Differential transcription of Pgk genes during spermatogenesis in the mouse

McCarrey, John R.; Berg, Werner M.; Paragioudakis, Steve J.; Zhang, Peter L.; Dilworth, Donald D.; Arnold, Brent L.; Rossi, John J.

doi:10.1016/0012-1606(92)90056-m

Cited by 146 publications

(126 citation statements)

References 23 publications

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“…In the absence of any significant cytoplasm and the replacement of histones for protamines, how might epigenetic information be transmitted from the sperm to the zygote? Previous studies of X-linked genes silenced by MSCI in eutherians have shown that hypermethylation of DNA is not involved (54,55). Studies of the active and inactive X-chromosomes in female kangaroos have indicated that DNA methylation is also not involved in somatic XCI (56).…”

Section: Resultsmentioning

confidence: 95%

Sex chromosome silencing in the marsupial male germ line

Namekawa

VandeBerg

McCarrey

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

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In marsupials, dosage compensation involves silencing of the father's X-chromosome. Because no XIST orthologue has been found, how imprinted X-inactivation occurs is unknown. In eutherians, the X is subject to meiotic sex chromosome inactivation (MSCI) in the paternal germ line and persists thereafter as postmeiotic sex chromatin (PMSC). One hypothesis proposes that the paternal X is inherited by the eutherian zygote as a preinactive X and raises the possibility of a similar process in the marsupial germ line. Here we demonstrate that MSCI and PMSC occur in the opossum. Surprisingly, silencing occurs before X-Y association. After MSCI, the X and Y fuse through a dense plate without obvious synapsis. Significantly, sex chromosome silencing continues after meiosis, with the opossum PMSC sharing features of eutherian PMSC. These results reveal a common gametogenic program in two diverse clades of mammals and support the idea that male germ-line silencing may have provided an ancestral form of mammalian dosage compensation.meiosis ͉ X-inactivation

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Section: Resultsmentioning

confidence: 95%

Sex chromosome silencing in the marsupial male germ line

Namekawa

VandeBerg

McCarrey

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

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“…Presence of SSC was confiremd by expression of integrin a 6 and integrin b 1 by immunohistochemistry, which are cell surface markers for SSC. BMS-GC cells were negative for synaptonemal complex protein 3, 26 phosphoglycerate kinase 2 (Pgk2), 27 acrosin 28 and transition protein 2, 28 which are markers for meiotic and postmeiotic germ cells (data not shown). This is an indication for differentiation arrest of BSC-derived germ cells at the premeiotic stages in vitro.…”

Section: Resultsmentioning

confidence: 98%

Derivation of male germ cells from bone marrow stem cells

Nayernia

Lee

Drusenheimer

et al. 2006

Laboratory Investigation

286

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Recent studies have demonstrated that somatic stem cells have a more flexible potential than expected, whether put into tissue or cultured under different conditions. Bone marrow (BM)-derived stem cells can transdifferentiate into multilineage cells, such as muscle of mesoderm, lung and liver of endoderm, and brain and skin of ectoderm origin. Here we show that BM stem cells are able to transdifferentiate into male germ cells. For derivation of male germ cells from adult BM stem (BMS) cells, we used the Stra8-enhanced green fluoresence protein (EGFP) transgenic mouse line expressing EGFP specifically in male germ cells. BMS cellderived germ cells expressed the known molecular markers of primordial germ cells, such as fragilis, stella, Rnf17, Mvh and Oct4; as well as molecular markers of spermatogonial stem cells and spermatogonia including Rbm, c-Kit, Tex18, Stra8, Piwil2, Dazl, Hsp90a, b1-and a6-integrins. Our ability to derive male germ cells from BMS cells reveals novel aspects of germ cell development and opens the possibilities for use of these cells in reproductive medicine.

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“…Germ cells were isolated from each individual male mouse by using a 50-mL "mini" Sta-Put chamber as described (27). Gonadal tissue was subjected to sequential digestions of 15 min each at 37 8C with 0.5 mg/mL collagenase and 0.5 mg/mL trypsin.…”

Section: Methodsmentioning

confidence: 99%

Primary epimutations introduced during intracytoplasmic sperm injection (ICSI) are corrected by germline-specific epigenetic reprogramming

Waal

Yamazaki

Ingale

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

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The use of assisted reproductive technologies (ART) has become increasingly common worldwide and is now responsible for 2-3% of children born in developed countries. Multiple reports have suggested that ART-conceived children are more likely to develop rare epigenetic disorders such as Beckwith-Wiedemann Syndrome or Angelman Syndrome, both of which involve dysregulation of imprinted genes. Anecdotal reports suggest that animals produced with ART that manifest apparent epigenetic defects typically do not transmit these epimutations to subsequent generations when allowed to breed naturally, but this hypothesis has not been directly studied. We analyzed allele-specific DNA methylation and expression at three imprinted genes, H19, Snrpn, and Peg3, in somatic cells from adult mice generated with the use of intracytoplasmic sperm injection (ICSI), a type of ART. Epimutations were detected in most of the ICSI-derived mice, but not in somatic cells of their offspring produced by natural mating. We examined germ cells from the ICSI mice that exhibited epimutations in their somatic cells and confirmed normal epigenetic reprogramming of the three imprinted genes analyzed. Collectively, these results confirm that ART procedures can lead to the formation of primary epimutations, but while such epimutations are likely to be maintained indefinitely in somatic cells of the ART-derived individuals, they are normally corrected in the germ line by epigenetic reprogramming and thus, not propagated to subsequent generations.gametogenesis | transgenerational inheritance E arly embryos and germ cells are unique in that they must undergo extensive epigenetic reprogramming to reestablish developmental potency during each generation. This reprogramming entails erasure of most inherited epigenetic modifications followed by the acquisition and subsequent maintenance of new epigenetic profiles (1). Genomic imprinting is an epigenetic phenomenon found in eutherian and metatherian mammals (2) that results in parent-of-origin-specific, monoallelic expression of a subset of genes (3). This functional asymmetry of imprinted genes is conferred through differential DNA methylation patterns established during gametogenesis in each parent that distinguish the maternal and paternal alleles in the ensuing offspring (4, 5). These regions are known as differentially methylated regions (DMRs), and the differential methylation profiles at these genetic elements play an important role in regulating allele-specific expression of imprinted genes (3).Because the epigenome is distinct in each cell type and is readily reversible by developmental reprogramming, it is particularly susceptible to disruption by environmental influences (6). Such epigenetic defects are known as "epimutations" and can be of two types-primary epimutations or secondary epimutations (7). Primary epimutations result from a direct disruption of an epigenetic parameter such as DNA methylation that is then propagated through DNA replication to subsequent cells. Secondary epimutations result...

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Differential transcription of Pgk genes during spermatogenesis in the mouse

Cited by 146 publications

References 23 publications

Sex chromosome silencing in the marsupial male germ line

Sex chromosome silencing in the marsupial male germ line

Derivation of male germ cells from bone marrow stem cells

Primary epimutations introduced during intracytoplasmic sperm injection (ICSI) are corrected by germline-specific epigenetic reprogramming

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