Differential responses of three sweetpotato metallothionein genes to abiotic stress and heavy metals

Kim, Sun Ha; Jeong, Jae Cheol; Ahn, Young Ock; Lee, Haeng Soon; Kwak, Sang Soo

doi:10.1007/s11033-014-3582-y

Cited by 29 publications

(10 citation statements)

References 34 publications

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“…Our analysis of conserved miRNAs confirms their response to osmotic stress and variation among tissue types. The grapespecific miR3624 was inhibited by drought, while its target gene, encoding a metal ion-binding protein, increased greatly, in accordance with the known up-regulation of metallothionein-induced abiotic stress (Kim et al, 2014). miR393 positively responded to drought exclusively in the roots of the tested genotypes.…”

Section: Conserved and Novel Grapevine Mirnas Respond To Ds In The Twsupporting

confidence: 59%

The Accumulation of miRNAs Differentially Modulated by Drought Stress Is Affected by Grafting in Grapevine

et al. 2017

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Grapevine (Vitis vinifera) is routinely grafted, and rootstocks inducing drought tolerance represent a source for adapting vineyards to climate change in temperate areas. Our goal was to investigate drought stress effects on microRNA (miRNA) abundance in a drought-resistant grapevine rootstock, M4 (Vitis vinifera 3 Vitis berlandieri), compared with a commercial cultivar, Cabernet Sauvignon, using their autografts and reciprocal grafts. RNA extracted from roots and leaves of droughted and irrigated plants of different graft combinations was used to prepare cDNA libraries for small RNA sequencing and to analyze miRNAs by quantitative real-time polymerase chain reaction (RT-qPCR). Measurements of leaf water potential, leaf gas exchange, and root hydraulic conductance attested that, under irrigation, M4 reduced water loss in comparison with cultivar Cabernet Sauvignon mostly through nonhydraulic, root-specific mechanisms. Under drought, stomatal conductance decreased at similar levels in the two genotypes. Small RNA sequencing allowed the identification of 70 conserved miRNAs and the prediction of 28 novel miRNAs. Different accumulation trends of miRNAs, observed upon drought and in different genotypes and organs, were confirmed by RT-qPCR. Corresponding target transcripts, predicted in silico and validated by RT-qPCR, often showed opposite expression profiles than the related miRNAs. Drought effects on miRNA abundance differed between the two genotypes. Furthermore, the concentration of drought-responsive miRNAs in each genotype was affected by reciprocal grafting, suggesting either the movement of signals inducing miRNA expression in the graft partner or, possibly, miRNA transport between scion and rootstock. These results open new perspectives in the selection of rootstocks for improving grapevine adaptation to drought.

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Section: Conserved and Novel Grapevine Mirnas Respond To Ds In The Twsupporting

confidence: 59%

The Accumulation of miRNAs Differentially Modulated by Drought Stress Is Affected by Grafting in Grapevine

et al. 2017

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“…To investigate the expression of IbPsbP, 1-month-old sweetpotato plants with six fully expanded leaves were subjected to various stresses [38 and 47°C (heat), 4°C (cold), 100 mM methyl viologen (MV), 10 mM H 2 O 2 , and 200 mM NaCl] (Kim et al, 2014; Park et al, 2016), and then samples were taken from the fully expanded leaves third from the top of treated plants at the indicated time. Two-month-old sweetpotato plants were used for acquisition of samples of leaves from shoot apical meristem, according to position-dependent development stage (Ji et al, 2016).…”

Section: Methodsmentioning

confidence: 99%

IbOr Regulates Photosynthesis under Heat Stress by Stabilizing IbPsbP in Sweetpotato

et al. 2017

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The Orange (Or) protein regulates carotenoid biosynthesis and environmental stress in plants. Previously, we reported that overexpression of the sweetpotato [Ipomoea batatas (L.) Lam] Or gene (IbOr) in transgenic Arabidopsis (referred to as IbOr-OX/At) increased the efficiency of photosystem II (PSII) and chlorophyll content after heat shock. However, little is known about the role of IbOr in PSII-mediated protection against abiotic stress. In this study, comparative proteomics revealed that expression of PsbP (an extrinsic subunit of PSII) is up-regulated in heat-treated IbOr-OX/At plants. We then identified and functionally characterized the PsbP-like gene (IbPsbP) from sweetpotato. IbPsbP is predominantly localized in chloroplast, and its transcripts are tissue-specifically expressed and up-regulated in response to abiotic stress. In addition, IbOr interacts with IbPsbP and protects it from heat-induced denaturation, consistent with the observation that transgenic sweetpotato overexpressing IbOr maintained higher PSII efficiency and chlorophyll content upon exposure to heat stress. These results indicate that IbOr can protect plants from environmental stress not only by controlling carotenoid biosynthesis but also by directly stabilizing PSII.

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“…Moreover, co-application of 24-epibrassinolide (EBL) and salicylic acid (SA) resulted in the improvement of root and shoot lengths and chlorophyll content, which can regulate anti-oxidative defense responses and gene expression in Brassica juncea L. seedlings under Pb stress (Kohli et al, 2018). Metallothioneins (MTs) that are cysteine-rich, of low molecular weight, metal-binding proteins can enhance tolerance to heavy metals through differential responses in sweet potato (Kim et al, 2014), which may be strongly associated with Cd uptake, but not related to Cd transport from root to leaf, nor Cd enrichment in shoots (Li et al, 2018). Despite striking advances in gene function research involved with response or transport heavy metals, identifying novel accurate biomarkers to detect environmental pollutants is urgently needed.…”

Section: Introductionmentioning

confidence: 99%

Integrated bioinformatics analysis of As, Au, Cd, Pb and Cu heavy metal responsive marker genes through Arabidopsis thaliana GEO datasets

Niu

Jiang

et al. 2019

PeerJ

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Background Current environmental pollution factors, particularly the distribution and diffusion of heavy metals in soil and water, are a high risk to local environments and humans. Despite striking advances in methods to detect contaminants by a variety of chemical and physical solutions, these methods have inherent limitations such as small dimensions and very low coverage. Therefore, identifying novel contaminant biomarkers are urgently needed. Methods To better track heavy metal contaminations in soil and water, integrated bioinformatics analysis to identify biomarkers of relevant heavy metal, such as As, Cd, Pb and Cu, is a suitable method for long-term and large-scale surveys of such heavy metal pollutants. Subsequently, the accuracy and stability of the results screened were experimentally validated by quantitative PCR experiment. Results We obtained 168 differentially expressed genes (DEGs) which contained 59 up-regulated genes and 109 down-regulated genes through comparative bioinformatics analyses. Subsequently, the gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichments of these DEGs were performed, respectively. GO analyses found that these DEGs were mainly related to responses to chemicals, responses to stimulus, responses to stress, responses to abiotic stimulus, and so on. KEGG pathway analyses of DEGs were mainly involved in the protein degradation process and other biologic process, such as the phenylpropanoid biosynthesis pathways and nitrogen metabolism. Moreover, we also speculated that nine candidate core biomarker genes (namely, NILR1, PGPS1, WRKY33, BCS1, AR781, CYP81D8, NR1, EAP1 and MYB15) might be tightly correlated with the response or transport of heavy metals. Finally, experimental results displayed that these genes had the same expression trend response to different stresses as mentioned above (Cd, Pb and Cu) and no mentioned above (Zn and Cr). Conclusion In general, the identified biomarker genes could help us understand the potential molecular mechanisms or signaling pathways responsive to heavy metal stress in plants, and could be applied as marker genes to track heavy metal pollution in soil and water through detecting their expression in plants growing in those environments.

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Differential responses of three sweetpotato metallothionein genes to abiotic stress and heavy metals

Cited by 29 publications

References 34 publications

The Accumulation of miRNAs Differentially Modulated by Drought Stress Is Affected by Grafting in Grapevine

The Accumulation of miRNAs Differentially Modulated by Drought Stress Is Affected by Grafting in Grapevine

IbOr Regulates Photosynthesis under Heat Stress by Stabilizing IbPsbP in Sweetpotato

Integrated bioinformatics analysis of As, Au, Cd, Pb and Cu heavy metal responsive marker genes through Arabidopsis thaliana GEO datasets

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