DIFFERENTIAL TECHNIQUES AND METHODS OF ISOLATION OF <i>PSEUDOMONAS</i>

Klinge, K

doi:10.1111/j.1365-2672.1960.tb00216.x

Cited by 31 publications

(16 citation statements)

References 58 publications

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“…H202 (20 vol.) was used to test for catalase; the benzidine test was carried out by the method of Deibel & Evans (1960); oxidase was tested for by the method of Klinge (1960), strong oxidase-positive reactions being recorded after I o s and weak ones after 30 s to I min.…”

Section: Henry Technique (Irridescence)mentioning

confidence: 99%

A Numerical Taxonomic Study of Coryneform and Related Bacteria

Jones¹

1975

Journal of General Microbiology

168

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S U M M A R YTwo hundred and thirty-three strains of coryneform bacteria, including representatives of the genera Arthrobacter, Brevibacterium, Cellulomonas, Corynebacterium, Erysipelothrix, Jensenia, Kurthia, Listeria, Microbacterium, Mycobacterium, Nocardia and Propionibacterium and other related bacteria, were studied using I 73 morphological, physiological and biochemical tests. The bacteria were grown on a soil extract medium which allowed growth of all the strains, and all were incubated at 30 "C. The results were subjected to computer analysis. The majority of the Arthrobacter is a large loose taxon and it is premature to decide on its taxonomic rank. The genus Brevibacterium should be retained for B. linens and closely related strains. (v) The cellulolytic forms of Nocardia should be removed from the genus ; they are however quite distinct from Cellulomonas.

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Section: Henry Technique (Irridescence)mentioning

confidence: 99%

A Numerical Taxonomic Study of Coryneform and Related Bacteria

Jones¹

1975

Journal of General Microbiology

168

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“…All oxidized glucose, were oxidase positive, penicillin resistant and produced pyoverdine (fluorescin) but not pyocyanine on the appropriate media proposed by King, Ward & Raney (1954). Nine distinct bio-groups had been recognized among 30 of the isolates on the basis of more than 70 biochemical features (Stewart, 1964) ; by using the criteria proposed by Klinge (1959Klinge ( , 1960, 24 of these 30 isolates were assignable to P. putida and the other 6 to P. '$uorescens Glucose was chosen in preference to galactose, which was used by Rhodes (1959), because manometric studies showed that glucose was more rapidly oxidized by the isolates. The buffer salt (K,HPO,) was kept at a low concentration to allow a more rapid increase in pH value with base production.…”

Section: Methodsmentioning

confidence: 99%

The Urease Activity of Fluorescent Pseudomonads

Stewart

1965

Journal of General Microbiology

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SUMMARYMedia frequently used for the detection of urease activity were found to be unsuitable for fluorescent pseudomonads since the presence of free ammonia was found to suppress the formation of urease in growing cultures. Incubation of cultures for 40 hr at 25" in a low concentration Casitone+ yeast-extract +glucose medium, followed by the addition of urea to 0.2 yo (w/v), resulted in the development of an alkaline shift in the medium after further incubation for a few more hours at 37". This method gave good results in the detection of urease activity.

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“…For the experiments P. fluorescens DSM 4358 (DSMZ, Braunschweig, Germany), a typical heterotrophic soil bacterium was used, which can grow aerobically by oxygen respiration or anoxically by nitrate respiration [36]. P. fluorescens was grown in medium M535 (Merck, Darmstadt, Germany), containing 17 g peptone from casein, 3 g peptone from tryptically digested soy meal, 2.5 g D(+)-glucose monohydrate, 5 g NaCl and 2.5 g di-potassium hydrogen phosphate per L at a pH of 7.3 +/-0.2.…”

Section: Cultivation Of P Fluorescensmentioning

confidence: 99%

Section: Stainless Steel Container (Ssc)mentioning

confidence: 99%

“…P. fluorescens was an ideal microorganism for long-term aseptic, but non-sterile lab experiments, since it had a higher affinity for nitrate and easily degradable carbon sources than other soil bacteria that might compete in an anoxic environment. It could grow under aerobic, anoxic and anaerobic conditions as well [36], [42] and thus was used as a versatile microorganism to test the microbial growth behavior in/on soil and groundwater.During aerobic incubation with non-restricted oxygen supply approximately 34 % of the DOC of the M535 medium was consumed in 24 h. The dry weight of aerobically grown cells in a shake culture was 3.1 g/L, resulting in a yield of 0.93 +/-0.02 g dry biomass per g DOC (1g DOC is represented by approximately 2.5 g organic substances in M535) consumed at surplus substrate supply. Hence for growth within the CF in quartz sand, where the oxygen supply is restricted, a much lower yield can be assumed.…”

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confidence: 99%

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Influence of Increasing Concentrations of Organic Pollutants on Biofilm Formation and Flow Parameters in a Sand-aquifer and Its Capillary Fringe.

Jost¹,

Winter²,

Gallert³

2017

EPP

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Abstract. We followed the development of a Pseudomonas fluorescens biofilm on quartz sand in an instrumented horizontal flow-through stainless steel container (SSC) in lysimeter-scale, to investigate its impact on physical soil parameters within the aquifer and the capillary fringe (CF). Increasingly concentrated biodegradable substances in a liquid medium were pumped through the sand in the SSC creating an artificial aquifer. Biofilm development and its influence on flow parameters and oxygen concentration profiles, as well as temperature or water suction changes with time were determined. After more than 19 weeks of medium flow, at DOC concentrations of finally about 300 mg/L, P. fluorescens formed a strong biofilm and the highest biomass concentrations with up to 2.8 mg volatile solids per g dry sand were found in the transition zone of the CF. The soil temperature, which was slightly increased, or the water suction was significantly influenced during the experiment. The effects were strongest within the first 0.3 m of flow stretch, where the highest biomass values were detected. During the total experimental duration of 54 weeks the average flow velocity was reduced by up to 15 % due to clogging effects and the oxygen profiles were changed drastically. Results may be representative for real polluted aquifers. Keywords:Pseudomonas fluorescens, sand aquifer, capillary fringe, soil contamination, biofilm formation, biological clogging. IntroductionSoil and groundwater are naturally inhabited by a vast variety of prokaryotes. It was estimated that 2.5 x 10 29 microorganisms live in the top 8 m of the terrestrial subsurface and at least 2.5 x 10 30 microorganisms below 8 m depth, either suspended in the water phase or attached onto organic and mineral compounds of top soil and the underground [53]. Anaerobic bacteria in deep soil layers may ferment organic pollutants to mainly fatty acids whereas aerobic bacteria in upper soil layers may respire organic pollutants in the presence of oxygen to carbon dioxide and thus contribute to "clean-up". There seems to be a spatial heterogeneity of microorganisms. Whereas mainly aerobic bacteria, fungi and protozoa colonize soil surface layers, the proportion of mainly anaerobic bacteria (most of them are "fermenters") and of Actinomycetes increases with depth in the underground in the vicinity and below the groundwater table. These population changes are accompanied by a significantly decreasing diversity of microorganisms [17] and by a decrease of physiological functions [45]. Groundwater bacteria tend to form a biofilm on mineral surfaces of soil particles and may cause preferential flow paths or clogging in heterogeneous soil compartments [45]. In contrast aerobic motile bacteria can even move into the top zone of the capillary fringe (CF, spanning from the groundwater table to still visible moisture above it in a quartz sand aquifer) above the water-saturated zone of quartz sand and form a dense biofilm in the still highly saturated interface region of the CF [31]. Biofi...

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DIFFERENTIAL TECHNIQUES AND METHODS OF ISOLATION OF PSEUDOMONAS

Cited by 31 publications

References 58 publications

A Numerical Taxonomic Study of Coryneform and Related Bacteria

A Numerical Taxonomic Study of Coryneform and Related Bacteria

The Urease Activity of Fluorescent Pseudomonads

Influence of Increasing Concentrations of Organic Pollutants on Biofilm Formation and Flow Parameters in a Sand-aquifer and Its Capillary Fringe.

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