Differentially expressed genes related to plant height and yield in two alfalfa cultivars based on RNA-seq

Qi, Jiangjiao; Yu, Xiaoqin; Xu, Qun; Zhang, Fanfan; Ma, Chunhui

doi:10.7717/peerj.14096

Cited by 4 publications

(2 citation statements)

References 58 publications

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“…The TK in the soil was determined using sodium hydroxide melting and flame photometry. The detection and calculation of plant agronomic traits (PHT, FW, ILH, SD, and NOB) were based on a report in the literature 58 .…”

Section: Methodsmentioning

confidence: 99%

The effect of alfalfa cultivation on improving physicochemical properties soil microorganisms community structure of grey desert soil

Qi,

Fu,

Wang

et al. 2023

Sci Rep

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Planting alfalfa in grey desert soil can have significant effects on soil nutrient levels, microbial communities, and overall soil improvement. High-throughput sequencing technology was used to explore the relationship between the rhizosphere microbial community structure of grey desert soil planted with different alfalfa varieties (Aohan, WL525HQ, Knight2, Kangsai, Victoria, and WL712), alfalfa characteristics and rhizosphere soil physicochemical properties. Alfalfa planting increased the nitrogen and organic matter in the grey desert soil, and the effects in Victoria, Kangsai, and Aohan were relatively better than those in the unplanted areas and other alfalfa areas. The Chao1 and Shannon indexes showed that the diversity and relative abundance of bacteria and fungi in Kangsai were significantly higher than those in the unplanted areas and other alfalfa areas. Redundancy analysis showed that available nitrogen and phosphorus, as well as fresh weight, significantly affected the changes in fungal and bacterial communities. Variance partitioning analysis showed that soil and alfalfa growth characteristics explained 50.04% and 51.58% of the structural changes in the bacteria and fungi, respectively. Therefore, planting alfalfa changed the community structure of bacteria and fungi, as well as the content of soil nutrients, and different varieties of alfalfa had different effects on soil improvement.

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Section: Methodsmentioning

confidence: 99%

The effect of alfalfa cultivation on improving physicochemical properties soil microorganisms community structure of grey desert soil

Qi,

Fu,

Wang

et al. 2023

Sci Rep

Self Cite

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show abstract

“…Three plants of uniform plant size and neat flowering were randomly selected from each pot, and six replicates of each species were used to determine the phenotypic trait indexes of the plants [24]. The height from the base of the forage to the tip of the leaf or the top of the inflorescence was measured as plant height (PH) using a tape measure.…”

Section: Phenotypic Trait Indicatorsmentioning

confidence: 99%

Analysis of Phenotypic and Physiological Characteristics of Plant Height Difference in Alfalfa

Fang

Shi

et al. 2023

Agronomy

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Cultivating new alfalfa (Medicago sativa L.) varieties with high yield and quality is of great significance for improving alfalfa yield and promoting the development of the grass and livestock industry. Plant height is an important indicator of alfalfa yield and is closely related to photosynthetic capacity, harvest index and yield. However, the underlying cause of the variation in height among alfalfa plants is not clear. In this paper, we measured the phenotypic traits, photosynthetic physiology and endogenous hormone content of tall- and short-stalked alfalfa materials and analyzed the important external and internal factors that caused the difference in plant height of alfalfa. We found that the phenotypic traits of tall- and short-stalked alfalfa materials showed significant differences, and dwarf alfalfa showed significant shortening of the main stem internode length. There were also some differences in light and physiological indicators and endogenous hormone contents between tall- and short-stalked alfalfa materials. Through correlation analysis, we found that the phenotypic traits and physiological indicators significantly correlated with alfalfa plant height were the number of internodes, stem diameter, average internode length, leaf–stem ratio, leaf area, Pn (net photosynthetic rate), Tr (transpiration rate), upper leaf SP (soluble protein), Suc (sucrose) content, middle stem Sta (starch) content, middle stem ZT (zeatin) and IAA (indole-3-acetic acid). Further analysis showed that Tr, IAA and LA played a direct role in plant height, with Tr contributing the most to plant height, followed by IAA. Finally, we found that the starch content of the middle stem had a significant impact on plant height through principal component analysis. These results provide new insights into the formation and genetic improvement of plant height traits in leguminous forages such as alfalfa.

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Xylem cell size regulation is a key adaptive response to water deficit in Eucalyptus grandis

Keret,

Drew,

Hills

2024

Tree Physiology

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Future climatic scenarios forecast increasingly frequent droughts that will pose substantial consequences on tree mortality. In light of this, drought-tolerant eucalypts have been propagated, however, the severity of these conditions will invoke adaptive responses, impacting the commercially valuable wood properties. To determine what mechanisms govern the wood anatomical adaptive response, highly controlled drought experiments were conducted in E. grandis, with the tree physiology and transcriptome closely monitored. In response to water deficit, E. grandis displays an isohydric stomatal response to conserve water and enable stem growth to continue, albeit at a reduced rate. Maintaining gaseous exchange is likely a critical short-term response that drives the formation of hydraulically-safer xylem. For instance, the development of significantly smaller fibers and vessels was found to increase cellular density, thereby promoting drought tolerance through improved functional redundancy, as well as implosion and cavitation resistance. The transcriptome was explored to identify the molecular mechanisms responsible for controlling xylem cell size during prolonged water deficit. Downregulation of genes associated with cell wall remodelling and the biosynthesis of cellulose, hemicellulose, and pectin appeared to coincide with a reduction in cellular enlargement during drought. Furthermore, transcript levels of NAC and MYB transcription factors, vital for cell wall component biosynthesis, were reduced, while those linked to lignification increased. The upregulation of EgCAD and various peroxidases under water deficit did not correlate with an increased lignin composition. However, with the elevated cellular density, a higher lignin content per xylem cross-sectional area was observed, potentially enhancing hydraulic safety. These results support the requirement for higher density, drought-adapted wood as a long-term adaptive response in E. grandis, which is largely influenced by the isohydric stomatal response coupled with cellular expansion-related molecular processes.

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Differentially expressed genes related to plant height and yield in two alfalfa cultivars based on RNA-seq

Cited by 4 publications

References 58 publications

The effect of alfalfa cultivation on improving physicochemical properties soil microorganisms community structure of grey desert soil

The effect of alfalfa cultivation on improving physicochemical properties soil microorganisms community structure of grey desert soil

Analysis of Phenotypic and Physiological Characteristics of Plant Height Difference in Alfalfa

Xylem cell size regulation is a key adaptive response to water deficit in Eucalyptus grandis

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