Differential Subunit Aggregation of a Purified Protein from Cold-hardened and Unhardened Puma Rye

Hüner, N. P. A.; Carter, J.V.

doi:10.1016/s0044-328x(82)80081-0

Cited by 9 publications

(2 citation statements)

References 12 publications

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“…Under frost conditions, aggregation, especially of the LSU, appears to be a major reason for inactivation (Huner and Carter 1982), and we also observed frost-induced protein aggregation in vitro. Since two-dimensional electrophoresis showed no differences between the enzyme from control and chilled plants, significant amounts of defined protein digestion seem improbable.…”

Section: Discussionsupporting

confidence: 68%

Long-term chilling of young tomato plants under low light

Brggemann¹,

Klaucke²,

Maas-Kantel³

1994

Planta

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The properties of two Calvin-cycle key enzymes, i.e. stromal fructose-1,6-bisphosphatase (sFBPase) and ribulose-l,5-bisphosphate carboxylase/oxygenase (Rubisco) were studied in the cultivated tomato (Lycopersicon esculentum Mill.) and in four lines of a wild tomato (L. peruvianum Mill.) from different altitudes. During chilling for 14 d at 10~ and low light, the activation energy (EA) of the reaction catalyzed by sFBPase decreased by 5-10 kJ.mol 1 in L. esculentum and the three L. peruvianum lines from high altitudes. In L. peruvianum, no loss or only small losses of enzyme activity were observed during the chilling. Together with the change in EA, this indicates that the latter species is able to acclimate its Calvin-cycle enzymes to low temperatures. In L. esculentum, the chilling stress resulted in the irreversible loss of 57% of the initial sFBPase activity. Under moderately photoinhibiting chilling conditions for 3 d, the L. peruvianum line from an intermediate altitude showed the largest decreases in both the ratio of variable to maximum chlorophyll fluorescence (Fv/Fm) and the in-vivo activation state of sFBPase, while the other L. peruvianum lines showed no inhibition of sFBPase activation. Ribulose-l,5-bisphosphate carboxylase/oxygenase was isolated by differential ammonium-sulfate precipitation and gel filtration and characterized by two-dimensional electrophoresis. The enzyme from L. esculentum had three isoforms of the small subunit of Rubisco, each with different isoelectric points. Of these, the L. peruvianum enzyme contained only the two more-acidic isoforms. Arrhenius plots of the specific activity of purified Rubisco showed breakpoints at approx. 17~ Upon chilling, the Abbreviations: a.s.l. = above sea level; Chl = chlorophyll; DTT = dithiothreitol; E A = activation energy; FBP = fructose-1,6-bisphosphate; Fv/F m = ratio of variable to maximum chlorophyll fuorescence; HL = high light (500 gmol photons.m 2.s 1); LSU = large subunit of Rubisco; ME = 2-mercaptoethanol; Rubisco = ribulose-l,5-bisphosphate carboxylase/oxygenase; RuBP = ribulose-l,5-bisphosphate; sFBPase = stromal fructose-1,6-bisphosphatase; SSU = small subunit of Rubisco Correspondence to: W. Brfiggemann; FAX: 49 (211) 311 3085 specific activity of the enzyme from L. esculentum decreased by 5 1%, while E A below the breakpoint temperature increased from 129 to 189 kJ-mol 1. In contrast, Rubisco from the L. peruvianum lines from high altitudes was unaffected by chilling. We tested several possibile explanations for Rubisco inactivation, using two-dimensional electrophoresis, analytical ultracentrifugation, gel filtration and inhibitor tests. No indications were found for differential expression of the subunit isoforms, proteolysis, aggregation, subunit disassembly, or inhibitor accumulation in the enzyme from chilled L. esculentum. We suggest that the activity loss in the L. esculentum enzyme upon chilling is the result of a modification of sulfhydryl groups or other sidechains of the protein.

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Section: Discussionsupporting

confidence: 68%

Long-term chilling of young tomato plants under low light

Brggemann¹,

Klaucke²,

Maas-Kantel³

1994

Planta

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“…The PgRab7 gene was upregulated by dehydration in Pennisetum glaucum [44], while overexpression of the peanut AhRabG3f exhibited an enhanced tolerance to drought stress in transgenic peanut (Arachis hypogaea L.) [45] but negative role in Arabidopsis. Calciumbinding proteins (CaBs), such as calmodulin (CaM), calcium-sensing receptor (CaSR), calreticulin (CRT), and calcium-dependent protein kinase (CDPK), enhanced the survival of Triticum aestivum [46], and several protein kinases (e.g., serine/threonine-protein kinase, germinal center kinase (GCK)-like kinase Plant Defense Mechanisms MIK, receptor-like protein kinase HERK 1-like, phototropin family protein kinase, and salt-inducible protein kinase), imply their role in drought response signaling pathway, in addition to phosphorylation level of protein phosphate 2C (PP2C), acts as negative regulator for plant drought tolerance in the abscisic acid (ABA) signaling pathway, which can inhibit the activity of SnRK, leading to a decrease of the phosphorylation of its substrates in the signaling cascade [47]. Similarly, 14-3-3 protein availability fluctuation shows the drought condition and reported that drought stress can directly alter the abundance of 14-3-3 proteins [48].…”

Section: Protein Metabolism In Plant Roots and Shoots Due To Drought ...mentioning

confidence: 99%

Protein Metabolism in Plants to Survive against Abiotic Stress

Thapa¹,

Shrestha²

2022

Plant Defense Mechanisms

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Plants are frequently subjected to several abiotic environmental stresses under natural conditions causing profound impacts on agricultural yield and quality. Plants can themselves develop a wide variety of efficient mechanisms to respond environmental challenges. Tolerance and acclimation of plants are always related to significant changes in protein, cellular localization, posttranscription, and posttranslational modifications. Protein response pathways as well as pathways unique to a given stress condition shared by plants under different stressed environment are discussed in this chapter. The various signaling of protein such as fluctuation, overexpression, and silencing of the protein gene are observed to be modulated in drought-tolerant plants. Similarly, gene expression, RNA processing, and metabolic process take place to cope with drought conditions. For adaption in water-submerged conditions, plants undergo reactive oxygen species (ROS), cell wall modification, proteolysis, and post-recovery protein metabolism. Heat shock protein and protein and lipid contents vary and play pivotal role in resisting low and high temperatures. In a nutshell, this paper provides an overview of several modification, synthesis, degradation, and metabolism of protein in plants to cope with and revive again to normal growing conditions against abiotic stress, emphasizing drought, submerged, extreme cold, and heat temperatures.

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Morphological, Anatomical, and Molecular Consequences of Growth and Development at Low Temperature in Secale Cereale

Puma

Hüner

1985

American J of Botany

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Growth of winter cereals at low, nonfreezing temperatures is essential for the establishment ofa cold-hardy state and subsequent survival ofthe overwintering plant. In this paper, I describe the consequences of growth and development of Secale cereale L. cv. Puma at cold-hardening temperatures with respect to leaf morphology, anatomy, and biochemistry, and with particular emphasis on their relationship to photosynthetic acclimation. Low temperature-induced structural and functional alterations at the level of ribulose bisphosphate carboxylase-oxygenase and the chloroplast thylakoid membrane are described and related to overall photosynthetic efficiency and capacity for CO 2 utilization. Growth and development at cold-hardening temperatures appear to result in changes in protein conformation and membrane organization, but not in basic composition. It is proposed that developmental temperature imparts a significant effect on the assembly of these multimeric, photosynthetic components, which leads to distinct structural and functional changes.IN GENERAL, research in the area of plant coldhardiness can be divided into two principal I

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Differential Subunit Aggregation of a Purified Protein from Cold-hardened and Unhardened Puma Rye

Cited by 9 publications

References 12 publications

Long-term chilling of young tomato plants under low light

Long-term chilling of young tomato plants under low light

Protein Metabolism in Plants to Survive against Abiotic Stress

Morphological, Anatomical, and Molecular Consequences of Growth and Development at Low Temperature in Secale Cereale

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