Differential sensitivities of viruses in red cell suspensions to methylene blue photosensitization

Abstract

MB-based protocols would require the use of high-efficiency (> 6log10) white cell-reduction filters or additional inactivation steps to deplete or inactivate intracellular virus.

Photochemical Treatment of Platelet Concentrates with a Novel Psoralen and UVA to Enhance the Safety of Platelet Transfusions_a

“…To date, this is the most extensive report documenting inactivation of a broad spectrum of infectious viruses and bacteria in full-sized platelet concentrates. Other potential decontamination systems have not demonstrated this degree of activity [28][29][30]. The PCT process described in this study is antiviral and antibacterial.…”

Effects of methylene blue‐treated plasma on red cells and stored platelet concentrates

“…Therefore, the simple addition of MBP to cellular components would not eliminate the risk of virus transmission. Additional inactivation steps would be required to deplete intracellular virus 28 . Studies of photo‐dynamic treatment of PCs have shown that direct exposure of platelets to MB photoinactivation cannot inactivate virus without damaging platelets 29 .…”

Removal of Methylene Blue from Plasma via an Adsorbent Filter

“…Although the technique also does not inactivate all nonenveloped viruses, the technique can be applied on each unit individually; thus the risk of transmission of these viruses is not increased by the MB process. However, by itself, MB treatment provides inadequate inactivation of intracellular viruses [10]. Thus, for the process to provide substantial assurance of inactivation, an additional step, such as disruption of leukocytes via a freeze/thaw cycle, is necessary.…”