Differential treatment regimen-related effects of cannabinoids on D1 and D2 receptors in adolescent and adult rat brain

Dalton, Victoria S.; Zavitsanou, Katerina

doi:10.1016/j.jchemneu.2010.07.005

Cited by 17 publications

(17 citation statements)

References 71 publications

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“…This suggests a decreased vulnerability to cannabinoids or the presence of other adaptive mechanisms. Previous studies using similar doses and treatment regimens of HU210 as the present study have reported alterations in various brain neurotransmitter systems in adult but not adolescent animals [39][40][41][42]. The reasons why adolescent animals appear to be less sensitive to cannabinoids, as observed in this study and previous studies, remains unclear.…”

Section: Discussionsupporting

confidence: 57%

“…Further, differential responses in the adverse properties of cannabinoids have been reported between adult and adolescent animals [36][37][38] suggesting that adolescent animals experience greater residual impairments and deficits. Similarly, differential responses to cannabinoids between adult and adolescent animals have been found in various neurotransmitter systems, including GABAergic, serotonergic, dopaminergic and cannabinoid receptor systems [39][40][41][42].…”

Section: Introductionmentioning

confidence: 96%

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Testicular Translocator Protein Expression is Differentially Altered by Synthetic Cannabinoid HU210 in Adult and Adolescent Rats

Chan¹

2014

J Addict Res Ther

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Objective: The translocator protein (TSPO) has been implicated in numerous functions including steroid production and regulation of stress and anxiety. Cannabinoids have been shown to reduce plasma testosterone levels and alter anxiety levels. The aim of the present study was to determine whether the synthetic cannabinoid HU210 is able to regulate TSPO expression in several peripheral organs.Methods: HU210 (100 μg/kg) was administered intraperitoneally to both adult and adolescent male ratsfor 14 days. TSPO receptor expression in several organs, including the liver, spleen, kidneys and testes, was quantified by membrane receptor binding using the selective radiolig and, PK11195. In cases where receptor binding data indicated significant cannabinoid-induced differences, further RT-qPCR was carried out to determine the transcriptional regulation of the TSPO gene. Additionally, film-autography was used to identify potential changes in the spatial distribution of the TSPO tissue binding sites. Results:Results indicate that HU210 induces significant reductions in testicular TSPO expression in adult but not adolescent rats. No changes were found in other organs examined. These results are consistent with the previously observed effects of cannabinoids on testosterone production and a presumed role for TSPO in steroidogenesis. Conclusions:Overall, these results suggest that cannabinoids may alter testosterone production by altering the expression of testicular TSPO and that the alteration of TSPO occurs in an age-dependent manner.

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Section: Discussionsupporting

confidence: 57%

Section: Introductionmentioning

confidence: 96%

Testicular Translocator Protein Expression is Differentially Altered by Synthetic Cannabinoid HU210 in Adult and Adolescent Rats

Chan¹

2014

J Addict Res Ther

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“…Results from these studies showed that the acute dose of HU210 only resulted in a decreased CB 1 R density in the CPU and hippocampus; however, a dose-dependent, region-specific downregulation of CB 1 R density was seen in all regions examined after subchronic and chronic treatment [25] . On the other hand, only a significant overall effect of HU210 treatment on D 2 R density was observed; no specific treatment group or individual regions could be identified as a source of variations in D 2 R downregulation [26] .…”

Section: Discussionmentioning

confidence: 80%

“…Our laboratory has previously reported investigations on the effects of HU210 treatment on the CB 1 and D 1 /D 2 receptor densities in the same animals under the same treatment paradigms as in the current investigation [25,26] . Results from these studies showed that the acute dose of HU210 only resulted in a decreased CB 1 R density in the CPU and hippocampus; however, a dose-dependent, region-specific downregulation of CB 1 R density was seen in all regions examined after subchronic and chronic treatment [25] .…”

Section: Discussionmentioning

confidence: 84%

“…To understand the functional linkages of the cannabinoid and dopamine neurotransmitter systems at the receptor expression level, our laboratory previously examined the effects of HU210 on the CB 1 -, D 1 -and D 2 -like receptor densities in the brain of adult and adolescent rats [25,26] . In the present study, we used the same animals and identical experimental paradigms (agonist, doses, treatment durations, animal's age, strain and gender) to investigate the relationship of the agonist dosage regimen and both the D 2 -like and CB 1 agonist-mediated GPCR activations in adult rats.…”

Section: Introductionmentioning

confidence: 99%

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Differential Treatment Regimen-Related Effects of HU210 on CB<sub>1</sub> and D<sub>2</sub>-Like Receptor Functionality in the Rat Basal Ganglia

Nguyen

Wang²,

Verdurand³

et al. 2012

Pharmacology

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Background/Aims: Functional linkages between the cannabinoid CB₁ and the dopaminergic systems have been reported although the observations and the mechanisms hypothesizing their interactions at the G protein-coupled receptor (GPCR) functionality level are conflicting. Methods: Administration of a potent cannabinoid agonist, HU210, at various doses (25–100 µg/kg) and treatment regimens (1- to 14-day treatment) in rats was carried out to investigate the effect of HU210 treatment on the CB₁ and D₂-like agonist-mediated GPCR activation. Results: The desensitizations (reduced coupling) of both D₂ agonist- and CB₁ agonist-mediated GPCR activation was found to be treatment duration dependent and region specific, suggesting implication of receptor tolerance and adaptation due to the cannabinoid treatment. The effect of HU210 on the CB₁ agonist-mediated GPCR desensitization in all treatment groups was not dose dependent. Conclusions: The desensitization of D₂-like receptors found after a cannabinoid treatment in this study strengthens the evidence that the two neurotransmitter systems interact at the intercellular level; this interaction might occur via multiple mechanisms, which also vary according to region.

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Synthetic Cannabinoids and Neurodevelopment

Silva

Carmo

Carvalho

2022

Handbook of Substance Misuse and Addictions

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Differential treatment regimen-related effects of cannabinoids on D1 and D2 receptors in adolescent and adult rat brain

Cited by 17 publications

References 71 publications

Testicular Translocator Protein Expression is Differentially Altered by Synthetic Cannabinoid HU210 in Adult and Adolescent Rats

Testicular Translocator Protein Expression is Differentially Altered by Synthetic Cannabinoid HU210 in Adult and Adolescent Rats

Differential Treatment Regimen-Related Effects of HU210 on CB<sub>1</sub> and D<sub>2</sub>-Like Receptor Functionality in the Rat Basal Ganglia

Synthetic Cannabinoids and Neurodevelopment

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