta et al, 2000;Cai et al, 2014;Nadeem et al, 2015).…”

Section: Gspinv Characterizationmentioning

confidence: 64%

Identification and Immobilization of an Invertase With High Specific Activity and Sucrose Tolerance Ability of Gongronella sp. w5 for High Fructose Syrup Preparation

et al. 2020

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Invertases catalyze the hydrolysis of sucrose into fructose and glucose and can be employed as an alternative in producing high fructose syrup. In this study, we reported the heterologous expression of an invertase gene (GspInv) of Gongronella sp. w5 in Komagataella pastoris. GspInv activity reached 147.6 ± 0.4 U/mL after 5 days of methanol induction. GspInv is invertase with a high specific activity of 2,776.1 ± 124.2 U/mg toward sucrose. GspInv showed high tolerance to sucrose (IC 50 = 1.2 M), glucose (IC 50 > 2 M), fructose (IC 50 = 1.5 M), and a variety of metal ions that make it an ideal candidate for high fructose syrup production. A carbohydratebinding module was sequence-optimized and fused to the N-terminus of GspInv. The fusion protein had the highest immobilization efficiency at room temperature within 1 h adsorption, with 1 g of cellulose absorption up to 8,000 U protein. The celluloseimmobilized fusion protein retained the unique properties of GspInv. When applied in high fructose syrup preparation by using 1 M sucrose as the substrate, the sucrose conversion efficiency of the fused protein remained at approximately 95% after 50 h of continuous hydrolysis on a packed bed reactor. The fused protein can also hydrolyze completely the sucrose in sugarcane molasses. Our results suggest that GspInv is an unusual invertase and a promising candidate for high fructose syrup preparation.

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“…E. haichowensis was proven to possess the exclusion strategy, comprising avoidance of metal uptake and restriction of metal transport to the shoots (Lou et al 2004;Song et al 2004). Its population from mine accumulated significantly lower Cu than that from none contaminated sites under Cu stress, thus possibly reducing the Cu exposure of invertases (Ke et al 2008;Cai et al 2014). Excess metal ions can be removed from the cytosol by efflux or compartmentation, which further reduce the Cu exposure of invertase.…”

Section: Discussionmentioning

confidence: 99%

“…It is proposed that acid invertases play a vital role in regulation of Suc transport and partitioning, sugar storage, cell differentiation and enlargement, as well as the response to various biotic and abiotic stresses such as wounding, cold, drought, pathogen infection, salinity and hypoxia (Sturm and Tang 1999;Roitsch and González 2004;Ruan et al 2010). Moreover, recent studies have shown that acid invertases were crucial for metallophyte plants to govern root growth and root/shoot biomass allocation, and at least partly associated with Cu tolerance by supplying carbon and energy for tolerance mechanisms (Stobrawa and LorencPlucińska 2007;Xiong et al 2008;Huang et al 2011;Cai et al 2013Cai et al , 2014.…”

Section: Introductionmentioning

confidence: 99%

“…Similar results were obtained from other metallophytes such as Rumex dentatus (Cai et al 2013), Rumex japonicas (Huang et al 2011) and Kummerowia stipulacea . Several possible mechanisms were proposed to explain the higher acid invertase activities in tolerant populations, such as point mutations in invertase genes, up-regulation of expression of invertase genes under Cu stress and protection of invertases from deactivation by tolerance mechanisms Cai et al 2014).…”

Section: Introductionmentioning

confidence: 99%

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Heterologous expression and comparative characterization of vacuolar invertases from Cu-tolerant and non-tolerant populations of Elsholtzia haichowensis

Liu

Cai

et al. 2015

Plant Cell Rep

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Vacuolar invertases (VINs) from Cu-tolerant and non-tolerant populations of Elsholtzia haichowensis have similar enzyme properties, and the enzyme protein divergences contribute little to the varied VIN activities between the contrasting populations. In our previous studies of Elsholtzia haichowensis, vacuolar invertase (VIN) activity in roots of a Cu-tolerant population was found to be significantly higher than that of a non-tolerant population under Cu stress. Divergences of amino acid residues in a sucrose-binding box and other regions of the VINs were detected. To test whether the amino acid divergences influence the enzyme properties of VINs, and thus are relevant to the differences in enzyme activities between the contrasting populations of E. haichowensis, two VIN genes from the Cu-tolerant population (EhCvINV) and non-tolerant population (EhNvINV) were heterologously expressed in Pichia pastoris, and the enzyme properties of the recombinants were characterized and compared. Both of the recombinant enzymes showed temperature optima of 70 °C and pH optima of 4.5-5.5. Copper as well as other heavy metals caused almost the same inhibition to EhNvINV and EhCvINV. No statistically significant differences were observed between EhNvINV and EhCvINV in K m and k cat values for sucrose. The results provided evidence that the observed residue divergences had little influence on the enzyme properties of VIN in E. haichowensis, and the varied VIN activities between the contrasting populations under Cu stress were not relevant to the amino acid divergences in the proteins. Also, some other possible reasons accounting for this difference in invertase activities were discussed, such as up-regulation of expression of the EhCvINV gene under Cu stress, as Cu tolerance mechanisms in Cu-mine plants.

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Differential responses of root growth, acid invertase activity and transcript level to copper stress in two contrasting populations of Elsholtzia haichowensis

Cited by 16 publications

References 53 publications

Copper Toxicity on Photosynthetic Responses and Root Morphology of Hymenaea courbaril L. (Caesalpinioideae)

Copper Toxicity on Photosynthetic Responses and Root Morphology of Hymenaea courbaril L. (Caesalpinioideae)

Identification and Immobilization of an Invertase With High Specific Activity and Sucrose Tolerance Ability of Gongronella sp. w5 for High Fructose Syrup Preparation

Heterologous expression and comparative characterization of vacuolar invertases from Cu-tolerant and non-tolerant populations of Elsholtzia haichowensis

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