Differential sensitivity of Allium cepa L. and Vicia faba L. to aqueous extracts of Cascabela thevetia (L.) Lippold

Basu, Surochita; Tripura, Kanika

doi:10.1016/j.sajb.2021.01.033

Cited by 10 publications

(5 citation statements)

References 33 publications

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“…At least 1000 cells on each slide and five slides for each concentration were examined. the results obtained on root growth and structure are in agreement with the results of Basu and Tripura (2021) on Cascabela thevetia who found a decrease in root growth, turgescence, and color change in A. cepa and V. faba treated with a high concentration of Cascabela thevetia extract. Similar results were approved by the studies of Issa et al (2020) on the roots of Avena fatua and Echinochloa crus-galli exposed to a high concentration of Vitex negundo.…”

Section: Discussionsupporting

confidence: 90%

Cytogenetic effects of Tribulus terrestris L. on meristematic cells of Allium cepa L. and Vicia faba L.

Bouzekri,

Nassar,

Slimani

et al. 2023

Caryologia

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Tribulus terrestris is a plant of the Zygophyllaceae family frequently used worldwide to treat various diseases due to the therapeutic effects of its pharmacological components. This study examines the cytotoxic and genotoxic effects of T. terrestris using two plant models, Allium cepa and Vicia faba. Extracts of 0.00625, 0.0125, 0.025, 0.05 and 0.1mg/mL were tested on meristematic cells of A. cepa and V. faba roots. This assessment includes the study of root growth, structure and coloration, as well as the determination of the mitotic index (MI) and chromosomal aberrations (CAs) as accurate indicators of toxicity. Our results showed a significant decrease in the mean length of roots treated with 0.025, 0.05 and 0.1 mg/ml for A. cepa and 0.1 mg/ml for V. faba. Cytotoxicity and genotoxicity results showed a significant decrease in MI from 0.025 mg/ml in A. cepa and from 0.05 mg/ml in V. faba, and this decrease in MI is linked to the increase in concentration and treatment time with T. terrestris. Furthermore, a significant increase in CAs was observed in A. cepa and V. faba from the 0.025 mg/ml concentration. The significant reduction in MI and CAs abundance suggests the genotoxicity of T. terrestris. Therefore, T. terrestris is a medicinal plant that should be used with caution, appropriately and based on essential therapeutic needs.

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Section: Discussionsupporting

confidence: 90%

Cytogenetic effects of Tribulus terrestris L. on meristematic cells of Allium cepa L. and Vicia faba L.

Bouzekri,

Nassar,

Slimani

et al. 2023

Caryologia

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“…At least eight root tips were observed in each treatment group, and an average of 1000 cells were scored per root tip. Mitotic index (MI%), micronucleus frequency (MN‰), chromosomal aberration frequency (CA%) were calculated from the following formula (Basu and Tripura, 2021; Ma et al, 2012):…”

Section: Methodsmentioning

confidence: 99%

“…The root meristem of Vicia faba and Allium cepa is a good test system for genotoxicity analysis (Srivastava and Mishra, 2009; Akbaba, 2020). At present, the Vicia and Allium root micronucleus (MN) tests have been widely used in soil contaminantion (Gupta et al, 2022), radiation damage (Kuma et al, 2020), antibiotics (Khadra et al, 2019), dyes (Alaguprathana et al, 2022), and even plant extracts (Basu and Tripura, 2021). Vicia faba has a large chromosome, that is easy to identify mitotic phases and chromosomal abnormality and has high sensitivity, easy operation, and strong reliability (Dhyèvre et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Cytotoxicity and genotoxicity evaluation of chloroform using Vicia faba roots

Zhang

et al. 2023

Toxicol Ind Health

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Chloroform is a widely used industrial chemical that can also pollute the environment. The aims of this study were to examine the potential cytotoxicity and genotoxicity of chloroform on plant cells, using the Vicia faba bioassay. Chloroform was evaluated at concentrations of 0.1, 0.5, 1, 2, and 5 mg·L−1. The following parameters were analyzed: the mitotic index (MI), micronucleus (MN) frequency, chromosomal aberration (CA) frequency, and malondialdehyde (MDA) content. The results showed that exposure to increasing concentrations of chloroform caused a decrease in MI and an increase in the frequency of MN in Vicia faba root tip cells, relative to their controls. Moreover, various types of CA, including C-mitosis, fragments, bridges, laggard chromosomes, and multipolar mitosis, were observed in the treated cells. The frequency of MN was positively correlated with the frequency of CA in exposure to 0.1–1 mg·L−1 chloroform. Furthermore, chloroform exposure induced membrane lipid peroxidation damage in the Vicia faba radicle, and a linear correlation was observed between the MDA content and the frequency of MN or CA. These findings indicated that chloroform exposure can result in oxidative stress, cytotoxicity, and genotoxicity in plant cells.

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“…In general, plants belonging to the genus Allium, especially Allium cepa , are considered the most useful and effective for genotoxicity assessment due to their advantages [ 42 , 50 ]. Among the many advantages are low number of chromosomes ( Allium cepa 2n = 16), large chromosomes, large genomes (>15,000 Mbp) [ 51 ], roots that can grow in direct contact with any tested substance, easy identification of mitotic phases and chromosomal aberrations [ 52 ], rare occurrence of spontaneous chromosomal lesions, fast response to genotoxic compounds, diversity in chromosome morphology, low cost, and easy to handle [ 50 , 53 , 54 ].…”

Section: Introductionmentioning

confidence: 99%

Effects of Indigo Carmine on Growth, Cell Division, and Morphology of Allium cepa L. Root Tip

Ristea,

Zarnescu

2024

Toxics

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Indigo carmine has a variety of uses in foods, textiles, medicine, pharmaceuticals, and cosmetics. There are studies reporting the toxic potential of indigo carmine on human health and the environment. In this study, we investigated the cytogenotoxic effects of indigo carmine using apical root cells of Allium cepa. Allium cepa bulbs were subjected to four treatments with indigo carmine (0.0032, 0.0064, 0.0125, and 0.2 mg/mL) and to ultrapure water as a control. After 5 days, root growth, root length, mitotic index, mitotic inhibition, chromosomal anomalies, and cell morphology were analyzed. According to our results, a decrease in root length and mitotic index was observed at all concentrations of indigo carmine. Additionally, several types of chromosomal abnormalities were observed, such as disturbed metaphase, sticky chain metaphase, anaphase bridge, and laggard chromosomes. Moreover, histological observation indicated that indigo carmine induces alterations in various components of root tip tissue, such as deformation and alteration of the cell wall, progressive condensation of chromatin, shrinkage of the nuclei, and an increase in the number of irregularly shaped nuclei and nuclear fragments. Our results indicate that the tested concentrations of indigo carmine may have toxic effects and raise concerns about its intensive use in many fields.

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Differential sensitivity of Allium cepa L. and Vicia faba L. to aqueous extracts of Cascabela thevetia (L.) Lippold

Cited by 10 publications

References 33 publications

Cytogenetic effects of Tribulus terrestris L. on meristematic cells of Allium cepa L. and Vicia faba L.

Cytogenetic effects of Tribulus terrestris L. on meristematic cells of Allium cepa L. and Vicia faba L.

Cytotoxicity and genotoxicity evaluation of chloroform using Vicia faba roots

Effects of Indigo Carmine on Growth, Cell Division, and Morphology of Allium cepa L. Root Tip

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