Differential Wnt11 Expression Related to Wnt5a in High- and Low-grade Serous Ovarian Cancer: Implications for Migration, Adhesion and Survival

Jannesari-Ladani, F; Hossein, Ghamartaj; Izadi-Mood, Narges

doi:10.7314/apjcp.2014.15.3.1489

Cited by 7 publications

(4 citation statements)

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“…Several studies have demonstrated that alteration of PI3K/AKT/PTEN and Wnt/ β-catenin was found in many cancers and these pathways may serve as the potential targets for cancer treatment (Gong et al, 2012;Pandurangan, 2013;Jannesari-Ladani et al, 2014;Tan et al, 2014). This is the first report of the alteration of the protein components comprising the PI3K/ AKT/PTEN and Wnt/β-catenin pathway in a hamster model of Ov-induced CCA carcinogenesis.…”

Section: Discussionmentioning

confidence: 71%

Opisthorchis viverrini Infection Activates the PI3K/AKT/PTEN and Wnt/β-catenin Signaling Pathways in a Cholangiocarcinogenesis Model

Yothaisong¹,

Thanee²,

Namwat³

et al. 2015

Asian Pacific Journal of Cancer Prevention

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Opisthorchis viverrini (Ov) infection is the major etiological factor for cholangiocarcinoma (CCA), especially in northeast Thailand. We have previously reported significant involvement of PI3K/AKT/PTEN and Wnt/β-catenin in human CCA tissues. The present study, therefore, examined the expression and activation of PI3K/ AKT/PTEN and Wnt/β-catenin signaling components during Ov-induced cholangiocarcinogenesis in a hamster animal model. Hamsters were divided into two groups; non-treated and Ov plus NDMA treated. The results of immunohistochemical staining showed an upregulation of PI3K/AKT signaling as determined by elevated expression of the p85α-regulatory and p110α-catalytic subunits of PI3K as well as increased expression and activation of AKT during cholangiocarcinogenesis. Interestingly, the staining intensity of activated AKT (p-AKT) increased in the apical regions of the bile ducts and strong staining was detected where the liver fluke resides. Moreover, PTEN, a negative regulator of PI3K/AKT, was suppressed by decreased expression and increased phosphorylation during cholangiocarcinogenesis. We also detected upregulation of Wnt/β-catenin signaling as determined by increased positive staining of Wnt3, Wnt3a, Wnt5a, Wnt7b and β-catenin, corresponded with the period of cholangiocarcinogenesis. Furthermore, nuclear staining of β-catenin was observed in CCA tissues. Our results suggest the liver fluke infection causes chronic inflammatory conditions which lead to upregulation of the PI3K/AKT and Wnt/β-catenin signaling pathways which may drive CCA carcinogenesis. These results provide useful information for drug development, prevention and treatment of CCA.

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Section: Discussionmentioning

confidence: 71%

Opisthorchis viverrini Infection Activates the PI3K/AKT/PTEN and Wnt/β-catenin Signaling Pathways in a Cholangiocarcinogenesis Model

Yothaisong¹,

Thanee²,

Namwat³

et al. 2015

Asian Pacific Journal of Cancer Prevention

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“…One such promising functional biomarker is Wnt-11, a member of a family of secreted proteins that regulate tissue growth and morphogenesis during development. It has previously been reported that Wnt-11 also plays a significant pathophysiological role in major carcinomas, e.g., cancers of prostate, cervical, ovarian and colon and controls neuroendocrine differentiation [16,17,18,19]. Wnt-11 is downstream of TGFβ—shown previously to be one of the triggers of epithelial-mesenchymal transition (EMT) and chemoresistance [20,21,22].…”

Section: Introductionmentioning

confidence: 99%

Wnt-11 Expression Promotes Invasiveness and Correlates with Survival in Human Pancreatic Ductal Adeno Carcinoma

Dart

Arısan

Owen

et al. 2019

Genes

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Pancreatic ductal adenocarcinoma (PDAC) is one of the deadliest forms of cancer, proving difficult to manage clinically. Wnt-11, a developmentally regulated gene producing a secreted protein, has been associated with various carcinomas but has not previously been studied in PDAC. The present study aimed to elucidate these aspects first in vitro and then in a clinical setting in vivo. Molecular analyses of Wnt-11 expression as well as other biomarkers involved qRT-PCR, RNA-seq and siRNA. Proliferation was measured by MTT; invasiveness was quantified by Boyden chamber (Matrigel) assay. Wnt-11 mRNA was present in three different human PDAC cell lines. Wnt-11 loss affected epithelial-mesenchymal transition and expression of neuronal and stemness biomarkers associated with metastasis. Indeed, silencing Wnt-11 in Panc-1 cells significantly inhibited their Matrigel invasiveness without affecting their proliferative activity. Consistently with the in vitro data, human biopsies of PDAC showed significantly higher Wnt-11 mRNA levels compared with matched adjacent tissues. Expression was significantly upregulated during PDAC progression (TNM stage I to II) and maintained (TNM stages III and IV). Wnt-11 is expressed in PDAC in vitro and in vivo and plays a significant role in the pathophysiology of the disease; this evidence leads to the conclusion that Wnt-11 could serve as a novel, functional biomarker PDAC.

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“…Genes SIAH2 [47], CDH5 [48] and HS3ST2 [49] were reported to be associated with breast cancer. Genes WNT11 [50] and THPO [51] were reported to be associated with ovarian cancer and colorectal cancer, respectively. Genes C1orf114 [39], CA3 [52] and KRT4 [53] were reported to be associated with prostate cancer, hepatocellular carcinoma and esophageal squamous cell carcinoma, respectively.…”

Section: Resultsmentioning

confidence: 99%

Incorporating genetic networks into case-control association studies with high-dimensional DNA methylation data

Kim

Sun

2019

BMC Bioinformatics

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BackgroundIn human genetic association studies with high-dimensional gene expression data, it has been well known that statistical selection methods utilizing prior biological network knowledge such as genetic pathways and signaling pathways can outperform other methods that ignore genetic network structures in terms of true positive selection. In recent epigenetic research on case-control association studies, relatively many statistical methods have been proposed to identify cancer-related CpG sites and their corresponding genes from high-dimensional DNA methylation array data. However, most of existing methods are not designed to utilize genetic network information although methylation levels between linked genes in the genetic networks tend to be highly correlated with each other.ResultsWe propose new approach that combines data dimension reduction techniques with network-based regularization to identify outcome-related genes for analysis of high-dimensional DNA methylation data. In simulation studies, we demonstrated that the proposed approach overwhelms other statistical methods that do not utilize genetic network information in terms of true positive selection. We also applied it to the 450K DNA methylation array data of the four breast invasive carcinoma cancer subtypes from The Cancer Genome Atlas (TCGA) project.ConclusionsThe proposed variable selection approach can utilize prior biological network information for analysis of high-dimensional DNA methylation array data. It first captures gene level signals from multiple CpG sites using data a dimension reduction technique and then performs network-based regularization based on biological network graph information. It can select potentially cancer-related genes and genetic pathways that were missed by the existing methods.Electronic supplementary materialThe online version of this article (10.1186/s12859-019-3040-x) contains supplementary material, which is available to authorized users.

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Differential Wnt11 Expression Related to Wnt5a in High- and Low-grade Serous Ovarian Cancer: Implications for Migration, Adhesion and Survival

Cited by 7 publications

References 37 publications

Opisthorchis viverrini Infection Activates the PI3K/AKT/PTEN and Wnt/β-catenin Signaling Pathways in a Cholangiocarcinogenesis Model

Opisthorchis viverrini Infection Activates the PI3K/AKT/PTEN and Wnt/β-catenin Signaling Pathways in a Cholangiocarcinogenesis Model

Wnt-11 Expression Promotes Invasiveness and Correlates with Survival in Human Pancreatic Ductal Adeno Carcinoma

Incorporating genetic networks into case-control association studies with high-dimensional DNA methylation data

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