Differential susceptibility of primary cultured human skin cells to hypericin PDT in an in vitro model

Popović, Aleksandra; Wiggins, T.; Davids, Lester M.

doi:10.1016/j.jphotobiol.2015.06.009

Cited by 24 publications

(10 citation statements)

References 59 publications

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“…Other results indicate a cytoplasmic localization of hypericin in all investigated skin cell types whereas the intracellular generated ROS were the most elevated in fibroblasts. This study describes the effects induced by in vitro PDT using hypericin on different human skin cells, gathering hence data on PS efficacy that could impacts in vivo application for NMSC [87].…”

Section: In Vitro Models For Nonmelanoma Skin Cancer Pdt Therapymentioning

confidence: 99%

Photosensitizers Imprinting Intracellular Signaling Pathways in Dermato-Oncology Therapy

Constantin¹,

Neagu²

2017

Photomedicine - Advances in Clinical Practice

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This chapter describes the main deregulated intracellular pathways at both genetic and proteomic levels that are found in three main skin cancers: basal cell carcinoma, squamous cell carcinoma and melanoma. In basal cell carcinoma, the main intracellular signaling pathways is the Sonic Hedgehog pathway, while in squamous cell carcinoma, it is the p53 pathway. However, in both nonmelanoma skin cancers, these major pathways trigger cross-activation with other important ones. In melanoma, mitogen-activated protein kinase pathway and PI3K/Akt pathways are deeply deregulated, and moreover due to the disease complexity, BRAF, RAS (N/H/K), NF1 and Triple-WT melanoma subtypes need additional molecular stratification. The stage in which photodynamic therapies' clinical application is in the treatment of these diseases is another subject tackled by the chapter. Thus, if basal cell carcinoma and squamous cell carcinoma possess in their therapeutical armamentarium photodynamic therapies approach, melanoma, with its particularities, still needs thorough molecular investigations to adapt this particular therapy. Based on the accumulated knowledge on pathological intracellular pathways, the chapter describes the molecular details that reside in applying photodynamic therapy. In vivo and in vitro models of cutaneous malignancy and photodynamic therapies' molecular events are further detailed.

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Section: In Vitro Models For Nonmelanoma Skin Cancer Pdt Therapymentioning

confidence: 99%

Photosensitizers Imprinting Intracellular Signaling Pathways in Dermato-Oncology Therapy

Constantin¹,

Neagu²

2017

Photomedicine - Advances in Clinical Practice

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“…Besides molecular oxygen and light, PDT mainly relies on PS’s passive accumulation in the diseased tissues to achieve its best therapeutic effect [ 140 ]. Unfortunately, PDT can adversely damage healthy tissues, when the PS non-specifically accumulates in the latter [ 73 , 141 , 142 ]. PDT efficacy can be affected by multiple factors including light accessibility to the tumor site and hypoxia [ 143 , 144 , 145 ].…”

Section: Introductionmentioning

confidence: 99%

Antibody-Based Immunotherapy: Alternative Approaches for the Treatment of Metastatic Melanoma

et al. 2020

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Melanoma is the least common form of skin cancer and is associated with the highest mortality. Where melanoma is mostly unresponsive to conventional therapies (e.g., chemotherapy), BRAF inhibitor treatment has shown improved therapeutic outcomes. Photodynamic therapy (PDT) relies on a light-activated compound to produce death-inducing amounts of reactive oxygen species (ROS). Their capacity to selectively accumulate in tumor cells has been confirmed in melanoma treatment with some encouraging results. However, this treatment approach has not reached clinical fruition for melanoma due to major limitations associated with the development of resistance and subsequent side effects. These adverse effects might be bypassed by immunotherapy in the form of antibody–drug conjugates (ADCs) relying on the ability of monoclonal antibodies (mAbs) to target specific tumor-associated antigens (TAAs) and to be used as carriers to specifically deliver cytotoxic warheads into corresponding tumor cells. Of late, the continued refinement of ADC therapeutic efficacy has given rise to photoimmunotherapy (PIT) (a light-sensitive compound conjugated to mAbs), which by virtue of requiring light activation only exerts its toxic effect on light-irradiated cells. As such, this review aims to highlight the potential clinical benefits of various armed antibody-based immunotherapies, including PDT, as alternative approaches for the treatment of metastatic melanoma.

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“…Photodynamic therapy (PDT) [1–3] is an established type of therapy affected by light source, photosensitizer, and molecular oxygen [4], which can produce reactive oxygen species (ROS)-mediated [5, 6] direct lethal effects on cancer cells within the illuminated area under the condition of minimally invasive nature [6] and low toxicity. It can give rise to DNA damage [7], activate signaling pathways to facilitate vasculotoxic reaction that interdicts blood supply to the tumor area [8], and provoke tumor cells recognition and destruction by the immune system [9].…”

Section: Introductionmentioning

confidence: 99%

Protoporphyrin IX-loaded laminarin nanoparticles for anticancer treatment, their cellular behavior, ROS detection, and animal studies

Wang

Guo

et al. 2019

Nanoscale Res Lett

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Laminarin conjugate-based nano-scaled particles were in this study proposed as a delivery system for protoporphyrin IX (Pp IX) in photodynamic therapy (PDT) of human breast cancer cells (MCF-7). Hematin-Laminarin-Dithiodipropionic Acid-MGK, named as HLDM, was an amphiphilic carrier material with dual pH/redox sensitive that could be used to load hydrophobic drug to improve their solubility and enhance biocompatibility. Therefore, we combined photosensitizer (Pp IX) with HLDM to fabricate a novel nano-micelles, herein called Pp IX-loaded HLDM micelles. The Pp IX-loaded HLDM micelles were 149.3 ± 35 nm sized in neutral water. Phototoxicity, in vitro PDT effect, and dual sensibility to pH and redox microenvironment of Pp IX-loaded HLDM micelles were examined at different concentrations by using MCF-7 human breast cancer cells. The experiments on phototoxicity and reactive oxygen species (ROS) production proved that the micelles could produce PDT to kill the cancer cells with a certain wavelength light. The apoptosis experiment indicated that the micelles could cause nuclear damage. In vivo PDT effect of the micelles was studied by constructing the tumor-bearing nude mouse model of MCF-7 cells. In vivo studies showed that the Pp IX-loaded HLDM micelles could induce remarkable anti-tumor effect. A promising laminarin-based nanomedicine platform acts as a new drug delivery system to enhance the uptake, accumulation, and PDT efficacy of Pp IX in vitro and in vivo.

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Differential susceptibility of primary cultured human skin cells to hypericin PDT in an in vitro model

Cited by 24 publications

References 59 publications

Photosensitizers Imprinting Intracellular Signaling Pathways in Dermato-Oncology Therapy

Photosensitizers Imprinting Intracellular Signaling Pathways in Dermato-Oncology Therapy

Antibody-Based Immunotherapy: Alternative Approaches for the Treatment of Metastatic Melanoma

Protoporphyrin IX-loaded laminarin nanoparticles for anticancer treatment, their cellular behavior, ROS detection, and animal studies

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