Differential stress resistance and metabolic traits underlie coexistence in a sympatrically evolved bacterial population

Puentes-Téllez, Pilar Eliana; Elsas, Jan Dirk van

doi:10.1111/1462-2920.12551

Cited by 4 publications

(3 citation statements)

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“…The Cre-loxP or FLP-FRT marker rescue system can be easily used in natural S. cerevisiae and E. coli strains avoiding screening of auxotrophs [ 38 , 39 ]. Recent studies on natural populations and experimental evolution of E. coli identified strains exhibiting special properties, including tolerance to environmental stresses, utilization of particular carbon sources such as galactose, and low toxicity [ 40 , 41 ]. Based on this study and the background knowledge, we suggested that specific strains of model microorganisms can be used as bioengineering hosts for special applications.…”

Section: Discussionmentioning

confidence: 99%

Engineering a natural Saccharomyces cerevisiae strain for ethanol production from inulin by consolidated bioprocessing

Wang

2016

Biotechnol Biofuels

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BackgroundThe yeast Saccharomyces cerevisiae is an important eukaryotic workhorse in traditional and modern biotechnology. At present, only a few S. cerevisiae strains have been extensively used as engineering hosts. Recently, an astonishing genotypic and phenotypic diversity of S. cerevisiae was disclosed in natural populations. We suppose that some natural strains can be recruited as superior host candidates in bioengineering. This study engineered a natural S. cerevisiae strain with advantages in inulin utilization to produce ethanol from inulin resources by consolidated bioprocess. Rational engineering strategies were employed, including secretive co-expression of heterologous exo- and endo-inulinases, repression of a protease, and switch between haploid and diploid strains.ResultsResults from co-expressing endo- and exo-inulinase genes showed that the extracellular inulinase activity increased 20 to 30-fold in engineered S. cerevisiae strains. Repression of the protease PEP4 influenced cell physiology in late stationary phase. Comparison between haploid and diploid engineered strains indicated that diploid strains were superior to haploid strains in ethanol production albeit not in production and secretion of inulinases. Ethanol fermentation from both inulin and Jerusalem artichoke tuber powder was dramatically improved in most engineered strains. Ethanol yield achieved in the ultimate diploid strain JZD-InuMKCP was close to the theoretical maximum. Productivity achieved in the strain JZD-InuMKCP reached to 2.44 and 3.13 g/L/h in fermentation from 200 g/L inulin and 250 g/L raw Jerusalem artichoke tuber powder, respectively. To our knowledge, these are the highest productivities reported up to now in ethanol fermentation from inulin resources.ConclusionsAlthough model S. cerevisiae strains are preferentially used as hosts in bioengineering, some natural strains do have specific excellent properties. This study successfully engineered a natural S. cerevisiae strain for efficient ethanol production from inulin resources by consolidated bioprocess, which indicated the feasibility of natural strains used as bioengineering hosts. This study also presented different properties in enzyme secretion and ethanol fermentation between haploid and diploid engineering strains. These findings provided guidelines for host selection in bioengineering.Electronic supplementary materialThe online version of this article (doi:10.1186/s13068-016-0511-4) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Engineering a natural Saccharomyces cerevisiae strain for ethanol production from inulin by consolidated bioprocessing

Wang

2016

Biotechnol Biofuels

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“…Despite the possible potential of natural strain as a bioengineering host, detailed background studies must first be carried out so as to avoid futile adventure. This is because genetic manipulation may not be as easy as observed in model strains (Puentes‐Tellez & van Elsas, 2015; Wang, Li, & Wang, 2016).…”

Section: Challenges Of Yeast Strains Developmentmentioning

confidence: 99%

“…be as easy as observed in model strains(Puentes-Tellez & van Elsas, 2015;Wang, Li, & Wang, 2016).Having overcome the choice of feedstock and yeast strain to engineer for bioethanol production, employing the right strategy for strain development is key. Various engineering techniques have been used for yeast strain development either for improved substrate utilization, increased stress, toxic and thermo-tolerance abilities, or improved bioethanol production.…”

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Genetics and metabolic engineering of yeast strains for efficient ethanol production

Adebami

Kuila

Ajunwa

et al. 2021

J Food Process Engineering

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Bioethanol production from monomeric sugar is performed by several yeasts. But there are several limitations associated with yeast strains such as their low tolerance to ethanol, toxic inhibitors, and high sugar concentration. Genetic and metabolic engineering of potential yeast strains can overcome the above limitations. The present article summarized current genetic and metabolic engineering approaches for the development of yeast strain for efficient ethanol production. The review systematically examined bioethanol generations based on substrate utilization, criteria for strain selections, strategies for strain improvements including randomized mutagenesis, genetic engineering, metabolic engineering, genome editing, whole genome (re)sequencing, promoter engineering, quantitative trait locus analysis, protein engineering, and evolutionary engineering. Different fermentation technologies employed in hydrolysate fermentation including low gravity (LG), high gravity (HG), and very high gravity (VHG) as well as challenges of yeast strains development and its future prospect have been critically evaluated in this article. Significant engineering efforts are imminent for yeast‐based second‐generation biofuel to leave a demonstration phase through strain improvement and become economically competitive with fossil fuel. Practical Applications This is a comprehensive review of yeast strain development for bioethanol production. The readers should be able to acquire some basic knowledge on: The accompanied substrates for bioethanol generations as well as the technologies and challenges behind them. The criteria to consider in selecting yeast strain for bioengineering development. Different strategies and their reported applications employed in yeast strain development including randomized mutagenesis, genetic engineering, metabolic engineering, genome editing, whole genome (re)sequencing, promoter engineering, quantitative trait locus (QTL) analysis, protein engineering, and evolutionary engineering. Challenges and merits of different fermentation technologies employed in hydrolysate fermentation including LG, HG, and VHG. Possible challenges to encounter in developing yeast strain for bioethanol production. Desirable traits to consider in the selection and development of yeast strains for bioethanol production.

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Transmission in the Origins of Bacterial Diversity, From Ecotypes to Phyla

Cohan

2019

Microbial Transmission

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Any two lineages, no matter how distant they are now, began their divergence as one population splitting into two lineages that could coexist indefinitely. The rate of origin of higher-level taxa is therefore the product of the rate of speciation times the probability that two new species coexist long enough to reach a particular level of divergence. Here I have explored these two parameters of disparification in bacteria. Owing to low recombination rates, sexual isolation is not a necessary milestone of bacterial speciation. Rather, irreversible and indefinite divergence begins with ecological diversification, that is, transmission of a bacterial lineage to a new ecological niche, possibly to a new microhabitat but at least to new resources. Several algorithms use sequence data from a taxon of focus to identify phylogenetic groups likely to bear the dynamic properties of species. Identifying these newly divergent lineages allows us to characterize the genetic bases of speciation, as well as the ecological dimensions upon which new species diverge. Speciation appears to be least frequent when a given lineage has few new resources it can adopt, as exemplified by photoautotrophs, C1 heterotrophs, and obligately intracellular pathogens; speciation is likely most rapid for generalist heterotrophs. The genetic basis of ecological divergence may determine whether ecological divergence is irreversible and whether lineages will diverge indefinitely into the future. Long-term coexistence is most likely when newly divergent lineages utilize at least some resources not shared with the other and when the resources themselves will coexist into the remote future.

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Differential stress resistance and metabolic traits underlie coexistence in a sympatrically evolved bacterial population

Cited by 4 publications

References 55 publications

Engineering a natural Saccharomyces cerevisiae strain for ethanol production from inulin by consolidated bioprocessing

Engineering a natural Saccharomyces cerevisiae strain for ethanol production from inulin by consolidated bioprocessing

Genetics and metabolic engineering of yeast strains for efficient ethanol production

Transmission in the Origins of Bacterial Diversity, From Ecotypes to Phyla

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