Differentiation and Migration of Bone Marrow Mesenchymal Stem Cells Transplanted through the Spleen in Rats with Portal Hypertension

Sun, Song; Chen, Gong; Xu, Menghua; Qiao, Yingli; Zheng, Shan

doi:10.1371/journal.pone.0083523

Cited by 28 publications

(18 citation statements)

References 42 publications

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“…Lewis antigen expression was evaluated quantitatively using the Image Pro Plus 6.0 analysis system (Media Cybernetics, Silver Spring, MD) by calculating the mean density, which was integrated optical density (IOD) divided by area of interest [ 29 ]. Three 200X fields under microscope of each slide were randomly selected and the mean density was obtained for further statistic analysis.…”

Section: Methodsmentioning

confidence: 99%

Association of Ulcerative Colitis with FUT2 and FUT3 Polymorphisms in Patients from Southeast China

Zhang

Zheng

et al. 2016

PLoS ONE

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ObjectivesDysbiosis of intestinal microbiota has been implicated in ulcerative colitis (UC). Fucosyltransferase (FUT) 2 and FUT3 determine expression of histo-blood group antigens in the gut and may affect the intestinal microbiota. We investigated the association between FUT2 and FUT3 polymorphisms and UC in Chinese patients.MethodsWe genotyped FUT2 (rs281377, rs1047781 and rs601338) and FUT3 (rs28362459, rs3745635 and rs3894326) in 485 UC patients and 580 healthy controls using SNaPshot. We also evaluated expression of Lewis a and b antigens in the sigmoid colon of 7 UC patients and 7 patients with benign colonic polyps.ResultsThe frequencies of mutant allele (A) and genotype (GA+AA) in FUT3 (rs3745635) were higher in UC patients than controls (P = 0.016, 95%CI: 1.339–1.699; P = 0.038, 95%CI: 1.330–1.742, respectively). Stratified analyses revealed that the frequencies of mutant allele (G) and genotype (TG+GG) of FUT3 (rs28362459) were significantly lower in patients with extensive colitis than those with distal colitis (P<0.001, 95%CI: 0.503–0.742; P = 0.001, 95%CI: 0.567–0.786, respectively). Similar conclusions were drawn for the mutant allele (A) and genotype (GA+AA) of FUT3 (rs3745635) in patients with extensive colitis compared to those with distal colitis (P = 0.006, 95%CI: 0.553–0.845; P = 0.011, 95%CI: 0.621–0.900, respectively). Although expression of Lewis b antigen in the sigmoid colon did not differ between UC patients and controls, Lewis a antigen expression was higher in the cryptic epithelium of both inflammatory and non-inflammatory sigmoid colon of UC patients than controls (P = 0.028).ConclusionsOur findings indicated that polymorphisms in FUT3 and its intestinal expression might be associated with UC pathogenesis.

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Section: Methodsmentioning

confidence: 99%

Association of Ulcerative Colitis with FUT2 and FUT3 Polymorphisms in Patients from Southeast China

Zhang

Zheng

et al. 2016

PLoS ONE

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“…BMSCs were isolated and cultured as previous 26 and used to examine the transgene expression. The cells were divided into three groups because of different treatment: Ad-hOPG-EGFP transduced group; Ad-EGFP transduced group and virus-free group.…”

Section: Cell Culture and Determinationmentioning

confidence: 99%

Adenoviral vector-mediated overexpression of osteoprotegerin accelerates osteointegration of titanium implants in ovariectomized rats

et al. 2015

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This study investigated the efficacy of human osteoprotegerin (hOPG) transgene to accelerate osteointegration of titanium implant in ovariectomized (OVX) rats. Bone marrow stromal cells transduced with Ad-hOPG-EGFP could sustainedly express hOPG. Osteoclast precursor RAW264.7 cells treated by the hOPG were examined by tartrate-resistant acid phosphatase (TRAP) staining and bone slice resorption assay. The results showed differentiation and function of osteoclasts were significantly suppressed by hOPG in vitro. Ad-hOPG-EGFP was locally administered to the bone defect prior to implant placement in OVX and sham rats. After 3, 7, 28 days of implantation, the femurs were harvested for molecular and histological analyses. Successful transgene expression was confirmed by western blot and cryosectioning. A significant reduction in TRAP+ numbers was detected in Ad-hOPG-EGFP group. Real-time reverse transcriptase-PCR examination revealed that hOPG transgene markedly diminished the expression of cathepsin K and receptor activator for nuclear factor-κ B ligand in vivo. The transgene hOPG modification revealed a marked increasing osteointegration and restored implant stability in OVX rats (P<0.01), compared with the control groups (Ad-EGFP or sterilized phosphate-buffered saline) 28 days after implantation. In conclusion, hOPG via direct adenovirus-mediated gene transfer could accelerate osteointegration of titanium implants in OVX rats. Osteoprotegerin gene therapy may be an effective strategy to osteointegration of implants under osteoporotic conditions.

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“…18,22,39 Different concentrations of growth factors including human hepatocyte growth factor (rhHGF), human fibroblast growth factor-4 (rhFGF-4) and EGF have been used to differentiate MSC into hepatocytes. 21,40 For assessing the differentiation of MSC to liver cells, hepatocytes marker genes such as ALB and α-fetoproteins analysed during in vitro differentiation. 5,37 ESC and MSC are two more common types of stem cells that are used to differentiate to hepatocytes.…”

Section: Rodents Models Of Human Liver Regenerationmentioning

confidence: 99%

“…To prevent bleeding and cell leakage after cell injection the lower pole of the spleen is ligated. 18,22,29,40 Tail A cell count of 5×10 5 suspended in 100µl PBS could be injected into the tail vein of rodents. 1,19,28 Portal vein Zhao and colleagues showed that intravenous injection of MSC is effective in the treatment of liver fibrosis in comparison with intrahepatic or intraperitoneal injection.…”

Section: Intrasplenicmentioning

confidence: 99%

“…Carboxy fluorescein diacetate succinimidyl ester (CFSE) 39 and 4,6-diamidino-2-phenylindole, dihydrochloride (DAPI), 24 chloromethylbenzamidodialkylcarbocyanine (CM-Dil) 40 fluorescent dyes are commonly used to examine cell localization. The red fluorochrome PKH26, which mainly binds to the cell membrane, is an efficient cell tracer to locate transplanted cells.…”

Section: Tracing Of Transplanted Cellsmentioning

confidence: 99%

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Methods of Liver Stem Cell Therapy in Rodents as Models of Human Liver Regeneration in Hepatic Failure

Goradel¹,

Darabi²,

Shamsasenjan³

et al. 2015

Adv Pharm Bull

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Differentiation and Migration of Bone Marrow Mesenchymal Stem Cells Transplanted through the Spleen in Rats with Portal Hypertension

Cited by 28 publications

References 42 publications

Association of Ulcerative Colitis with FUT2 and FUT3 Polymorphisms in Patients from Southeast China

Association of Ulcerative Colitis with FUT2 and FUT3 Polymorphisms in Patients from Southeast China

Adenoviral vector-mediated overexpression of osteoprotegerin accelerates osteointegration of titanium implants in ovariectomized rats

Methods of Liver Stem Cell Therapy in Rodents as Models of Human Liver Regeneration in Hepatic Failure

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