Differential transcriptional activity of SAD, FAD2 and FAD3 desaturase genes in developing seeds of linseed contributes to varietal variation in α-linolenic acid content

Rajwade, Ashwini V.; Kadoo, Narendra Y.; Borikar, Sanjay P.; Harsulkar, Abhay; Ghorpade, P. B.; Gupta, Vidya S.

doi:10.1016/j.phytochem.2013.12.002

Cited by 41 publications

(51 citation statements)

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“…We previously reported nucleotide sequences and expression patterns of the three desaturase genes (and their isoforms) SAD (SAD1 and SAD2), FAD2 (FAD2 and FAD2-2) and FAD3 (FAD3A and FAD3B) from ten Indian linseed varieties making two groups with varying ALA contents. 35 The results for the expression analysis of desaturase genes were in agreement with the FA data for the groups based on their ALA contents, where the high-ALA group showed higher expression of all the desaturase genes compared with that in the low-ALA group. 35 Of the six desaturase genes studied, three (FAD2-2, FAD3A and FAD3B) contributed significantly, while the remaining genes contributed either marginally (SAD1 and FAD2-1) or not at all (SAD2) to the ALA contents of the linseed varieties.…”

Section: Introductionsupporting

confidence: 81%

“…35 The results for the expression analysis of desaturase genes were in agreement with the FA data for the groups based on their ALA contents, where the high-ALA group showed higher expression of all the desaturase genes compared with that in the low-ALA group. 35 Of the six desaturase genes studied, three (FAD2-2, FAD3A and FAD3B) contributed significantly, while the remaining genes contributed either marginally (SAD1 and FAD2-1) or not at all (SAD2) to the ALA contents of the linseed varieties. These results suggested that accumulation of the final product ALA is not entirely dependent on the activity of a single desaturase gene but is a cumulative result of the activities of all three desaturases acting sequentially.…”

Section: Introductionsupporting

confidence: 81%

“…Six desaturase genes were isolated and sequenced from ten linseed varieties having varied FA composition (Table ) . The sequences were aligned with respective desaturase gene sequences from NCBI (http://www.ncbi.nlm.nih.gov/) and Phytozome (http://www.phytozome.net/) databases .…”

Section: Methodsmentioning

confidence: 99%

“…Six desaturase genes were isolated and sequenced from ten linseed varieties having varied FA composition ( Table 1). 35 The sequences were aligned with respective desaturase gene sequences from NCBI (http://www.ncbi.nlm.nih.gov/) and Phytozome (http://www.phytozome.net/) databases. 36 AA sequences of all six desaturases were deduced based on the gene sequence using MEGA v5.0, 37 and conserved motifs were determined in each case.…”

Section: Sequence Analysis Of Fad Genesmentioning

confidence: 99%

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Sequence characterization and in silico structure prediction of fatty acid desaturases in linseed varieties with differential fatty acid composition

et al. 2016

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Section: Introductionsupporting

confidence: 81%

Section: Introductionsupporting

confidence: 81%

Section: Methodsmentioning

confidence: 99%

Section: Sequence Analysis Of Fad Genesmentioning

confidence: 99%

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Sequence characterization and in silico structure prediction of fatty acid desaturases in linseed varieties with differential fatty acid composition

et al. 2016

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“…However, some oil seed plants failed to identify D8D, D5D, such as Sacha Inchi , tung tree (Galli et al 2014) and Yellow Horn ). There were four unigenes expressed over 100 RPKM, in which three (CL32923Contig1, CL27152Contig1 and CL21434Con-tig1) were annotated to SAD, and the remaining one (CL32549Contig1) to D12D, which are correlated with fatty acid composition and content (Zeng et al 2014), and corresponded to fast oil accumulation stage (Galli et al 2014;Wang et al 2012;Rajwade et al 2014). This was verified by unsaturated fatty acid content, in which C18:1 was the highest and followed by C18:2 Zhang et al 2015;Long et al 2012).…”

Section: Discussion Fatty Acids Biosynthesis and Accumulationmentioning

confidence: 99%

Transcriptome comparative analysis of two Camellia species reveals lipid metabolism during mature seed natural drying

Feng

Yang

Weiwei

et al. 2017

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Key message The molecular mechanisms of fatty acid biosynthesis and accumulation during Camellia meiocarpa and Camellia oleifera seed natural drying are characterized through transcriptome analyses. Abstract Camellia seed oil has been used as high quality and healthy food for over two thousand years. Seed drying affects oil quality and quantity; however, the molecular mechanisms of fatty acid biosynthesis and accumulation during the drying process remain unknown. In this study, the transcriptomes of Camellia meiocarpa and C. oleifera seed were characterized at five moisture content levels (10-50%) to identify the major processes and reveal genes affecting lipid metabolism in response to natural drying. We found a total of 111,156 unigenes by de novo assembled from RNA-Seq libraries of five moisture content levels during after-ripening of C. meiocarpa (74,016) and C. oleifera (76,374). Ten pathways were closely linked to changes in oil content and composition with 244 genes involved in fatty acid synthesis and accumulation. Gene ontology enrichment of differentially expressed genes (DEGs) indicated that fatty acid synthesis and accumulation are essential in C. meiocarpa while fatty acid accumulation in C. oleifera during natural drying process. Comparative analyses of DEGs between any two consecutive moisture contents identified six and three key unigenes in C. meiocarpa and C. oleifera seeds, respectively, and one additional unigene responsible for the difference between the two species' fatty acid synthesis and accumulation. Natural drying has improved the quality and quantity of the camellia seed oil. The study provided: (a) global transcriptional profiles at five moisture content levels during seed natural drying, (b) highlighting transcripts putatively involved in the regulation of gene expression program and in specific processes likely essential for lipid metabolism, and (c) discovery of genes associated with oil seed quantity and quality improvement for the studied two camellia species.Communicated by M. Buckeridge. Electronic supplementary materialThe online version of this article

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Transcriptomics of developing wild sunflower seeds from the extreme ends of a latitudinal gradient differing in seed oil composition

et al. 2022

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Seed oil composition, an important agronomic trait in cultivated sunflower, varies latitudinally across the native range of its wild progenitor. This pattern is thought to be driven by selection for a higher proportion of saturated fatty acids in southern populations compared with northern populations, likely due to the different temperatures experienced during seed germination. To investigate whether these differences in fatty acid composition between northern and southern populations correspond to transcriptional variation in the expression of genes involved in fatty acid metabolism, we sequenced RNA from developing seeds of sunflowers from Texas, USA, and Saskatchewan, Canada (the extreme ends of sunflower's latitudinal range) grown in a common garden. We found 4,741 genes to be differentially expressed between Texas and Canada, including several genes involved in lipid metabolism. Several differentially expressed lipid metabolism genes also colocalized with known oil quantitative trait loci (QTL). The genes producing stearoyl‐ACP‐desaturases ( SAD ) were of particular interest because of their known role in the conversion of fully saturated into unsaturated fatty acids. Two SAD genes were more highly expressed in seeds from Canadian populations, consistent with the observation of increased levels of unsaturated fatty acids in seeds from that region. We also constructed a gene co‐expression network to investigate regional variation in network modules. The results of this analysis revealed regional differentiation for eight of 12 modules but no clear relationship with oil biosynthesis. Overall, the differential expression of SAD genes offers a partial explanation for the observed differences in seed oil composition between Texas and Canada, while the expression patterns of other metabolic genes suggest complex regulation of fatty acid production and usage across latitudes.

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Differential transcriptional activity of SAD, FAD2 and FAD3 desaturase genes in developing seeds of linseed contributes to varietal variation in α-linolenic acid content

Cited by 41 publications

References 77 publications

Sequence characterization and in silico structure prediction of fatty acid desaturases in linseed varieties with differential fatty acid composition

Sequence characterization and in silico structure prediction of fatty acid desaturases in linseed varieties with differential fatty acid composition

Transcriptome comparative analysis of two Camellia species reveals lipid metabolism during mature seed natural drying

Transcriptomics of developing wild sunflower seeds from the extreme ends of a latitudinal gradient differing in seed oil composition

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