Differential roles of the phospholipase C genes in fungal development and pathogenicity of Magnaporthe oryzae

Genetic control of infection-related development in Magnaporthe oryzae

“…Systematic characterization of proteins related to calcium signaling, including the MoCch1 Ca 2+ channel, calmodulin, and PMC1 Ca 2+ pump, confirmed its role in infection-related morphogenesis in M. oryzae [28]. The MoPLC1, MoPLC2, and MoPLC3 phospholipase C genes and both catalytic (MCNA) and regulatory (MCNB) subunits of calcineurin also have been functionally characterized in M. oryzae [29]. …”

Expression of the Cameleon calcium biosensor in fungi reveals distinct Ca2+ signatures associated with polarized growth, development, and pathogenesis

“…M. oryzae produces one such structure called the appressorium to breach the host epidermal layer (Howard and Valent, 1996). Gene functional studies suggested the involvement of Ca 2+ signaling in M. oryzae appressorium formation (Choi et al, 2011;Nguyen et al, 2008). We imaged [Ca 2+ ] c changes during appressorial formation both in vitro and in planta.…”

“…The Ca 2+ signaling pathway has been shown to control spore production and germination (Choi et al, 2011;Shaw and Hoch, 2001), hyphal growth and differentiation (Brand et al, 2009;Chen et al, 2011), sexual development (Cavinder et al, 2011;Hallen et al, 2007), circadian rhythm (Yang et al, 2001), toxin biosynthesis (Chung, 2003), appressorium formation (Choi et al, 2011(Choi et al, , 2009, and pathogenesis in both plants and animals (Bowman et al, 2009;Nguyen et al, 2008;Steinbach et al, 2006). Rapidly increasing fungal genome sequences have facilitated systematic identification of genes involved in Ca 2+ signaling (Nguyen et al, 2008;Rispail et al, 2009;Zelter et al, 2004), and the functions of some of these genes have been characterized via targeted mutagenesis (Choi et al, 2011;Nguyen et al, 2008;Soriani et al, 2008).…”

“…Rapidly increasing fungal genome sequences have facilitated systematic identification of genes involved in Ca 2+ signaling (Nguyen et al, 2008;Rispail et al, 2009;Zelter et al, 2004), and the functions of some of these genes have been characterized via targeted mutagenesis (Choi et al, 2011;Nguyen et al, 2008;Soriani et al, 2008). However, a major void in fungal Ca 2+ signaling mechanisms exists, because visualization of [Ca 2+ ] c dynamics in fungal cells at the single cell level has been hampered by a number of significant technical challenges.…”

Multiple cellular roles of Neurospora crassa plc-1, splA2, and cpe-1 in regulation of cytosolic free calcium, carotenoid accumulation, stress responses, and acquisition of thermotolerance

“…In Magnaporthe oryzae, the rice blast fungus, the MoPLC1 gene encodes a fungal PI-PLC-δ isoform that regulates intracellular Ca 2+ fluxes essential for fungal development, appressorium formation and pathogenicity (Rho et al, 2009). Moreover, two other PLC isozymes MoPLC2 and MoPLC3 exhibit, respectively, 58% and 49% identity to the PLC1 gene of N. crassa (NCU06245), and play distinct roles in M. oryzae including regulating development and appres- (Choi et al, 2011). In Alternaria alternata, the citrus fungal pathogen, the PLC1 homologue plays an important role in vegetative growth, conidial formation, Ca 2+ homeostasis, and virulence (Tsai and Chung, 2014).…”