Differentiating inclusion complexes from host molecules by tapping-mode atomic force microscopy

Muñoz-Botella, S.; Martin, Michel; Castillo, B. del; Vázquez, Luís

doi:10.1016/s0006-3495(96)79238-x

Cited by 13 publications

(9 citation statements)

References 15 publications

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“…53 The AFM cantilever probe applies very small-scale forces on the material while it is moving the work piece to scribe the material. 54,55 Figure 9 presents AFM images of GNIS as scanned by microscope probe in tapping mode, chosen to reduce nanocutting, 56 under ambient conditions where Figure 9a provides a cross-sectional topographic image of the sample in 2D. Unlike other characterization techniques, AFM can produce 3D images with the particular stacking and height of the nanoparticles as shown in Figure 9b, displaying the topology of the GNIS nanoparticles with the nanoscale.…”

Section: Acs Applied Materials and Interfacesmentioning

confidence: 99%

Nanopyroxene Grafting with β-Cyclodextrin Monomer for Wastewater Applications

Nafie

Vitale

Ortega

et al. 2017

ACS Appl. Mater. Interfaces

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Emerging nanoparticle technology provides opportunities for environmentally friendly wastewater treatment applications, including those in the large liquid tailings containments in the Alberta oil sands. In this study, we synthesize β-cyclodextrin grafted nanopyroxenes to offer an ecofriendly platform for the selective removal of organic compounds typically present in these types of applications. We carry out computational modeling at the micro level through molecular mechanics and molecular dynamics simulations and laboratory experiments at the macro level to understand the interactions between the synthesized nanomaterials and two-model naphthenic acid molecules (cyclopentanecarboxylic and trans-4-pentylcyclohexanecarboxylic acids) typically existing in tailing ponds. The proof-of-concept computational modeling and experiments demonstrate that monomer grafted nanopyroxene or nano-AE of the sodium iron-silicate aegirine are found to be promising candidates for the removal of polar organic compounds from wastewater, among other applications. These nano-AE offer new possibilities for treating tailing ponds generated by the oil sands industry.

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Section: Acs Applied Materials and Interfacesmentioning

confidence: 99%

Nanopyroxene Grafting with β-Cyclodextrin Monomer for Wastewater Applications

Nafie

Vitale

Ortega

et al. 2017

ACS Appl. Mater. Interfaces

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“…Dynamic AFM modes, tapping-mode AFM among them, were developed to improve lateral resolution and minimize sample distortion or damage (Martin et al, 1987;Hansma and Hoh, 1994;Putman et al, 1994;Anselmatti et al, 1994). A large variety of biomolecules, cells, and molecule-molecule interactions have been studied by tapping-mode AFM in air and liquid environments (Henderson, 1994;Fritz et al, 1995Fritz et al, , 1997Valle et al, 1996;Muñoz-Botella et al, 1996;Samori, 1998;Margeat et al, 1998). However, in most cases tapping-mode images of individual proteins are unable to reveal the fragments or subunits forming the molecule.…”

Section: Introductionmentioning

confidence: 99%

High-Resolution Imaging of Antibodies by Tapping-Mode Atomic Force Microscopy: Attractive and Repulsive Tip-Sample Interaction Regimes

Paulo

Garcia

2000

Biophysical Journal

264

197

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A force microscope operated with an amplitude modulation feedback (usually known as tapping-mode atomic force microscope) has two tip-sample interaction regimes, attractive and repulsive. We have studied the performance of those regimes to imaging single antibody molecules. The attractive interaction regime allows determination of the basic morphologies of the antibodies on the support. More importantly, this regime is able to resolve the characteristic Y-shaped domain structure of antibodies and the hinge region between domains. Imaging in the repulsive interaction regime is associated with the irreversible deformation of the molecules. This causes a significant loss in resolution and contrast. Two major physical differences distinguish the repulsive interaction regime from the attractive interaction regime: the existence of tip-sample contact and the strength of the forces involved.

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“…In imaging biological materials, TMAFM offers an important advantage over conventional contact-mode AFM: biological molecules and live cells can be imaged directly without the use of any complicated chemical methods to immobilize biological samples onto the substrate and, thus, the structure and function of biomolecules and cells can be studied at the same high level of resolution. Despite the recent success of TMAFM in imaging biological molecules and cells (Henderson, 1994; Hoh, 1994; Lal and John, 1994;Putman et al, 1994;Radmacher et al, 1995;Walivaara et al, 1995;Munoz-Botella et al, 1996;Schabert and Rabe, 1996; You and Lowe, 1996a), there are some basic aspects of TMAFM imaging of biological samples that are still not well understood, for instance, the factors determining the loading force applied to the sample, the deformation of biological samples, and most importantly, the image contrast mechanism(s). Because of the unexpected complexity of TMAFM, there have been few theoretical and experimental studies of the image contrast mechanism(s) (Putman et al, 1994;Hoper et al, 1995;Radmacher et al, 1995;Spatz et al, 1995;Chen et al, 1996;Ho and West, 1996;Howard et al, 1996;Munoz-Botella et al, 1996;Schabert and Rabe, 1996;Tamayo and Garcia, 1996).…”

Section: Introductionmentioning

confidence: 99%

“…Recently it has been reported that the frequency-dependent viscoelastic properties of the sample play an important role in determining the success and contrast of TMAFM imaging of biological samples (Putman et al, 1994;Radmacher et al, 1995;Munoz-Botella et al, 1996;Schabert and Rabe, 1996). In this work we have designed a simple system for studying the effect of the protein adsorption at the cantilever on the image contrast of TMAFM.…”

Section: Introductionmentioning

confidence: 99%

Investigation of the image contrast of tapping-mode atomic force microscopy using protein-modified cantilever tips

You

1997

Biophysical Journal

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In this work we have designed a simple system to investigate empirically the image contrast of tapping-mode atomic force microscopy (TMAFM). We modified the cantilever tips with protein molecules (bovine serum albumin or goat anti-biotin antibody) and used these protein-modified cantilevers to scan poly-L-lysine films and antibody layers deposited on mica in air under ambient conditions. We also investigated the effects of manipulating the setpoint voltage in this system. It was found that extra topographic features with a patchlike appearance were introduced into the TMAFM images of both the poly-L-lysine and antibody films when scanned with the protein-modified tips, even at initial preset setpoints, and were superimposed on the topography of the samples. The surface coverage of the patchlike features in the TMAFM images changes significantly with the setpoint voltage in a reversible and nonlinear manner. These are believed to arise from the surface indentation of the sample or from the structural deformation of the proteins at the tip induced in TMAFM imaging. Interestingly, it was observed in the experiment that no structural alteration or damage was discernible on the sample surface, even after continuous scanning with the protein-modified tips for a long period of time, with varying setpoint voltage. This study provides experimental evidence that cantilever tips modified with protein molecules or, under certain circumstances, even unmodified tips introduce extra topographical features (i.e., artifacts) and enhance the image contrast of TMAFM imaging of soft materials, which is dependent on their mechanical properties.

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Differentiating inclusion complexes from host molecules by tapping-mode atomic force microscopy

Cited by 13 publications

References 15 publications

Nanopyroxene Grafting with β-Cyclodextrin Monomer for Wastewater Applications

Nanopyroxene Grafting with β-Cyclodextrin Monomer for Wastewater Applications

High-Resolution Imaging of Antibodies by Tapping-Mode Atomic Force Microscopy: Attractive and Repulsive Tip-Sample Interaction Regimes

Investigation of the image contrast of tapping-mode atomic force microscopy using protein-modified cantilever tips

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