Differential responses of two rice varieties to salt stress

Ghosh, Nirmalya; Adak, Malay Kumar; Ghosh, Parthadeb; Gupta, S. Dutta; Gupta, Dolly; Mandal, Chiranjib

doi:10.1007/s11816-010-0163-y

Cited by 79 publications

(42 citation statements)

References 41 publications

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“…Then assay of O 2 − was done as described by Achary et al (2007). For detection of H 2 O 2, concentration of the tissue was done according to Ghosh et al (2011). Fresh leaf tissue of 0.5 g was thoroughly homogenized in 3 ml of 1 % (w/v) trichloro acetic acid (TCA).…”

Section: Plant Materialsmentioning

confidence: 99%

“…guaiacol peroxidase (GPX), ascorbate peroxidase (APX), catalase (CAT), glutathione reductase (GR) and super oxide dismutase (SOD) were studied following appropriate protocol for extraction, purification and assay reaction according to Ghosh et al (2011). For each type of enzyme 1.0 g of tissue was taken and crushed in liquid nitrogen followed by homogenization with extraction buffer (3.0 ml) containing 1.0 mM Tris-HCl (pH 7.7), 10 mM MgCl 2 , 1.0 mM DTT, 0.1 mM PMSF, 0.1 mM EDTA, 0.1 mM leupeptin, 0.1 mM BSA and 2 % PVP under cold condition.…”

Section: Estimation Of Glutathione Contentmentioning

confidence: 99%

“…The change of absorbance was monitored at 430 nm. The enzyme activity was determined according to Ghosh et al (2011) using an extinction coefficient of Odianisidine (26.2 mM −1 cm −1 ). One unit (U) of enzyme is defined, as the time required for changing the absorbance by 0.1 per unit gram of protein.…”

Section: Estimation Of Glutathione Contentmentioning

confidence: 99%

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Antioxidative responses of Salvinia (Salvinia natans Linn.) to aluminium stress and it’s modulation by polyamine

Mandal

Ghosh

Maiti

et al. 2012

Physiol Mol Biol Plants

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Antioxidative stress response of free-floating aquatic fern (Salvinia natans Linn.) was studied under increasing toxic amount of aluminium (Al) and its modulation by exogenous application of polymaine. Increased levels of superoxide (O 2 − ) and hydrogen peroxide (H 2 O 2 ) species from affected tissues suggested that plants were undergoing oxidative stress and it was concominant with increased accumulation of Al in a dose dependent manner. Application of polyamine like putrescine (Put) led to a decrease in oxidative stress as revealed by reduced level of O 2 − and H 2 O 2 . Al toxicity resulted into decreased biomass that was ameliorated by the application of Put. The changes observed in lipid peroxidation (MDA) and protein oxidation also indicated that plats are undergoing Al induced oxidative stress. In order to circumvent the oxidative stress resulting from Al toxicity, plants enzymatic and nonenzymatic antioxidant pathways were active. The ratio of both oxidized and reduced cellular glutathione exhibited significant variation in response to Al stress and was improved upon Put treatment. Peroxidase and glutathione were upregulated whereas catalse was downregulated under varying doses of Al. Isozyme profile of above enzymes also showed a trend with increasing amount of Al. The nuclear disintegration study using comet assay was indicative of Al induced oxidative stress. In the present study, we have explored the antioxidative response of aquatic fern Salvinia natans Linn in response to Al toxicity. The application of polyamine Put improved the overall antioxidative response and thus would make it a better candidate to be used as hyper accumulator of Al and other toxic metals.

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Section: Plant Materialsmentioning

confidence: 99%

Section: Estimation Of Glutathione Contentmentioning

confidence: 99%

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Antioxidative responses of Salvinia (Salvinia natans Linn.) to aluminium stress and it’s modulation by polyamine

Mandal

Ghosh

Maiti

et al. 2012

Physiol Mol Biol Plants

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“…The generation of H 2 O 2 in plant cells is present in a number of cellular metabolic pathways, including photosynthesis, photorespiration and other processes involving the electron transfer in cellular organelle. Under abiotic stress, ROS generation is almost mandatory (Ghosh et al 2011). By comparing with superoxide (O 2 •), hydroxyl radical (•OH) and other ROS, H 2 O 2 is the most stable compound (Cheeseman 2007).…”

Section: Introductionmentioning

confidence: 99%

Interaction of polyamine on oxidative stress induced by exogenously applied hydrogen peroxide in Salvinia natans Linn

Mandal¹,

Ghosh²,

Adak³

et al. 2013

Theor. Exp. Plant Physiol.

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A hydroponics experiment was carried out to study the role of hydrogen peroxide (H 2 O 2 ) through changes of oxidative stress and antioxidation activity in Salvinia natans Linn. H 2 O 2 is an active oxygen species, widely generated in many biological systems, and mediates various physiological and biochemical processes in plants. In this study, we demonstrated that exogenous H 2 O 2 causes the degradation of pigments and induces the oxidative stress and the antioxidation activity. These effects enhanced with increments of applied H 2 O 2 concentration. Application of polyamine reduced the generation and the accumulation of H 2 O 2 and modulated the enzyme activities.

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“…H2O2 content of treated plants was done according to Ghosh et al (2011) (16). 500 mg of plant tissue was homogenized in liquid nitrogen by adding 2 ml of 1 % (w/v) trichloro acetic acid (TCA).…”

Section: Hydrogen Peroxide (H2o2) Contentmentioning

confidence: 99%

Physiological characterization of SUB1 trait in rice under subsequent submergence and re-aeration with interaction of chemical elicitors

Sarkar

Adak

2017

Plant Sci. Today

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Abstract:In the present study, the sensitivity of two chemical elicitors: polyamine (PA) and salicylic (SA) acid were exercised for submergence sensitivity in Swarna Sub1A rice variety. Under 5 days of submergence, the antioxidation responses were more distinguished in plants against control. Along with the anti-oxidation modules, significant changes in biomolecule loss were registered through lipid and protein oxidation by 1.91 and 1.46 -fold respectively. PA and SA treated plants were the reliever to recover the membrane potential. Total carbohydrate and reducing sugars were varied under submergence by down regulation of 36.66 and 44.44% as compared to control. This was also supported by regulation of α-amylase activity under submergence that also recovered significantly with PA and SA treatments against submergence. In association with carbohydrate metabolism, Under submergence Swarna Sub1A recorded to be prone with oxidative stress through O 2. -(1.55 fold) and peroxide (1.70-fold) over-accumulation but recovered as PA and SA applied. In both cases, sustenance of non-enzymatic anti-oxidant like total carotenoid and lycopene content were also contributory through down-regulation. The enzymatic anti-oxidation paths like SOD, GPX, CAT and GR were regulated by 11.11, 19.54, 13.65, 10.03% declined respectively and thoroughly discussed with reference to PA and SA interactions.

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Differential responses of two rice varieties to salt stress

Cited by 79 publications

References 41 publications

Antioxidative responses of Salvinia (Salvinia natans Linn.) to aluminium stress and it’s modulation by polyamine

Antioxidative responses of Salvinia (Salvinia natans Linn.) to aluminium stress and it’s modulation by polyamine

Interaction of polyamine on oxidative stress induced by exogenously applied hydrogen peroxide in Salvinia natans Linn

Physiological characterization of SUB1 trait in rice under subsequent submergence and re-aeration with interaction of chemical elicitors

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