Differential staining of acid glycosaminoglycans (mucopolysaccharides) by Alcian blue in salt solutions

Scott, J. E.; Dorling, J.

doi:10.1007/bf00306130

Cited by 984 publications

(352 citation statements)

References 15 publications

Supporting

Mentioning

339

Contrasting

Unclassified

Order By: Relevance

“…Normal and hyperplastic mammary glands, as well as benign and malignant tumours of different types, were studied light microscopically. Tissues were stained with alcian blue combined with the 'critical electrolyte concentration' (CEC) (Scott and Dorling, 1965) and enzyme or nitrous acid degradation of specific GAGs. In addition, immunohistochemistry was performed, to detect CS in the tissue.…”

mentioning

confidence: 99%

Stromal accumulation of chondroitin sulphate in mammary tumours of dogs

1999

View full text Add to dashboard Cite

To contribute to the investigation of the composition of the extracellular matrix in epithelial tumours, mammary gland tissues of dogs (including tumours, hyperplasias and normal tissue as well as metastatic lesions in lymph nodes and lung) were studied histochemically and immunohistochemically for distribution of sulphated glycosaminoglycans (s-GAGs). The formaline-fixed tissue was stained by alcian blue at pH 5.8, using the ‘critical electrolyte concentration’ to study the degree of sulphation of s-GAGs. s-GAGs were characterized by degradation with enzymes and nitrous acid and by immunohistochemistry with two anti-chondroitin sulphate monoclonal antibodies. The light microscopic investigation of s-GAG deposits revealed a limited number of patterns of their distribution. The main s-GAGs found in the mammary gland tumours of dogs and in metastatic lesions were chondroitin sulphate (CS) and heparin/heparan sulphate (HEP/HS). CS accumulated in diffuse structures between epithelial cells as well as around clusters of tumour cells. The latter pattern, possibly representing a mesenchymal reaction to the tumour, was present in 74% of the tumours, and in 67% of these, highly sulphated CS was present. A diffuse accumulation of CS was present almost exclusively in complex and mixed tumours; because of the expression of the 3B3 epitope for CS in immature cartilage the spindle cells of complex tumours are argued to be the precursors of the cartilage in mixed tumours. HEP/HS was stored mainly in mast cells that were found in increased numbers in hyperplasias and tumours. By pretreatment of microscopic slides with chondroitinase AC or ABC immunostaining of fibronectin could be made possible in areas in which CS was abundantly present, suggesting that CS may mask fibronectin epitopes. It is concluded that CS with different degrees of sulphation is the most important s-GAG in the extracellular matrix of mammary tumours of dogs. CS and other s-GAGs accumulate at different sites and may have a different pathogenetic significance. © 1999 Cancer Research Campaign

show abstract

mentioning

confidence: 99%

Stromal accumulation of chondroitin sulphate in mammary tumours of dogs

1999

View full text Add to dashboard Cite

show abstract

“…3) also secrete highly sulphated adhesives with small carboxyl components. The results with Grammatophora differ from those of other species since only the centres of the adhesive links stain with the PAS reaction, while the protein specific Chayen et al (1973) McCully (1966) Chayen et al (1973) Pearse (1968) Blanquet (1976) Parker & Diboll (1966 Scott & Dorling (1965) Pearse (1968) Heath (1961) Hollander (1964) McGee-Russell (1955 ninhydrin-Schiff procedure produces the reverse effect. This is almost certainly due in part to a coating of bacteria and claymineral particulates, but the positive localization of acidic groups in the adhesive may indicate a predominance of these in the outer regions of the pad.…”

Section: Pad-forming Speciesmentioning

confidence: 89%

Cytochemical and electron microscopical observations on the adhesive materials of marine fouling diatoms

Daniel

Chamberlain

Jones

1987

British Phycological Journal

View full text Add to dashboard Cite

“…Histochemistry-Sections were stained with Alcian blue 8GX using described methods (26,27). Immunolabeling of longitudinal control and immobilized limb sections for polymerized actin, uridine diphosphoglucose dehydrogenase (UDPGD), CD44, and moesin used, respectively, (i) 1 g ml Ϫ1 fluorescein isothiocyanate-conjugated phalloidin (Sigma), (ii) 1:10 purified rabbit antibody raised against a bovine UDPGD C-terminal synthetic peptide ( 458 IETIGKKVSS 467 ; data base A54926S; a gift from Roger Mason and Mark Chambers) followed by 1:50 biotinylated goat anti-rabbit IgG in Tris-buffered saline/Tween with 20% filtered chick serum (1 h), detected using avidin-conjugated glucose oxidase complex (ABC-GO kit; Vector Laboratories) and substrate (GO-NBT substrate kit; Vector) according to the manufacturer's instructions, (iii) 1:100 mouse anti-chick CD44 (a gift from F. Davison and C. Tregaskes) visualized using glucose oxidase complex (ABC-GO kit; Vector) as described herein, and (iv) anti-hamster kidney cell moesin visualized using the appropriate streptavidin-conjugated alkaline phosphatase secondary antibody (4) and reacted in medium containing naphthol-AS-MX phosphate, levamisole, and Fast Blue BB salt.…”

Section: Methodsmentioning

confidence: 99%

Selective Activation of the MEK-ERK Pathway Is Regulated by Mechanical Stimuli in Forming Joints and Promotes Pericellular Matrix Formation

Bastow

Lamb

Lewthwaite

et al. 2005

Journal of Biological Chemistry

View full text Add to dashboard Cite

It is well established that local modification of extracellular matrix (ECM) hyaluronan composition is vital in the regulation of cell behavior. Indeed, the formation of articulating chick joint cavities, which requires mechanical stimuli derived from skeletal movement, is dependent upon the accumulation of an ECM rich in hyaluronan (HA). However, the mechanisms responsible for such precise mechano-dependent regulation of cell behavior and the formation of a HA-rich ECM remain undefined. Here we show that extracellular-regulated kinase 1/2 (ERK1/2) is selectively activated in cells at sites of cavity formation and activity diminished by in ovo immobilization that induces cartilaginous fusion across presumptive joint interzones. In vitro analyses offer mechanistic support for the role of mechanical stimuli in promoting a MEK-dependent activation of ERK1/2. In addition, our direct regulation of ERK1/2 phosphorylation status via modulation of its up-stream "classical cascade" activator either pharmacologically or by transfection with dominant negative or constitutively active Mek confirms the essential role for ERK1/2 activation in the elaboration of HA-rich pericellular matrices. Together, our findings demonstrate that the MEK-ERK pathway, regulated by mechanical stimuli, controls HA-rich matrix assembly. The precision of ERK1/2 activation selectively distinguishing cells at the joint line suggests that it directly contributes to the loss of tissue cohesion essential for generating HA-rich cavities between joint elements during their development.

show abstract

Differential staining of acid glycosaminoglycans (mucopolysaccharides) by Alcian blue in salt solutions

Cited by 984 publications

References 15 publications

Stromal accumulation of chondroitin sulphate in mammary tumours of dogs

Stromal accumulation of chondroitin sulphate in mammary tumours of dogs

Cytochemical and electron microscopical observations on the adhesive materials of marine fouling diatoms

Selective Activation of the MEK-ERK Pathway Is Regulated by Mechanical Stimuli in Forming Joints and Promotes Pericellular Matrix Formation

Contact Info

Product

Resources

About