Differential Transcription Start Site Selection Within Core Promoters Has Implications on the Level of Protein Expressed

Native RNA G quadruplex immunoprecipitation (rG4IP) from mammalian cells

“…Furthermore, we have also detected the enrichment of AGAP2 mRNA in the BG4 antibody fraction, supporting our hypothesis that this mRNA does contain G quadruplex structures in vivo . And by using our plasmid transfection approach, we have confirmed those structures’ location in the 5′UTR and revalidated the efficiency of our technique to pull down mRNAs containing G quadruplex structures ( Figure 3 B and see also troubleshooting 5 ) ( Surani et al, 2022 ). Our technique, which involves no fixation steps, validates the formation of these structures inside the cell and, if coupled with RNA sequencing, can be used to identify mRNAs with G quadruplex structures, and expand our knowledge of these structures on key cellular processes and pathophysiological conditions.…”