Differential turnover of the photosystem II reaction centre D1 protein in mesophyll and bundle sheath chloroplasts of maize

Pokorska, Berenika; Zienkiewicz, Maksymilian; Powikrowska, Marta; Drożak, Anna; Romanowska, Elżbieta

doi:10.1016/j.bbabio.2009.05.002

Cited by 40 publications

(37 citation statements)

References 62 publications

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“…Furthermore, the accessibility and distribution of effectors, such as inhibitors, is better and more homogeneous in protoplasts. For example, inhibition of plastidial protein synthesis requires several hours of incubation in a lincomycin solution and/or vacuum infiltration (30,32), which can be avoided by using protoplasts (Materials and Methods). Protoplasts also can be used directly for CLSM studies, allowing for fast and easy isolation of thylakoid membranes and their subfragments.…”

Section: Resultsmentioning

confidence: 99%

Architectural switch in plant photosynthetic membranes induced by light stress

Herbstová

Tietz

Kinzel

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

143

113

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Unavoidable side reactions of photosynthetic energy conversion can damage the water-splitting photosystem II (PSII) holocomplex embedded in the thylakoid membrane system inside chloroplasts. Plant survival is crucially dependent on an efficient molecular repair of damaged PSII realized by a multistep repair cycle. The PSII repair cycle requires a brisk lateral protein traffic between stacked grana thylakoids and unstacked stroma lamellae that is challenged by the tight stacking and low protein mobility in grana. We demonstrated that high light stress induced two main structural changes that work synergistically to improve the accessibility between damaged PSII in grana and its repair machinery in stroma lamellae: lateral shrinkage of grana diameter and increased protein mobility in grana thylakoids. It follows that high light stress triggers an architectural switch of the thylakoid network that is advantageous for swift protein repair. Studies of the thylakoid kinase mutant stn8 and the double mutant stn7/8 demonstrate the central role of protein phosphorylation for the structural alterations. These findings are based on the elaboration of mathematical tools for analyzing confocal laser-scanning microscopic images to study changes in the sophisticated thylakoid architecture in intact protoplasts.confocal microscopy | macromolecular crowding | photosynthesis P hotosynthetic transformation of sunlight into metabolic energy equivalents is a dangerous venture, because toxic side products of the primary photochemical processes can lead to uncontrolled damage. Harmful photosynthetic side reactions cannot be avoided completely and become a serious problem under stress (e.g., high light stress). The main target of photoinhibition (PI) is the D1 subunit of the water-splitting photosystem II (PSII) (1, 2). The D1 subunit is buried in the massive (1,400 kDa) PSII holocomplex that is organized as a dimer and binds between two and four trimeric light-harvesting complex IIs (LHCIIs) (3, 4). Estimates predict that without repair of damaged PSII, the efficiency for photosynthetic energy conversion would drop below 5% (5). Consequently, plants would not survive in a highly dynamic and competitive natural environment. Plants address this challenge through the evolutionary invention of one of the fastest and most efficient molecular repair mechanisms in nature, the PSII repair cycle (5-7).The PSII repair cycle consist of a series of events, including phosphorylation/ dephosphorylation of PSII subunits, holocomplex disassembly/reassembly, and D1 degradation/de novo synthesis (8-10). All of these reactions are harbored in or at the thylakoid membrane system inside the chloroplast. The complex folding of the thylakoid membrane leads to a structural differentiation into stacked grana regions interconnected by unstacked stroma lamellae (11)(12)(13). This structural heterogeneity is accompanied by, and partly driven by, differential protein distributions. PSII and LHCII are concentrated in grana stacks, photosystem I (PSI) and the ATPas...

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Section: Resultsmentioning

confidence: 99%

Architectural switch in plant photosynthetic membranes induced by light stress

Herbstová

Tietz

Kinzel

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

143

113

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“…2, but the values of A above 1,000 lmol m -2 s -1 of PPFD do not increase to a sufficient extent to consume the increased reducing equivalent and ATP produced at high PPFD and thus to prevent ROS production and accelerate the repair of PS II. Therefore, PPFD above 1,000 lmol m -2 s -1 can cause photoinhibition, even in a C 4 plant like corn (Pokorska et al 2009), due to the reduction in F v /F m while under 1,000 lmol m -2 s -1 even for only three hours exposure ( Fig. 3), with slow recovery following dark adaptation.…”

Section: Introductionmentioning

confidence: 94%

“…carbohydrates, is required by most life on this planet to produce the main cellular ''fuel'' ATP by photosynthesis. However, an excess of light in the presence of O 2 (a strong oxidant) can cause damage, particularly to photosystem II (PS II) in the thylakoid membranes of chloroplasts in plants (Pokorska et al 2009). This phenomenon is called photoinhibition of the photosynthetic apparatus and can lead to a reversible or irreversible photooxidative damage of organic compounds of the photosynthesis apparatus (Osmond et al 1980), especially proteins that organize chlorophylls in the light-harvesting complex II (LHC II) associated with PS II, but also in the LHC I associated to PS I (Nishiyama et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Photoinhibition in a C4 plant, Zea mays L.: a minireview

Pimentel

2014

Theor. Exp. Plant Physiol.

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Light and oxygen are both essential to life but can also cause some damage to plant photosynthesis and productivity, particularly excess light in the presence of oxygen. This phenomenon is called photoinhibition, i.e. the photooxidative damage of photosystems due to excess light associated or not with abiotic stresses. To tolerate photoinhibition, the plant needs to be able to avoid or tolerate excess light or to repair the photodamage by maintaining the de novo D1 protein synthesis that is essential for PS II complex activity. When the electron transport of the photosystems is diminished because the use of final electron acceptors, ferredoxin or NADPH 2 , via the Calvin cycle is reduced, electrons from water photolysis are captured by O 2 forming ROS, which inhibits protein synthesis, even in C 4 plants. To tolerate excess light or repair photodamage under photoinhibitory conditions, several mechanisms exist to reduce ROS and/or maintain ATP production and use for protein synthesis and growth: the scavenging systems (reducing ROS activity), such as the xanthophyll (thermal dissipation of excitation energy) and ascorbate cycle (associated with the Mehler reaction, in the waterwater cycle) or the activity of antioxidants compounds, such as carotenoids; the maintenance of cyclic electron flow (only ATP production); and the photorespiration cycle (consuming reducing equivalents and ATP). Photoinhibition is a phenomenon that occurs in C 3 and C 4 plants, such as corn, under PPFD above 1,000 lmol m -2 s -1 , and photooxidative damage seems to be more accentuated in mesophyll chloroplasts than in bundle sheath agranal chloroplasts.

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“…Ce postulat peut être renforcé par la constance de Fo qui écarte la possibilité d'endommagement des centres réactionnels du PSII, entre autre, les protéines D1 et D2. Pour les feuilles intactes, lorsque ces protéines sont détruites, la récupération de l'activité photochimique est conditionnée par leur néo-synthèse (Takahashi et al, 2008;Pokorska et al, 2009).…”

Section: Discussionunclassified

Effet de l’interaction lumiere-salinite sur l’activite du photosysteme ii des feuilles excisees de maïs

Maatallah¹,

Hajlaoui²,

Boughalleb³

et al. 2015

Afr. Crop Sci. J.

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The decline in growth observed in many plants, subjected to salt stress and exposed to sunlight conditions, is often associated with a decrease in their photosynthetic activity. No clear mechanisms of the inhibited photosynthesis have emerged since photosystem II (PS II) is considered to play a key role in the response of leaf photosynthesis to environmental perturbations. The combination of light and salt stress appears to have synergistic effects on the photochemical activity of PSII driving, and to photoinhibition. The objective of this study was to evaluate the reversible effect of salinity and light interaction on maximal quantum efficiency of photosystem II (Fv/Fm.). In this experiment, detached leaves of two forage maize (Zea mays L.) varieties, Aristo and Arper were placed during 6 hours in solutions of different concentrations of NaCl (0, 100, 200 and 300 mM) and subjected to light (1000 µmol m -2 s -1 ) or obscurity. Then, their contents of sodium were determined. In order to verify the photo-inhibition reversibility, other leaves which were incubated in a solution of 300 Mm NaCl, during 4 hours were transferred in distilled water and also subjected to light or to obscurity. Results indicate that leaves which had been put to absorb NaCl in obscurity showed no change in maximal efficiency of PSII (Fv/Fm). Nevertheless, light treatment associated with salinity generates a photo-inhibition of PSII manifested by a significant decrease in maximal efficiency of PSII. This photo-inhibition, due to an excessive accumulation of sodium in leaves, is reversible. It is quite sufficient to eliminate only one factor of the association light-salinity for the PSII activity resume.Key Words: Light stress, sodium chloride, Zea mays RESUME La diminution de la croissance vegetative observée chez nombreuses plantes, soumises au stress salin et exposées à des conditions naturelles d'ensoleillement, est souvent associée à une baisse de leur activité photosynthétique. Cependant les mécanismes de l'inhibition photosynthétique sont encore peu étudiés. Le photosystème II (PS II) est considéré un des facteurs clé dans la réponse de la photosynthèse des feuilles aux stress environnementaux. L'association de la lumière et du stress salin parait avoir des effets synergétiques sur l'activité photochimique du PSII conduisant, ainsi à la photoinhibition. L'objectif de cette etude est de diagnostiquer l'effet de l'interaction lumière-salinité sur l'activité photochimique du photosystème II au cours de la photosynthèse. Le materiel vegetal est constitué de deux variétés de maïs (Zea mays L.) fourrager: Aristo et Arper. Des feuilles détachées de plantes cultivées sur milieu témoin (eau distilée), sont incubées pendant 6 heures dans des solutions salines à différentes concentrations (0, 100, 200 et 300 mM NaCl), soit en absence ou en présence de lumière (1000 µmol m -2 s -1). Puis, leurs teneurs en sodium ont été déterminées. Pour vérifiers'il y'aura récupération de leur activité photochimique, d'autres feuilles qui ont été mises à...

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Differential turnover of the photosystem II reaction centre D1 protein in mesophyll and bundle sheath chloroplasts of maize

Cited by 40 publications

References 62 publications

Architectural switch in plant photosynthetic membranes induced by light stress

Architectural switch in plant photosynthetic membranes induced by light stress

Photoinhibition in a C4 plant, Zea mays L.: a minireview

Effet de l’interaction lumiere-salinite sur l’activite du photosysteme ii des feuilles excisees de maïs

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