Differential scanning calorimetry and temperature dependence of electric conductivity in studies on denaturation process of bone collagen

Kubisz, Leszek; Mielcarek, S.

doi:10.1016/j.jnoncrysol.2005.05.041

Cited by 23 publications

(17 citation statements)

References 38 publications

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“…Upon heating to 100-150 °C, bone is progressively dehydrated [15] and collagen is considered to be fully degraded at ~ 400 °C [16,17]. Most X-ray studies concluded an absence of mineral crystal structure modifications before 400 °C, while a rapid crystal growth has been reported at ~ 750 °C [18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Ultrafine heat-induced structural perturbations of bone mineral at the individual nanocrystal level

et al. 2018

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To cite this version:M. Verezhak, E.F. Rauch, M. Veron, C. Lancelon-Pin, J.-L. Putaux, et al.. Ultrafine heat-induced structural perturbations of bone mineral at the individual nanocrystal level. Acta Biomaterialia, Elsevier, 2018Elsevier, , 10.1016Elsevier, /j.actbio.2018 Online version: https://www.sciencedirect.com/science/article/pii/S1742706118301946 Abstract: The nanoscale characteristics of the mineral phase in bone tissue such as nanocrystal size, organization, structure and composition have been identified as potential markers of bone quality. However, such characterization remains challenging since it requires combining structural analysis and imaging modalities with nanoscale precision. In this paper, we report the first application of automated crystal orientation mapping using transmission electron microscopy (ACOM-TEM) to the structural analysis of bone mineral at the individual nanocrystal level. By controlling the nanocrystal growth of a cortical bovine bone model artificially heated up to 1000 ºC, we highlight the potential of this technique. We thus show that the combination of sample mapping by scanning and the crystallographic information derived from the collected electron diffraction patterns provides a more rigorous analysis of the mineral nanostructure than standard TEM. In particular, we demonstrate that nanocrystal orientation maps provide valuable information for dimensional analysis. Furthermore, we show that ACOM-TEM has sufficient sensitivity to distinguish between phases with close crystal structures and we address unresolved questions regarding the existence of a hexagonal to monoclinic phase transition induced by heating. This first study therefore opens new perspectives in bone characterization at the nanoscale, a daunting challenge in the biomedical and archaeological fields, which could also prove particularly useful to study the mineral characteristics of tissue grown at the interface with biomaterials implants.

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Section: Introductionmentioning

confidence: 99%

Ultrafine heat-induced structural perturbations of bone mineral at the individual nanocrystal level

et al. 2018

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“…The peak is the result of thermal denaturation of collagen which is the main component of bone protein matrix [9]. By analogy with bone, the peak observed for the coral material at 203°C, is the result of the thermal denaturation of the dry protein matrix.…”

Section: Discussionmentioning

confidence: 96%

Temperature dependence of electric conductivity of bamboo coral skeleton and glass sponge spicules, the marine origin biomaterials

Kubisz

Ehrlich

2007

Journal of Non-Crystalline Solids

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“…The range of denaturation temperatures is an outcome of the degree of collagen crystallinity, water content and the presence of other substances such as hydroxyapatite (HAP). Usually denaturation of collagen is observed in the temperature range of 450-520 K, but denaturation of bone collagen was also found at 428 K [3,10,21,22]. Changes in the thermal stability of collagen can be shown either by an increase or a decrease in the temperature of denaturation [23][24][25][26].…”

Section: Studies On Thermal Denaturation Of Proteinsmentioning

confidence: 99%

“…it is surprising how incomplete our knowledge is today of the effects of direct current (DC) and low frequency voltages and currents on biological systems" [1]. Particular attention is paid to the response of protein to the increase in temperature, therefore important are techniques providing studies on thermal transitions [2][3][4][5]. Temperature dependence of DC electric conductivity (σ) is used in studies, which are carried out on polymeric materials, polymer-metal compounds, inorganic semiconductors [6,7] and variety of biological materials [8][9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

“…Studies on biological materials such as bones, hemoglobin, elastin, collagen by means of electric conductivity-temperature relationship (σ-T ) can provide useful information on phase transitions and water removal [3,10,11,[14][15][16][17]. Electric conductivity is sensitive to any change in chemical composition, structure of studied material and also permits to state whether the observed changes are permanent or temporary [18].…”

Section: Introductionmentioning

confidence: 99%

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DC Electrical Conductivity in Studies on Solid-State Proteins

Kubisz

Hojan‐Jezierska

2010

Acta Phys. Pol. A

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In structural studies on biological materials, among other methods, electrical techniques are used widely. Temperature dependence of electrical conductivity is a method permitting studies on denaturation, glass transition and water release -processes, which occur in solid-state proteins. Variations of amplitude and temperature of the peak on the recorded thermogram make it possible to draw conclusions about thermal stability and physicochemical processes occurring in the studied biological material. The shape of experimental curve is material-related and depends upon its "history". The paper is based on experimental results obtained mainly for collagen.

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Differential scanning calorimetry and temperature dependence of electric conductivity in studies on denaturation process of bone collagen

Cited by 23 publications

References 38 publications

Ultrafine heat-induced structural perturbations of bone mineral at the individual nanocrystal level

Ultrafine heat-induced structural perturbations of bone mineral at the individual nanocrystal level

Temperature dependence of electric conductivity of bamboo coral skeleton and glass sponge spicules, the marine origin biomaterials

DC Electrical Conductivity in Studies on Solid-State Proteins

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