Differential transformation efficiency of Japonicarice varieties developed in northern China

Ли, Дан; Xu, Hai Jun; Sun, Xiaoxue; Cui, Zhibo; Zhang, Yuan; Bai, Yuguan; Wang, Xiaoxue; Chen, Wenfu

doi:10.1590/1984-70332015v15n3a28

Cited by 8 publications

(4 citation statements)

References 16 publications

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“…In contrast to the in vitro mature-embryo culture of other cereals, as of barley (He and Jia 2008) and rice (Li et al 2015), the rate of somatic embryogenesis of the analyzed wheat species, with the exception of T. diccocum, was low, and they were unable to form more than one green plant per initial explant ( Table 2). The callus regeneration ability of all cultures of AB genome tetraploid wheats, except for T. turgidum, was higher (11.0-28.6%) than that of hexaploid (2.3-8.8%) and diploid wheats (0.7-6.1%).…”

Section: о Alikina Et Almentioning

confidence: 84%

Tissue culture efficiency of wheat species with different genomic formulas

Alikina

Chernobrovkina

Dolgov

et al. 2016

Crop Breed. Appl. Biotechnol.

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Section: о Alikina Et Almentioning

confidence: 84%

Tissue culture efficiency of wheat species with different genomic formulas

Alikina

Chernobrovkina

Dolgov

et al. 2016

Crop Breed. Appl. Biotechnol.

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“…For complementation tests, the CDS of BOS1 fused with a green fluorescent protein (GFP) and the 1,795-bp promoter upstream of ATG were cloned into the pCambia 1300 binary vector with Xba I/ Bam HI and Pst I/ Spe I enzymes, respectively. Then, the BOS1p : BOS1c - GFP plasmid was transformed into a bos1-1 mutant through the agrobacterium ( Agrobacterium tumefaciens )-mediated transformation of rice mature embryos ( Li et al., 2015 ; Zhu et al., 2021 ). In the second and third filial transgenic generations (T 2 and T 3 ), the phenotypes of the complementary transgenic lines harboring BOS1p : BOS1c - GFP construction were observed.…”

Section: Methodsmentioning

confidence: 99%

BOS1 is a basic helix–loop–helix transcription factor involved in regulating panicle development in rice

Zhang

et al. 2023

Front. Plant Sci.

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Panicle development is crucial to increase the grain yield of rice (Oryza sativa). The molecular mechanisms of the control of panicle development in rice remain unclear. In this study, we identified a mutant with abnormal panicles, termed branch one seed 1-1 (bos1-1). The bos1-1 mutant showed pleiotropic defects in panicle development, such as the abortion of lateral spikelets and the decreased number of primary panicle branches and secondary panicle branches. A combined map-based cloning and MutMap approach was used to clone BOS1 gene. The bos1-1 mutation was located in chromosome 1. A T-to-A mutation in BOS1 was identified, which changed the codon from TAC to AAC, resulting in the amino acid change from tyrosine to asparagine. BOS1 gene encoded a grass-specific basic helix–loop–helix transcription factor, which is a novel allele of the previously cloned LAX PANICLE 1 (LAX1) gene. Spatial and temporal expression profile analyses showed that BOS1 was expressed in young panicles and was induced by phytohormones. BOS1 protein was mainly localized in the nucleus. The expression of panicle development-related genes, such as OsPIN2, OsPIN3, APO1, and FZP, was changed by bos1-1 mutation, suggesting that the genes may be the direct or indirect targets of BOS1 to regulate panicle development. The analysis of BOS1 genomic variation, haplotype, and haplotype network showed that BOS1 gene had several genomic variations and haplotypes. These results laid the foundation for us to further dissect the functions of BOS1.

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“…For the complementary test, a 1464 bp MIL1/OsHPL3 CDS fused to Green Fluorescence Protein (GFP) driven by the Cauliflower Mosaic Virus (CaMV) 35S constitutive promoter was cloned into the pCambia1300 binary vector. Then, the resulting 35S:MIL1c-GFP plasmid was introduced into the mil1-1 mutant via the agrobacterium ( Agrobacterium tumefaciens )-mediated transformation of mature rice embryos as described previously [ 39 , 43 ]. In the T 2 and T 3 generations, we observed the phenotypes of complementary transgenic lines containing the 35S:MIL1c/OsHPL3c-GFP construct.…”

Section: Methodsmentioning

confidence: 99%

A Single Amino Acid Substitution in MIL1 Leads to Activation of Programmed Cell Death and Defense Responses in Rice

Yan

Zheng

Sang

et al. 2022

IJMS

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Lesion mimic mutants are an ideal model system for elucidating the molecular mechanisms of programmed cell death and defense responses in rice. In this study, we identified a lesion mimic mutant termed miner infection like 1-1 (mil1-1). The mil1-1 exhibited lesions on the leaves during development, and the chloroplasts of mil1-1 leaves were disrupted. Reactive oxygen species were found to accumulate in mil1-1 leaves. Cell death and DNA fragmentation were observed in mil1-1 leaves, indicating that the cells in the spots of mil1-1 leaves experienced programmed cell death. Most agronomic traits decreased in mil1-1, suggesting that the growth retardation in mil1-1 caused reduced per-plant grain yield. However, the mutation of MIL1 activated the expression of pathogen response genes and enhanced resistance to bacterial blight. The MIL1 gene was cloned using the positional cloning approach. A missense mutation 751 bp downstream of ATG was found in mil1-1. The defects of mil1-1 were able to be rescued by delivering a wild-type MIL1 gene into mil1-1. MIL1 encoded hydroperoxide lyase 3 (OsHPL3), and the expression of OsHPL3 was induced via hormone and abiotic stresses. Our findings provide insights into the roles of MIL1 in regulating programmed cell death, development, yield, and defense responses in rice.

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Differential transformation efficiency of Japonicarice varieties developed in northern China

Abstract: -The production of japonica rice in northern China

Cited by 8 publications

References 16 publications

Tissue culture efficiency of wheat species with different genomic formulas

Tissue culture efficiency of wheat species with different genomic formulas

BOS1 is a basic helix–loop–helix transcription factor involved in regulating panicle development in rice

A Single Amino Acid Substitution in MIL1 Leads to Activation of Programmed Cell Death and Defense Responses in Rice

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