Differential sensitivity of Chironomus and human hemoglobin to gamma radiation

Gaikwad, Pallavi S.; Panicker, Lata; Mohole, Madhura; Sawant, Sangeeta; Mukhopadhyaya, Robin; Nath, Bimalendu B.

doi:10.1016/j.bbrc.2016.05.129

Cited by 5 publications

(4 citation statements)

References 24 publications

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“…On the other hand, Hu-Hb showed molecular damage and a shift in Soret band when subjected to similar dose of gamma radiation. Unlike Ch-Hb, additional biophysical analyses indicated relatively higher sensitivity of Hu-hb with reference to heme pocket of Hb molecules (Gaikwad et al, 2016). This was interesting to note from the evolutionary point of view because Hu-Hb represents vertebrate Hbs which underwent little changes since the time of divergence of vertebrates and invertebrates.…”

Section: Radiationmentioning

confidence: 90%

“…Pune University, Pune, India) who established chironomid midges as one of the insect groups tolerant to high doses of gamma radiation (Datkhile et al, 2009a(Datkhile et al, , 2009b(Datkhile et al, , 2011(Datkhile et al, , 2015. Recently the focus of this research group shifted to Hb of C. ramosus larvae subjected to high doses of gamma radiation and a few interesting facts got revealed (Kumar et al, 2017, Gaikwad et al, 2016 which are worth mentioning in this review.…”

Section: Radiationmentioning

confidence: 94%

“…This was interesting to note from the evolutionary point of view because Hu-Hb represents vertebrate Hbs which underwent little changes since the time of divergence of vertebrates and invertebrates. A sequence and structure-based bioinformatic analysis (Gaikwad et al, 2016 and unpublished data of Sawant and Nath) revealed higher hydrophobicity around heme pockets of Ch-Hb when compared to Hu-Hb. The remarkable resistivity of chironomid midges to adverse environmental conditions in general could be attributed to hydrophobicity of heme pockets of Ch-Hb and molecular evolutionary history of this group of insects.…”

Section: Radiationmentioning

confidence: 94%

“…In the literature, three-dimensional structure of deoxy hemoglobin (component III) from Chironomus thummi thummi has remained the only source for a reference structure to carry out biochemical and bioinformatics studies (Gaikwad et al 2016). From the evolutionary point of view, it is important to note that amino acid in heme binding pockets of Hb molecules underwent drastic evolutionary diversifications resulting in complete absence of amino acids like isoleucine and glutamine in human Hb during the course of evolution since the speciation of non-biting chironomid midges (Tab.…”

Section: Biochemical Featuresmentioning

confidence: 99%

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Extracellular hemoglobin and environmental stress tolerance in Chironomus larvae

Nath

2018

J Limnol

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Hemoglobin (Hb) is one of the most common conserved molecules found in organisms belonging to all major kingdoms of life. Chironomid midge larvae are unique among the invertebrates being the only free-living group of organisms possessing extracellular hemoglobins (Hbs) in monomeric and dimeric forms floating in their hemolymph. Remarkable ability of individual species of chironomid midges to tolerate wide range of environmental stressors prevailing in their respective ecosystems has enabled chironomids to sustain as one of the most widely distributed insects in the world. Ability of different Chironomus spp to thrive under extreme hypoxic conditions as well as in the presence of chemical pollution made chironomid midges an efficient model system to assess the effect of environmental stress in different freshwater ecosystems. The modulation of Hb concentration has been found to be a function of different type of abiotic stressors and thus Hb of chironomid midges (Ch-Hb) has attracted the attention as a potential biomarker for environmental biomonitoring. Large body of literature on Ch-Hb accumulated mainly during sixties to eighties of twentieth century has enriched our understanding of its physiological, developmental and biochemical features. Empirical as well as in-silico studies carried out in recent years provided insights into many structure-function relationships of Ch-Hb. However, for the last few decades, majority of the studies were carried out to project and validate Ch-Hb as potential bioindicator for evaluating toxicants, chemical pollutants and environmental gradients of dissolved oxygen concentration. In this review, historical development of the subject has been compiled with notes on future implications of contemporary studies on Ch-Hb.

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Section: Radiationmentioning

confidence: 90%