Differentiated activities of decorin and biglycan in the progression of post-traumatic osteoarthritis

Han, Biao; Li, Q.; Wang, C.; Chandrasekaran, Prashant; Zhou, Ying; Qin, Ling; Liu, X.S.; Enomoto‐Iwamoto, Motomi; Kong, Dehan; Iozzo, Renato V.; Birk, David E.; Han, Lin

doi:10.1016/j.joca.2021.03.019

Cited by 26 publications

(13 citation statements)

References 51 publications

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“…The mice articular cartilage tissues were fixed in 4% paraformaldehyde for 48 h, embedded in paraffin, and cut into slices, which were processed and stained with alcian blue and safranin O-fast green (SO) to visualize the smoothness of cartilage surface and proteoglycan content (five sections in each group). The modified Mankin's scoring system was used to quantify the degeneration of cartilage [ 24 ].…”

Section: Methodsmentioning

confidence: 99%

Patchouli Alcohol Inhibits D-Gal Induced Oxidative Stress and Ameliorates the Quality of Aging Cartilage via Activating the Nrf2/HO-1 Pathway in Mice

Chen

Wen

Zhou

et al. 2022

Oxidative Medicine and Cellular Longevity

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Chondrocytes play an essential role in maintaining the structure and function of articular cartilage. Oxidative stress occurred in chondrocytes accelerates cell senescence and death, which contributes to the development of osteoarthritis (OA). Patchouli alcohol (PA), a kind of sesquiterpene in Pogostemon cablin, processes multiple bioactivities in treatment of many diseases. However, its effects of antisenescence and antioxidation on chondrocytes in a D-gal-induced aging mice model are still obscure. In this study, we found that PA treatment could ameliorate the degradation of cartilage extracellular matrix (ECM) in a D-gal-induced aging mice model. Further analyses through the immunofluorescent staining and western blot revealed that PA inhibited D-gal-induced chondrocyte senescence via the activation of antioxidative system. Besides, the damage caused by D-gal could not be recovered with PA treatment in Nrf2-silencing chondrocytes. In addition, molecular docking analysis between PA and Keap1 further suggested that the mechanism of PA’s antisenescence and antioxidation was attributed to the activation of Nrf2/HO-1 pathway. Therefore, our results demonstrated that PA was a promising candidate for preventing the quality loss of aging cartilage through inhibiting oxidative stress-mediated senescence in chondrocytes.

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Section: Methodsmentioning

confidence: 99%

Patchouli Alcohol Inhibits D-Gal Induced Oxidative Stress and Ameliorates the Quality of Aging Cartilage via Activating the Nrf2/HO-1 Pathway in Mice

Chen

Wen

Zhou

et al. 2022

Oxidative Medicine and Cellular Longevity

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“…Utilisation of the DCN/BGN compound (Dcn/Bgn iKO ) knockout mouse in this study indicated that following DCN loss, the accompanying loss of BGN did not aggravate OA progression. However, a significantly higher modulus was reported in the Dcn/Bgn iKO mice after meniscal destabilisation which was attributed to greater cartilage erosion [173]. The differential phenotypes following DCN or BGN ablation may be explained by differences in their localisation, binding activities and response to joint destabilisation and inflammation; furthermore, although BGN is critical in maintaining cartilage PCM integrity, its concentration and distribution is unaffected in the DMM model [171] suggesting that it does not contribute significantly to OA pathogenesis following meniscal destabilisation.…”

Section: Injurious Loadsmentioning

confidence: 85%

“…Interestingly, following PTOA induction (DMM model) in inducible BGN (Bgn iKO ) mice, the degree of OA was comparable to control mice and not as severe as the phenotype observed in the (Dcn iKO ) mice [173], implying that BGN loss has a lesser impact than DCN on DMM-induced cartilage degeneration. Utilisation of the DCN/BGN compound (Dcn/Bgn iKO ) knockout mouse in this study indicated that following DCN loss, the accompanying loss of BGN did not aggravate OA progression.…”

Section: Injurious Loadsmentioning

confidence: 94%

Mechanical Cues: Bidirectional Reciprocity in the Extracellular Matrix Drives Mechano-Signalling in Articular Cartilage

Gilbert

Bonnet

Blain

2021

IJMS

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The composition and organisation of the extracellular matrix (ECM), particularly the pericellular matrix (PCM), in articular cartilage is critical to its biomechanical functionality; the presence of proteoglycans such as aggrecan, entrapped within a type II collagen fibrillar network, confers mechanical resilience underweight-bearing. Furthermore, components of the PCM including type VI collagen, perlecan, small leucine-rich proteoglycans—decorin and biglycan—and fibronectin facilitate the transduction of both biomechanical and biochemical signals to the residing chondrocytes, thereby regulating the process of mechanotransduction in cartilage. In this review, we summarise the literature reporting on the bidirectional reciprocity of the ECM in chondrocyte mechano-signalling and articular cartilage homeostasis. Specifically, we discuss studies that have characterised the response of articular cartilage to mechanical perturbations in the local tissue environment and how the magnitude or type of loading applied elicits cellular behaviours to effect change. In vivo, including transgenic approaches, and in vitro studies have illustrated how physiological loading maintains a homeostatic balance of anabolic and catabolic activities, involving the direct engagement of many PCM molecules in orchestrating this slow but consistent turnover of the cartilage matrix. Furthermore, we document studies characterising how abnormal, non-physiological loading including excessive loading or joint trauma negatively impacts matrix molecule biosynthesis and/or organisation, affecting PCM mechanical properties and reducing the tissue’s ability to withstand load. We present compelling evidence showing that reciprocal engagement of the cells with this altered ECM environment can thus impact tissue homeostasis and, if sustained, can result in cartilage degradation and onset of osteoarthritis pathology. Enhanced dysregulation of PCM/ECM turnover is partially driven by mechanically mediated proteolytic degradation of cartilage ECM components. This generates bioactive breakdown fragments such as fibronectin, biglycan and lumican fragments, which can subsequently activate or inhibit additional signalling pathways including those involved in inflammation. Finally, we discuss how bidirectionality within the ECM is critically important in enabling the chondrocytes to synthesise and release PCM/ECM molecules, growth factors, pro-inflammatory cytokines and proteolytic enzymes, under a specified load, to influence PCM/ECM composition and mechanical properties in cartilage health and disease.

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“…Finally, images in each group were captured by using a microscopy. Moreover, the level of cartilage degeneration was also assessed and quantified by the Mankin scoring system [ 38 ]. For immunohistochemistry (IHC) staining, the sections in each group were blocked with 3% hydrogen peroxide for 10 min and 5% BSA for 30 min.…”

Section: Methodsmentioning

confidence: 99%

Curcumin primed ADMSCs derived small extracellular vesicle exert enhanced protective effects on osteoarthritis by inhibiting oxidative stress and chondrocyte apoptosis

Zhai

Ying

et al. 2022

J Nanobiotechnol

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Osteoarthritis (OA) is a common joint disease caused by progressive articular cartilage degeneration and destruction. Currently, there are no disease-modifying agents officially approved for OA patients. In this study, curcumin was loaded into adipose tissue-derived mesenchymal stem cells (ADMSCs)-derived small extracellular vesicle (ADMSCs-sEV) to synergistically exert chondro-protective effects in vitro and in vivo. We found curcumin primed ADMSCs derived sEV (sEV-CUR) exhibited an enhanced protective effect compared with free curcumin and ADMSCs-sEV in TBHP-induced chondrocytes. Moreover, our study demonstrated sEV-CUR more effectively down-regulated TBHP-induced oxidative stress and chondrocyte apoptosis in vitro. In OA mice model, our results indicated that sEV-CUR showed an improved cartilage protection, as biweekly intra-articular injection of sEV-CUR more efficaciously alleviated oxidative stress and chondrocyte apoptosis in OA cartilage. Overall, our findings showed sEV-CUR exhibited enhanced chondro-protective effects and holds great potential on the recovery of articular cartilage loss and destruction in OA patients. Graphical Abstract

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Differentiated activities of decorin and biglycan in the progression of post-traumatic osteoarthritis

Cited by 26 publications

References 51 publications

Patchouli Alcohol Inhibits D-Gal Induced Oxidative Stress and Ameliorates the Quality of Aging Cartilage via Activating the Nrf2/HO-1 Pathway in Mice

Patchouli Alcohol Inhibits D-Gal Induced Oxidative Stress and Ameliorates the Quality of Aging Cartilage via Activating the Nrf2/HO-1 Pathway in Mice

Mechanical Cues: Bidirectional Reciprocity in the Extracellular Matrix Drives Mechano-Signalling in Articular Cartilage

Curcumin primed ADMSCs derived small extracellular vesicle exert enhanced protective effects on osteoarthritis by inhibiting oxidative stress and chondrocyte apoptosis

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