Differentially expressed lncRNAs, miRNAs and mRNAs with associated ceRNA networks in a mouse model of myocardial ischemia/reperfusion injury

Abstract

Non-coding RNAs, including long non-coding RNAs (lncRNAs) and microRNAs (miRNAs/miRs), have significant regulatory effects on a number of biological processes in myocardial ischemia/reperfusion (I/R) injury, including cell differentiation, proliferation and apoptosis. In the present study, the expression levels of lncRNAs, miRNAs and mRNAs were evaluated in a mouse model of myocardial I/R injury. The potential functions of these differentially expressed genes were then analyzed via Gene Ontology and pathway analyses. Additionally, the interactions between lncRNA-miRNA-mRNA were predicted by constructing a competing endogenous RNA regulatory network. It was found that 14,366 lncRNAs, 151 miRNAs and 9,377 mRNAs were differentially expressed in mice hearts after I/R compared with the Sham group (fold change >2; P<0.05). The results indicated that these differentially expressed genes were involved in multiple molecular functions, including ‘guanosine diphosphate binding’, ‘RNA polymerase II carboxy-terminal domain kinase activity’, ‘TATA-binding protein-class protein binding’, ‘nicotinamide adenine dinucleotide binding’ and ‘protein phosphatase type 2A regulator activity’. The interactions between lncRNA-miRNA-mRNA, including five lncRNAs, 38 miRNAs and 196 mRNAs, were predicted, specifically Gm12040-mmu-miR-125a-5p-decapping mRNA 1B, Rpl7l1-ps1-mmu-miR-124-3p-G protein-coupled receptor 146, Gm11407-mmu-miR-190a-5p-homeobox and leucine zipper encoding (HOMEZ), 1600029O15Rik-mmu-miR-132-3p-HOMEZ and AK155692-mmu-miR-1224-3p-activating transcription factor 6β. Collectively, these findings provided novel insights for future research on lncRNAs, miRNAs and mRNAs in myocardial I/R injury.

Long Non-Coding RNA SOX2 Overlapping Transcript Aggravates H9c2 Cell Injury via the miR-215-5p/ZEB2 Axis and Promotes Ischemic Heart Failure in a Rat Model

“…Long noncoding RNA can act as a molecular sponge for miRNA to release the downstream target mRNA (Zhou et al 2018;Zhang et al 2020;Zou et al 2020). We anticipated that SOX2-OT regulated H9c2 cell injury by this pattern.…”

Regulation of Oxidative Stress by Long Non-coding RNAs in Central Nervous System Disorders

“…Regulating lncRNAs expression has neuroprotective effects in AIS cells and animal models. In addition, most of these lncRNAs regulate the expression of their downstream mRNAs by sponging their target miRNAs, which regulates oxidative stress ( Zhang et al, 2020c ). These recent studies provide a new mechanism for lncRNAs to participate in AIS pathogenesis and suggest potential novel therapeutic strategies for AIS.…”

Bioinformatics-Based Analysis of the lncRNA-miRNA-mRNA Network and TF Regulatory Network to Explore the Regulation Mechanism in Spinal Cord Ischemia/Reperfusion Injury

“…Hence, the inhibitory effect of miRNA on mRNA might be canceled when miRNAs is combined with MRE on lncRNA. Recently, accumulating studies have demonstrated that the ceRNA network has become increasingly important in the occurrence and progression of diverse diseases, including cancers, Parkinson’s disease, myocardial ischemia/reperfusion injury, and cerebral infarction ( Zou et al, 2019 ; Qi et al, 2020 ; Zhang R. et al, 2020 ; Zhang X. et al, 2020 ). Nonetheless, few studies of ceRNA network in SCII have been reported ( Liu et al, 2018 ; Qiao et al, 2018 ).…”