Differentially expressed miRNAs in anthers may contribute to the fertility of a novel Brassica napus genic male sterile line CN12A

Dong, Yun; Wang, Yi; Jin, F.F.; Xing, Lijuan; Fang, Yan; Zheng-ying, Zhang; Zou, Junjie; Wang, Lei; Xu, Miaoyun

doi:10.1016/s2095-3119(19)62780-2

Cited by 11 publications

(7 citation statements)

References 37 publications

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“…Zhang et al performed small RNA and transcriptome sequencing of cytoplasmic male sterile cotton buds and found that gra-miR7505b could precisely cut 643 and 748 nt sites to regulate the pentatricopeptide repeat (PPR; Gh_D05G3392) gene, leading to the upregulation of the PPR gene and the restoration of male fertility [ 32 ]. Other studies have also found that miRNAs regulate gene expression, mostly through the cutting of target genes to affect anther and fertility development [ 33 , 34 ]. The miRNAs identified in cotton anther development after high-temperature stress were divided into seven categories according to miRNA families, and Chen et al found that the miRNA responses to high-temperature stress were specific to anther changes at different developmental stages [ 35 ].…”

Section: Introductionmentioning

confidence: 99%

Integrated Analysis of Small RNA, Transcriptome, and Degradome Sequencing Reveals the MiR156, MiR5488 and MiR399 Are Involved in the Regulation of Male Sterility in PTGMS Rice

Sun

Xiong

Wang

et al. 2021

IJMS

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A photoperiod- and thermo-sensitive genic male sterile (PTGMS) line is the basic material for two-hybrid rice and is an important genetic breeding resource. Peiai64S (PA64S) is an important germplasm resource of PTGMS rice, and it has been applied to two-line hybrid rice systems in China. Pollen fertility in PA64S is regulated by the temperature and photoperiod, but the mechanism of the fertility transition is unclear. In this study, we obtained the male fertile plant PA64S(F) and the male sterile plant PA64S(S) by controlling different temperatures under long light conditions and used the male fertile and sterile plants to investigate the role of microRNAs (miRNAs) in regulating male fertility in rice. We performed the small RNA library sequencing of anthers from PA64S(S) and PA64S(F). A total of 196 miRNAs were identified—166 known miRNAs among 27 miRNA families and 30 novel miRNAs. In the transcriptome analysis, the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of differentially expressed genes revealed significant enrichment in the synthesis and metabolism of fatty acids and some secondary metabolism pathways such as fatty acid metabolism and phenylalanine metabolism. With a comprehensive analysis of miRNA, transcriptome, and degradome sequencing, we identified that 13 pairs of miRNA/target genes regulated male fertility in rice by responding to temperature change, among which the miR156, miR5488, and miR399 affect the male fertility of PA64S by influencing SPLs, the lignin synthesis of anther walls, and the flavonoid metabolism pathway. The results provide a new understanding of PTGMS rice, which will help us better understand the potential regulatory mechanisms of male sterility in the future.

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Section: Introductionmentioning

confidence: 99%

Integrated Analysis of Small RNA, Transcriptome, and Degradome Sequencing Reveals the MiR156, MiR5488 and MiR399 Are Involved in the Regulation of Male Sterility in PTGMS Rice

Sun

Xiong

Wang

et al. 2021

IJMS

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“…Moreover, 19 and 18 known miRNAs were found to be differentially expressed in 0.5-1.0 mm buds and in 2.5-3.0 mm buds between CN12A and CN12B, respectively. Among these, 14 miRNAs were up-regulated, and 23 miRNAs were down-regulated expressed in CN12A compared with those in CN12B [27]. In this study, to identify miRNAs and their targets involved in pollen development and GMS occurrence in rapeseed, 24 small RNA libraries and transcriptome libraries were constructed and sequenced for the ower buds from the fertile and sterile lines of two RGMS lines ("6251AB" and "6284AB") and two DGMS lines ("4001AB" and "4006AB").…”

Section: Discussionmentioning

confidence: 99%

Identification of miRNAs And Their Target Genes In Genic Male Sterility Lines In Brassica Napus By Small RNA Sequencing

Jiang

et al. 2021

Preprint

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Background: Brassica napus is the third leading source of edible oil in the world. Genic male sterility (GMS) lines provide crucial material for harnessing heterosis for rapeseed. GMS lines have been used successfully for rapeseed hybrid production in China. MicroRNAs (miRNAs) play crucial regulatory roles in various plant growth, development, and stress response processes. However, reports on miRNAs that regulate the pollen development of GMS lines in B. napus are few.Results: In this study, 12 small RNA and transcriptome libraries were constructed and sequenced for the flower buds from the fertile and sterile lines of two recessive GMS (RGMS) lines, namely, “6251AB” and “6284AB”. At the same time, 12 small RNA and transcriptome libraries were also constructed and sequenced for the flower buds from the fertile and sterile lines of two dominant GMS (DGMS) lines, namely, “4001AB” and “4006AB”. Based on the results, 46 known miRNAs, 27 novel miRNAs on the other arm of known pre-miRNAs, and 44 new conserved miRNAs were identified. Thirty-five pairs of novel miRNA-3p/miRNA-5p were found. Among all the identified miRNAs, 21 differentially expressed miRNAs with over 1.5-fold change between flower buds of sterile and fertile lines were identified, including six differentially expressed miRNAs between “4001A” and “4001B”, two differentially expressed miRNAs between “4006A” and “4006B”, six differentially expressed miRNAs between “6251A” and “6251B”, and 14 differentially expressed miRNAs between “6284A” and “6284B”. The correlation analysis of small RNA and transcriptome sequencing was conducted. The analysis results indicated that 360 genes were predicted to be the candidate targets of 21 differentially expressed miRNAs. BnaA09g48720D, BnaA09g11120D, and BnaCnng51960D were demonstrated to be cleaved by bna-miR398a-3p, bna-miR158-3p and bna-miR159a, respectively. Among the differentially expressed miRNAs, miR159 was chosen to analyze its function in reproduction development and male sterility. Overexpression of bna-miR159 in Arabidopsis resulted in decreased seed setting rate, and shortened siliques, illustrating that miR159 may regulate the fertility and silique development in rapeseed.Conclusions: Our findings provide an overview of miRNAs that are potentially involved in GMS and pollen development. New information on miRNAs and their related target genes are provided to exploit the GMS mechanism and reveal the miRNA networks in B. napus.

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“…Dong et al identified 85 known miRNAs and 120 novel miRNAs, which were expressed during rapeseed anther development in a novel recessive GMS system “CN12AB.” Moreover, 19 and 18 known miRNAs were found to be differentially expressed in 0.5–1.0 mm buds and in 2.5–3.0 mm buds between CN12A and CN12B, respectively. Among these, 14 miRNAs were up-regulated, and 23 miRNAs were down-regulated expressed in CN12A compared with those in CN12B [ 27 ]. In this study, to identify miRNAs and their targets involved in pollen development and GMS occurrence in rapeseed, 24 small RNA libraries and transcriptome libraries were constructed and sequenced for the flower buds from the fertile and sterile lines of two RGMS lines (“6251AB” and “6284AB”) and two DGMS lines (“4001AB” and “4006AB”).…”

Section: Discussionmentioning

confidence: 99%

Identification of miRNAs and their target genes in genic male sterility lines in Brassica napus by small RNA sequencing

Jiang

et al. 2021

BMC Plant Biol

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Background Brassica napus is the third leading source of edible oil in the world. Genic male sterility (GMS) lines provide crucial material for harnessing heterosis for rapeseed. GMS lines have been used successfully for rapeseed hybrid production in China. MicroRNAs (miRNAs) play crucial regulatory roles in various plant growth, development, and stress response processes. However, reports on miRNAs that regulate the pollen development of GMS lines in B. napus are few. Results In this study, 12 small RNA and transcriptome libraries were constructed and sequenced for the flower buds from the fertile and sterile lines of two recessive GMS (RGMS) lines, namely, “6251AB” and “6284AB”. At the same time, 12 small RNA and transcriptome libraries were also constructed and sequenced for the flower buds from the fertile and sterile lines of two dominant GMS (DGMS) lines, namely, “4001AB” and “4006AB”. Based on the results, 46 known miRNAs, 27 novel miRNAs on the other arm of known pre-miRNAs, and 44 new conserved miRNAs were identified. Thirty-five pairs of novel miRNA-3p/miRNA-5p were found. Among all the identified miRNAs, fifteen differentially expressed miRNAs with over 1.5-fold change between flower buds of sterile and fertile lines were identified, including six differentially expressed miRNAs between “4001A” and “4001B”, two differentially expressed miRNAs between “4006A” and “4006B”, four differentially expressed miRNAs between “6251A” and “6251B”, and ten differentially expressed miRNAs between “6284A” and “6284B”. The correlation analysis of small RNA and transcriptome sequencing was conducted. And 257 candidate target genes were predicted for the 15 differentially expressed miRNAs. The results of 5′ modified RACE indicated that BnaA09g48720D, BnaA09g11120D, and BnaCnng51960D were cleaved by bna-miR398a-3p, bna-miR158-3p and bna-miR159a, respectively. Among the differentially expressed miRNAs, miR159 was chosen to analyze its function. Overexpression of bna-miR159 in Arabidopsis resulted in decreased seed setting rate, and shortened siliques, illustrating that miR159 may regulate the fertility and silique development in rapeseed. Conclusions Our findings provide an overview of miRNAs that are potentially involved in GMS and pollen development. New information on miRNAs and their related target genes are provided to exploit the GMS mechanism and reveal the miRNA networks in B. napus.

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Differentially expressed miRNAs in anthers may contribute to the fertility of a novel Brassica napus genic male sterile line CN12A

Cited by 11 publications

References 37 publications

Integrated Analysis of Small RNA, Transcriptome, and Degradome Sequencing Reveals the MiR156, MiR5488 and MiR399 Are Involved in the Regulation of Male Sterility in PTGMS Rice

Integrated Analysis of Small RNA, Transcriptome, and Degradome Sequencing Reveals the MiR156, MiR5488 and MiR399 Are Involved in the Regulation of Male Sterility in PTGMS Rice

Identification of miRNAs And Their Target Genes In Genic Male Sterility Lines In Brassica Napus By Small RNA Sequencing

Identification of miRNAs and their target genes in genic male sterility lines in Brassica napus by small RNA sequencing

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