Differential stress gene expression during the development of Neurospora crassa and other fungi

Rensing, Ludger

doi:10.1016/s0378-1097(98)00416-9

Cited by 19 publications

(29 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Moreover, microscopic analysis revealed that afp::uidA transcription is temporally and spatially regulated during development. This is consistent with the observation that heat shock proteins in Neurospora crassa are dierentially expressed during development and that the interactions between the heat shock transcription factor and HSE dier signi®cantly depending on developmental stage (Rensing et al 1998;Girvitz et al 2000). Developmental cycles in fungi are often controlled by stress-inducing signals.…”

Section: Discussionsupporting

confidence: 86%

Transcriptional regulation of the Antifungal Protein in Aspergillus giganteus

2002

View full text Add to dashboard Cite

Regulation of the expression of the afp gene that codes for the Antifungal Protein of Aspergillus giganteus was investigated using a reporter system. For this purpose, the E. coli reporter gene uidA encoding beta-glucuronidase (GUS) was placed under the control of the afp promoter. No homologous integration of the reporter construct into the afp site was observed among 156 transformants tested. In one of the transformants carrying a single, ectopically integrated, copy of the construct, GUS and AFP both displayed exactly the same temporal expression patterns under various cultivation conditions, as assayed by Northern and protein analyses. Thus, this transformant was used to identify factors that are involved in the transcriptional regulation of afp expression. Expression is only detectable in the vegetative mycelium, whereas no expression occurs in aerial hyphae or conidia, indicating that afp expression is developmentally regulated. Transcription of afp is regulated by ambient pH, being suppressed under acidic conditions and strongly induced under alkaline conditions. This observation suggests that PacC regulates the afp gene, which is consistent with the presence of two putative PacC binding sites within the 5' upstream region. Transcription is not subject to carbon catabolite repression or nitrogen metabolite repression. The expression of afp is up-regulated by heat shock, upon growth in the presence of excess NaCl and ethanol, and under conditions of carbon starvation. In contrast, expression decreases slightly in the presence of hydrogen peroxide and under nitrogen starvation. These data are compatible with the presence of a putative heat shock element (NTTCNNGANTTCN) and five putative C(4)T stress-responsive elements within the afp promoter.

show abstract

Section: Discussionsupporting

confidence: 86%

Transcriptional regulation of the Antifungal Protein in Aspergillus giganteus

2002

View full text Add to dashboard Cite

show abstract

“…Differences in these two forms of dormancy have been studied primarily in model organisms [49,54], but if constitutive dormancy is a common feature across a broad range of fungal taxa [53] and is randomly distributed, it could influence our ability to infer patterns of active community assembly in fungi across environmental gradients.…”

Section: The Role Of Dormancy In Bacterial and Eukaryotic Communitiesmentioning

confidence: 99%

Assembly of Active Bacterial and Fungal Communities Along a Natural Environmental Gradient

Mueller

Gallegos-Graves

Zak³

et al. 2015

Microb Ecol

View full text Add to dashboard Cite

Dormancy is thought to promote biodiversity within microbial communities, but how assembly of the active community responds to changes in environmental conditions is unclear. To measure the active and dormant communities of bacteria and fungi colonizing decomposing litter in maple forests, we targeted ribosomal genes and transcripts across a natural environmental gradient. Within bacterial and fungal communities, the active and dormant communities were phylogenetically distinct, but patterns of phylogenetic clustering varied. For bacteria, active communities were significantly more clustered than dormant communities, while the reverse was found for fungi. The proportion of operational taxonomic units (OTUs) classified as active and the degree of phylogenetic clustering of the active bacterial communities declined with increasing pH and decreasing C/N. No significant correlations were found for the fungal community. The opposing pattern of phylogenetic clustering in dormant and active communities and the differential response of active communities to environmental gradients suggest that dormancy differentially structures bacterial and fungal communities.

show abstract

“…Transcription of heat shock genes has been well documented in N. crassa (Britton and Kapoor 2002;Freitag et al 1997;Kapoor et al 1995;Plesofsky et al 2008;Rensing et al 1998;Tremmel et al 2007;Ungermann et al 1994) as well as in other filamentous fungi (Faircloth et al 2009;Fox et al 2007;Georg and Gomes 2007;MonteroBarrientos et al 2008;Rezaie et al 2000;Turkel et al 2006;Xavier et al 1999). However, little is known about the cell stress response to phosphate (Pi) deprivation and changes in extracellular pH.…”

Section: Introductionmentioning

confidence: 99%

Transcription of the Neurospora crassa 70-kDa class heat shock protein genes is modulated in response to extracellular pH changes

Squina

Leal

Cipriano

et al. 2010

Cell Stress and Chaperones

View full text Add to dashboard Cite

Heat shock proteins belong to a conserved superfamily of molecular chaperones found in prokaryotes and eukaryotes. These proteins are linked to a myriad of physiological functions. In this study, we show that the N. crassa hsp70-1 (NCU09602.3) and hsp70-2 (NCU08693.3) genes are preferentially expressed in an acidic milieu after 15 h of cell growth in sufficient phosphate at 30°C. No significant accumulation of these transcripts was detected at alkaline pH values. Both genes accumulated to a high level in mycelia that were incubated for 1 h at 45°C, regardless of the phosphate concentration and extracellular pH changes. Transcription of the hsp70-1 and hsp70-2 genes was dependent on the pacC + background in mycelia cultured under optimal growth conditions or at 45°C. The pacC gene encodes a Zn-finger transcription factor that is involved in the regulation of gene expression by pH. Heat shock induction of these two hsp genes in mycelia incubated in low-phosphate medium was almost not altered in the nuc-1 − background under both acidic and alkaline pH conditions. The NUC-1 transcriptional regulator is involved in the derepression of nucleases, phosphatases, and transporters that are necessary for fulfilling the cell's phosphate requirements. Transcription of the hsp70-3 (NCU01499.3) gene followed a different pattern of induction-the gene was depressed under insufficient phosphate conditions but was apparently unaffected by alkalinization of the culture medium. Moreover, this gene was not induced by heat shock. These results reveal novel aspects of the heatsensing network of N. crassa.

show abstract

Differential stress gene expression during the development of Neurospora crassa and other fungi

Cited by 19 publications

References 40 publications

Transcriptional regulation of the Antifungal Protein in Aspergillus giganteus

Transcriptional regulation of the Antifungal Protein in Aspergillus giganteus

Assembly of Active Bacterial and Fungal Communities Along a Natural Environmental Gradient

Transcription of the Neurospora crassa 70-kDa class heat shock protein genes is modulated in response to extracellular pH changes

Contact Info

Product

Resources

About