Differential Toxicities of Nickel Salts to the Nematode Caenorhabditis elegans

Meyer, Dean; Birdsey, Jennifer M.; Wendolowski, Mark A.; Dobbin, Kevin K.; Williams, Phillip L.

doi:10.1007/s00128-016-1846-z

Cited by 7 publications

(2 citation statements)

References 12 publications

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“…The current result supports our earlier reports where we have shown that Ni induces lethality in developing C. elegans at very low concentrations [16]. Other studies have also corroborated that Ni exposure reduces C. elegans survival from larval stages to adults [28][29][30]. Similarly, other studies have established Ni toxicities in both human and vertebrate animal models, particularly its neurotoxic effects [31,32].…”

Section: Discussionsupporting

confidence: 92%

p38- and ERK-MAPK Signalling Modulate Developmental Neurotoxicity of Nickel and Vanadium in the Caenorhabditis elegans Model

Ijomone,

Weishaupt,

Michaelis

et al. 2024

Kinases and Phosphatases

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Nickel (Ni) and vanadium (V) are characteristic heavy metal constituents of many crude oil blends in Sub-Saharan Africa, and we have previously demonstrated their neurotoxic impact. However, molecular mechanisms driving Ni and V neurotoxicity are still being elucidated. The p38- and ERKs-MAPK pathways, which are mostly known for their involvement in human immune and inflammatory signalling, have been shown to influence an array of neurodevelopmental processes. In the present study, we attempt to elucidate the role of p38- and ERK-MAPK in neurotoxicity after early life exposures to Ni and V using the Caenorhabditis elegans model. Synchronized larvae stage-1 (L1) worms were treated with varying concentrations of Ni and V singly or in combination for 1 h. Our results show Ni induces lethality in C. elegans even at very low concentrations, while much higher V concentrations are required to induce lethality. Furthermore, we identified that loss-of-function of pmk-1 and pmk-3, which are both homologous to human p38-α (MAPK14), is differentially affected by Ni and V exposures. Also, all exposure scenarios triggered significant developmental delays in both wild-type and mutant strains. We also see increased mitochondrial-derived reactive oxygen species following Ni and V exposures in wild-type worms with differential responses in the mutant strains. Additionally, we observed alterations in dopamine and serotonin levels after metal exposures, particularly in the pmk-1 strain. In conclusion, both Ni and V induce lethality, developmental delays, and mitochondrial-derived ROS in worms, with V requiring a much higher concentration. Further, the results suggest the p38- and ERK-MAPK signalling pathways may modulate Ni and V neurodevelopmental toxicity, potentially affecting mitochondrial health, metal bioavailability, and neurotransmitter levels.

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Section: Discussionsupporting

confidence: 92%

p38- and ERK-MAPK Signalling Modulate Developmental Neurotoxicity of Nickel and Vanadium in the Caenorhabditis elegans Model

Ijomone,

Weishaupt,

Michaelis

et al. 2024

Kinases and Phosphatases

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“…For a long time, different toxicity tests with metals and metallic salts have been carried out [46,47], proving to be a predictor of acute lethality in mammals, generating LC50 values parallel to LD50 values for the rat and mouse, since LC50 values were compared to the oral LD50 values published in mammals for the salts of the same metals [48,49].…”

Section: Figure 2 Percentage Of Individual Alive (C Elegans) Versus B...mentioning

confidence: 99%

Antioxidant activity and cytotoxicity of Vacciniun corymbosum L. cultivated in Tucumán, Argentina

Lorenzi¹,

Ale²,

Moyano³

2022

World J. Adv. Res. Rev.

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Among berries, blueberries (Vaccinium corymbosum L.) are considered one of the main sources of phenolic compounds and are appreciated for their high antioxidant activity. Due to the apparent relationship between phytochemicals in plants to the prevention of chronic diseases, the study of the content and physiological activity of phenolic compounds and other bioactive metabolites present in blueberries is interesting. In this work, Tucuman blueberries of the O'Neal variety were used, out of which four different extracts were obtained. One of them was obtained by sequential leaching with hydroalcoholic mixtures from frozen fresh fruits (E1), a second extract from aqueous leaching (E2), and acetone as solvent (E3). Spectrophotometric techniques were performed to quantify Total Phenols, Total Flavonoids and Anthocyanins. The extracts presented a similar quantitative profile of phenolic compounds. The antioxidant activity of the extracts obtained was evaluated using two different techniques (ABTS•+ and DPPH•). The extracts showed a free radical inhibitory activity and a similar reducing power. Through the acute toxicity test (24h) of the organic and aqueous extracts from the blueberry on C. elegans, .it was possible to determine that the extracts under study do not show toxicity for further study.

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Definitive Assessment of the Level of Risk of Exhausted Catalysts: Characterization of Ni and V Contaminates at the Limit of Detection

et al. 2018

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Differential Toxicities of Nickel Salts to the Nematode Caenorhabditis elegans

Cited by 7 publications

References 12 publications

p38- and ERK-MAPK Signalling Modulate Developmental Neurotoxicity of Nickel and Vanadium in the Caenorhabditis elegans Model

p38- and ERK-MAPK Signalling Modulate Developmental Neurotoxicity of Nickel and Vanadium in the Caenorhabditis elegans Model

Antioxidant activity and cytotoxicity of Vacciniun corymbosum L. cultivated in Tucumán, Argentina

Definitive Assessment of the Level of Risk of Exhausted Catalysts: Characterization of Ni and V Contaminates at the Limit of Detection

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