Differential Sensing of MAP Kinases Using SOX‐Peptides

Zamora-Olivares, Diana; Kaoud, Tamer S.; Jose, Jiney; Ellington, Andrew D.; Dalby, Kevin N.; Anslyn, Eric V.

doi:10.1002/anie.201408256

Cited by 38 publications

(43 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[1][2][3][4][5][6] The development of "smart" noninvasive imaging reagents for the determination of specific enzyme activity in vivo is critically required for cancer diagnosis. [7][8][9][10][11][12][13][14][15] However, during the onset and progression of cancer, the dynamic living system complexity makes it difficult to track and visualize in vivo enzyme activity. As a typical enzyme, β-galactosidase (β-gal) has been demonstrated as an important biomarker for cell senescence and primary ovarian cancers.…”

Section: Introductionmentioning

confidence: 99%

Real-Time Tracking and In Vivo Visualization of β-Galactosidase Activity in Colorectal Tumor with a Ratiometric Near-Infrared Fluorescent Probe

et al. 2016

J. Am. Chem. Soc.

453

261

View full text Add to dashboard Cite

Development of "smart" noninvasive bioimaging probes for trapping specific enzyme activities is highly desirable for cancer therapy in vivo. Given that β-galactosidase (β-gal) is an important biomarker for cell senescence and primary ovarian cancers, we design an enzyme-activatable ratiometric near-infrared (NIR) probe (DCM-βgal) for the real-time fluorescent quantification and trapping of β-gal activity in vivo and in situ. DCM-βgal manifests significantly ratiometric and turn-on NIR fluorescent signals simultaneously in response to β-gal concentration, which makes it favorable for monitoring dynamic β-gal activity in vivo with self-calibration in fluorescent mode. We exemplify DCM-βgal for the ratiometric tracking of endogenously overexpressed β-gal distribution in living 293T cells via the lacZ gene transfection method and OVCAR-3 cells, and further realize real-time in vivo bioimaging of β-gal activity in colorectal tumor-bearing nude mice. Advantages of our system include light-up ratiometric NIR fluorescence with large Stokes shift, high photostability, and pH independency under the physiological range, allowing for the in vivo real-time evaluation of β-gal activity at the tumor site with high-resolution three-dimensional bioimaging for the first time. Our work provides a potential tool for in vivo real-time tracking enzyme activity in preclinical applications.

show abstract

Section: Introductionmentioning

confidence: 99%

Real-Time Tracking and In Vivo Visualization of β-Galactosidase Activity in Colorectal Tumor with a Ratiometric Near-Infrared Fluorescent Probe

et al. 2016

J. Am. Chem. Soc.

453

261

View full text Add to dashboard Cite

show abstract

“…Synthetic versions of such at ongue can be fairly primitive and just consist of ac ollection of dyes, for example. Anslyn et al, [4,5] Suslick et al, [6][7][8] Rotello et al [9][10][11] and other groups [12] have developed sensor fields that can be used to identify legionso fa nalytes. As ubset of such chemical tongues consistso fe lectrostatic complexes of af luorophore and aq uencher.A ne xample are the goldnanoparticle/PPE constructs by Rotello and ourselves.…”

mentioning

confidence: 99%

Polyelectrolyte Complexes Formed from Conjugated Polymers: Array‐Based Sensing of Organic Acids

Han

Bender

Hahn

et al. 2016

Chemistry A European J

View full text Add to dashboard Cite

One fluorescent, positively charged poly(para-phenyleneethynylene) (PAE 1) forms electrostatic complexes with five negatively charged pyridine- or benzothiadiazole-containing poly(para-aryleneethynylene)s (PAE 2-PAE 6). The PAE 2-PAE 6 are less fluorescent in water and act as quenchers for PAE 1 in their electrostatic complexes C 1-C 5; the PAE-complexes (2 μm) were exposed to thirteen different carboxylic acids (50 mm) in buffered aqueous solution. The fluorescence responses of the small library of electrostatic PAE-complexes towards the acids was analyzed; discrimination of all of the thirteen acids was achieved. The investigated acids include acetic, butyric, tartaric, maleic, lactic, sorbic, oxalic, aspartic, and citric acids. A random, simple, ad-hoc library of electrostatic polymer complexes, C 1-C 5, therefore discerns the thirteen carboxylic acids in water.

show abstract

“…Therefore, as in many other biochemical assays, the concentration of recognition elements, buffer, temperature, and measurement time should be constant during measurements. The use of regression analysis based on machine-learning techniques enables the quantitative identification of the compositions of analytes based on training data [ 53 ] and can therefore be applied to analytes of unknown concentration(s).…”

Section: Resultsmentioning

confidence: 99%

A Multichannel Pattern-Recognition-Based Protein Sensor with a Fluorophore-Conjugated Single-Stranded DNA Set

Okada

Sugai

Tomita

et al. 2020

Sensors

View full text Add to dashboard Cite

Recently, pattern-recognition-based protein sensing has received considerable attention because it offers unique opportunities that complement more conventional antibody-based detection methods. Here, we report a multichannel pattern-recognition-based sensor using a set of fluorophore-conjugated single-stranded DNAs (ssDNAs), which can detect various proteins. Three different fluorophore-conjugated ssDNAs were placed into a single microplate well together with a target protein, and the generated optical response pattern that corresponds to each environment-sensitive fluorophore was read via multiple detection channels. Multivariate analysis of the resulting optical response patterns allowed an accurate detection of eight different proteases, indicating that fluorescence signal acquisition from a single compartment containing a mixture of ssDNAs is an effective strategy for the characterization of the target proteins. Additionally, the sensor could identify proteins, which are potential targets for disease diagnosis, in a protease and inhibitor mixture of different composition ratios. As our sensor benefits from simple construction and measurement procedures, and uses accessible materials, it offers a rapid and simple platform for the detection of proteins.

show abstract

Differential Sensing of MAP Kinases Using SOX‐Peptides

Cited by 38 publications

References 47 publications

Real-Time Tracking and In Vivo Visualization of β-Galactosidase Activity in Colorectal Tumor with a Ratiometric Near-Infrared Fluorescent Probe

Real-Time Tracking and In Vivo Visualization of β-Galactosidase Activity in Colorectal Tumor with a Ratiometric Near-Infrared Fluorescent Probe

Polyelectrolyte Complexes Formed from Conjugated Polymers: Array‐Based Sensing of Organic Acids

A Multichannel Pattern-Recognition-Based Protein Sensor with a Fluorophore-Conjugated Single-Stranded DNA Set

Contact Info

Product

Resources

About