Differentially expressed genes following persistent infection with infectious pancreatic necrosis virus in vitro and in vivo

Marjara, Inderjit S.; Thu, Beate; Evensen, Øystein

doi:10.1016/j.fsi.2010.02.001

Cited by 27 publications

(9 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…5A), indicating EsNACA is possibly involved in early response to viral infections. In consistent with this hypothesis, previous study has revealed that NACA is a differentially expressed gene in Chinook salmon embryonic cells by infectious pancreatic necrosis virus infections [25] and in P. monodon hemocytes following infection with yellow head virus [26]. In addition, LPS challenge also resulted in a significant induction (16.5-fold increase than untreated controls) of EsNACA expression at 24 h (Fig.…”

Section: Esnaca Expression In Response To Immune Challengessupporting

confidence: 84%

Identification and characterization of nascent polypeptide-associated complex alpha from Chinese mitten crab (Eriocheir sinensis): A novel stress and immune response gene in crustaceans

Peng

Chen

et al. 2016

Fish & Shellfish Immunology

View full text Add to dashboard Cite

Section: Esnaca Expression In Response To Immune Challengessupporting

confidence: 84%

Identification and characterization of nascent polypeptide-associated complex alpha from Chinese mitten crab (Eriocheir sinensis): A novel stress and immune response gene in crustaceans

Peng

Chen

et al. 2016

Fish & Shellfish Immunology

View full text Add to dashboard Cite

“…Cell lines persistent infection with adenovirus, papillomavirus, polyomavirus, reovirus, birnavirus or nodavirus have been reported (Ahmed, Chakraborty, Graham, Ramig, & Fields, ; Ahmed & Graham, ; Bedell et al, ; Chi et al, ; Delli Bovi, De Simone, Giordano, & Amati, ; Marjara, Thu, & Evensen, ; Markel et al, ; Wen, ). These persistent infections are possibly associated with host cell status and virus type.…”

Section: Discussionmentioning

confidence: 99%

Persistent development of adomavirus and aquareovirus in a novel cell line from marbled eel with petechial skin haemorrhage

Wen

2019

Journal of Fish Diseases

View full text Add to dashboard Cite

In Taiwan, a petechial haemorrhage disease associated with mortality has affected marbled eels (Anguilla marmorata). The eels were revealed to be infected with adomavirus (MEAdoV, previously recognized as a polyoma‐like virus). In this study, cell line DMEPF‐5 was established from the pectoral fin of a diseased eel. DMEPF‐5 was passaged >70 times and thoroughly proliferated in L‐15 medium containing 2%–15% foetal bovine serum at 20–30°C. Transcripts of neural cell adhesion molecule 1 and nestin genes, and nucleic acids of MEAdoV and a novel reovirus (MERV) in the cells were demonstrated by reverse transcription‐polymerase chain reaction analysis. Phylogenetic analysis revealed that the AdoV LO8 proteins mostly relate to adenovirus adenain, whereas MERV is close to American grass carp reovirus in Aquareovirus G, based on a partial VP2 nucleotide sequence. DMEPF‐5 cells are susceptible to additional viral infection. Taken together, the marbled eels with the haemorrhagic disease have coinfection with MEAdoV and MERV, and the pathogenic role of MEAdoV and MERV warrants research. DMEPF‐5 has gene expression associated with mesenchymal stem and progenitor cells and is the first cell line persistently infected with adomavirus and aquareovirus. DMEPF‐5 can facilitate studies of such viruses and haemorrhagic disease.

show abstract

“…While the general understanding would be that naked viruses release their progeny through cell lysis (38), our findings support virus release occurring without CPE being found by visual inspection of the cell cultures. This observation is not unique, and release of another fish pathogenic virus, infectious pancreatic necrosis virus, into cell culture supernatants at high copy numbers without obvious CPE has been observed (19,22). Further, release of rotavirus from polarized epithelial cells in vitro has also been observed to occur without cell lysis (17).…”

mentioning

confidence: 99%

Cardiomyopathy Syndrome of Atlantic Salmon (Salmo salar L.) Is Caused by a Double-Stranded RNA Virus of the Totiviridae Family

Haugland

Mikalsen

Nilsen

et al. 2011

J Virol

Self Cite

171

209

View full text Add to dashboard Cite

Cardiomyopathy syndrome (CMS) of farmed and wild Atlantic salmon (Salmo salar L.) is a disease of yet unknown etiology characterized by a necrotizing myocarditis involving the atrium and the spongious part of the heart ventricle. Here, we report the identification of a double-stranded RNA virus likely belonging to the family Totiviridae as the causative agent of the disease. The proposed name of the virus is piscine myocarditis virus (PMCV). On the basis of the RNA-dependent RNA polymerase (RdRp) sequence, PMCV grouped with Giardia lamblia virus and infectious myonecrosis virus of penaeid shrimp. The genome size of PMCV is 6,688 bp, with three open reading frames (ORFs). ORF1 likely encodes the major capsid protein, while ORF2 encodes the RdRp, possibly expressed as a fusion protein with the ORF1 product. ORF3 seems to be translated as a separate protein not described for any previous members of the family Totiviridae. Following experimental challenge with cell culture-grown virus, histopathological changes are observed in heart tissue by 6 weeks postchallenge (p.c.), with peak severity by 9 weeks p.c. Viral genome levels detected by real-time reverse transcription (RT)-PCR peak earlier at 6 to 7 weeks p.c. The virus genome is detected by in situ hybridization in degenerate cardiomyocytes from clinical cases of CMS. Virus genome levels in the hearts from clinical field cases correlate well with the severity of histopathological changes in heart tissue. The identification of the causative agent for CMS is important for improved disease surveillance and disease control and will serve as a basis for vaccine development against the disease.

show abstract

Differentially expressed genes following persistent infection with infectious pancreatic necrosis virus in vitro and in vivo

Cited by 27 publications

References 39 publications

Identification and characterization of nascent polypeptide-associated complex alpha from Chinese mitten crab (Eriocheir sinensis): A novel stress and immune response gene in crustaceans

Identification and characterization of nascent polypeptide-associated complex alpha from Chinese mitten crab (Eriocheir sinensis): A novel stress and immune response gene in crustaceans

Persistent development of adomavirus and aquareovirus in a novel cell line from marbled eel with petechial skin haemorrhage

Cardiomyopathy Syndrome of Atlantic Salmon (Salmo salar L.) Is Caused by a Double-Stranded RNA Virus of the Totiviridae Family

Contact Info

Product

Resources

About