Differential survival of Escherichia coli uvrA, uvrB, and uvrC mutants to psoralen plus UV-A (PUVA): Evidence for uncoupled action of nucleotide excision repair to process DNA adducts

Lage, Claudia; Gonçalves, Silvia R.F.; Souza, Luciana L.; Pádula, Marcelo de; Leitão, Álvaro C.

doi:10.1016/j.jphotobiol.2009.11.001

Cited by 12 publications

(10 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The differences between our results and those of Lage et al (27) could either be due to the strain backgrounds, the alleles used, or the experimental conditions, all of which differed significantly. Previous studies have reported that various strains of E. coli can vary significantly in their sensitivity to DNA cross-links (54).…”

Section: Resultscontrasting

confidence: 96%

“…While these results are consistent with most studies in the literature, they differ with one report by Lage et al (27), which showed that a uvrB5 mutant was severely sensitive to psoraleninduced DNA damage, whereas uvrA6 and uvrC34 mutants were nearly as resistant as their parental strain (27). The differences between our results and those of Lage et al (27) could either be due to the strain backgrounds, the alleles used, or the experimental conditions, all of which differed significantly.…”

Section: Resultssupporting

confidence: 75%

“…Further, the uvr mutants used in the Lage et al study were direct isolates from nitrous acidmutagenized cultures (55,56), making it possible that secondary mutations occurred in these strains. Finally, whereas we treated cultures with 10 g/ml 8-methoxypsoralen and used short UV-A exposure times, the Lage et al study (27) treated cultures with 1,000-fold lower psoralen concentrations and then used long UV-A exposure times to achieve lethal levels of DNA interstrand cross-links. This could potentially lead to growth or UV-A-specific effects in cultures during the irradiation period that may account for our observed differences.…”

Section: Resultsmentioning

confidence: 99%

“…In order to differentiate between these possibilities, we repeated our survival assays with the strains used in the Lage et al study (27). As shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

“…However, a recent study reported that uvrB mutants were more sensitive to psoralen-induced damage than either uvrA or uvrC, suggesting a potentially unique mechanism of repair for these lesions (27,28). While this represents a potentially important observation, it also appears to conflict with a number of earlier studies that suggest uvrA and uvrB mutants are equally sensitive to psoralen-induced DNA damage and are required for incisions to occur (23,29,30).…”

mentioning

confidence: 88%

See 4 more Smart Citations

Cho Endonuclease Functions during DNA Interstrand Cross-Link Repair in Escherichia coli

et al. 2016

View full text Add to dashboard Cite

DNA interstrand cross-links are complex lesions that covalently link both strands of the duplex DNA. Lesion removal is proposed to be initiated via the UvrABC nucleotide excision repair complex; however, less is known about the subsequent steps of this complex repair pathway. In this study, we characterized the contribution of nucleotide excision repair mutants to survival in the presence of psoralen-induced damage. Unexpectedly, we observed that the nucleotide excision repair mutants exhibit differential sensitivity to psoralen-induced damage, with uvrC mutants being less sensitive than either uvrA or uvrB. We show that Cho, an alternative endonuclease, acts with UvrAB and is responsible for the reduced hypersensitivity of uvrC mutants. We find that Cho's contribution to survival correlates with the presence of DNA interstrand cross-links, rather than monoadducts, and operates at a step after, or independently from, the initial incision during the global repair of psoralen DNA adducts from the genome. IMPORTANCEDNA interstrand cross-links are complex lesions that covalently bind to both strands of the duplex DNA and whose mechanism of repair remains poorly understood. In this study, we show that Cho, an alternative endonuclease, acts with UvrAB and participates in the repair of DNA interstrand cross-links formed in the presence of photoactivated psoralens. Cho's contribution to survival correlates with the presence of DNA interstrand cross-links and operates at a step after, or independently from, the initial incision during the repair process. P soralens are tricyclic asymmetrical compounds containing furan and pyrone rings and bind DNA nonspecifically, with a preference for pyrimidines to form noncovalent bonds (1-3). Upon absorption of UV-A light, a covalent bond forms through photoaddition between the C-5AC-6 double bond of the pyrimidine and the C-4=AC-5= furan double bond or C-3=AC-4= pyrone double bond of psoralen. Absorption of a second photon results in photoaddition on the remaining furan or pyrone with a second pyrimidine, creating a DNA interstrand cross-link. Thus, psoralen-induced damage consists of both monoadducts and DNA interstrand cross-links. Because DNA interstrand crosslinks covalently bind both strands of the duplex DNA, they inhibit cellular processes that require strand denaturation, including transcription and replication. This inhibition is generally considered to be the reason for the potency of psoralens, and this class of compounds is used in treating different skin diseases, such as vitiligo, psoriasis, and as a chemotherapeutic for some forms of cancer (4-6).Several models have been proposed for DNA interstrand crosslink repair. A feature common to most models is that the repair process is initiated by nucleotide excision repair, followed by the sequential action of other DNA repair processes, such as recombination or translesion synthesis, which function to provide an undamaged template that replaces the incised sequence. In these models, a second round of nucleotide exc...

show abstract

Section: Resultscontrasting

confidence: 96%

Section: Resultssupporting

confidence: 75%

Section: Resultsmentioning

confidence: 99%

“…In order to differentiate between these possibilities, we repeated our survival assays with the strains used in the Lage et al study (27). As shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 88%

See 3 more Smart Citations

Cho Endonuclease Functions during DNA Interstrand Cross-Link Repair in Escherichia coli

et al. 2016

View full text Add to dashboard Cite

show abstract

UvrA expression of Lactococcus lactis NZ9000 improve multiple stresses tolerance and fermentation of lactic acid against salt stress

Moghaddam

Zhang

2017

J Food Sci Technol

View full text Add to dashboard Cite

Lactococcus lactis is subjected to several stressful conditions during industrial fermentation including oxidation, heating and cooling, acid, high osmolarity/ dehydration and starvation. DNA lesion is a major cause of genetic instability in L. lactis that usually occurs at a low frequency, but it is greatly enhanced by environmental stresses. DNA damages produced by these environmental stresses are thought to induce DNA double-strand breaks, leading to illegitimate recombination. Nucleotide excision repair (NER) protein UvrA suppresses multiple stressesinduced illegitimate recombination. UvrA protein can survive a coincident condition of environmental harsh conditions, multiple stress factors supposedly encountered in the host and inducing UvrA in L. lactis. In this study the expression of UvrA and growth performance and viability of control strain L. lactisVector and recombinant strain L. lactisUvrA under multiple stress conditions were determined. The recombinants strain had 30.70 and 52.67% higher growth performances when subjected to acidic and osmotic stresses conditions. In addition, the L. lactisUvrA strain showed 1.85-, 1.65-, and 2.40-fold higher biomass, lactate production, and lactate productivity, compared with the corresponding values for L. lactisVector strain during the osmotic stress. Results demonstrated NER system is involved in adaptation to various stress conditions and suggested that cells with a compromised UvrA as DNA repair system have an enhanced protection behavior in L. lactis NZ9000 against DNA damage.

show abstract

Evidence for involvement of UvrB in elicitation of ‘SIR’ phenotype by rpoB87-gyrA87 mutations in lexA3 mutant of Escherichia coli

Shanmughapriya

Munavar

2012

DNA Repair

View full text Add to dashboard Cite

Differential survival of Escherichia coli uvrA, uvrB, and uvrC mutants to psoralen plus UV-A (PUVA): Evidence for uncoupled action of nucleotide excision repair to process DNA adducts

Cited by 12 publications

References 49 publications

Cho Endonuclease Functions during DNA Interstrand Cross-Link Repair in Escherichia coli

Cho Endonuclease Functions during DNA Interstrand Cross-Link Repair in Escherichia coli

UvrA expression of Lactococcus lactis NZ9000 improve multiple stresses tolerance and fermentation of lactic acid against salt stress

Evidence for involvement of UvrB in elicitation of ‘SIR’ phenotype by rpoB87-gyrA87 mutations in lexA3 mutant of Escherichia coli

Contact Info

Product

Resources

About