Differentiation and Proliferation of Pulmonary Neuroendocrine Cells

Ito, Takaaki

doi:10.1016/s0079-6336(99)80001-1

Cited by 23 publications

(16 citation statements)

References 253 publications

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“…Endocrine cells have been reported to be more abundant in fetal tissues and developing xenograft tissues than in mature xenografts, suggesting that the products of endocrine cells may be particularly important during lung ontogenesis [22]. These data are consistent with our observation that endocrine cells were rarely detectable in the present NOD/SCID-human xenograft lung system.…”

Section: Discussionsupporting

confidence: 92%

Engraftment of Adult Human Lung Tissue in Nonobese Diabetic/Severe Combined Immunodeficient Mice: A Novel Lung Epithelial Regeneration Model

et al. 2004

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Objectives: Injury causes the disruption of homeostatic cell-cell interactions and epithelial regeneration is part of the threshold response. Due to the lack of a good animal model for the investigation of these mechanisms, the kinetics of cell proliferation after injury to the human respiratory tract are poorly understood. Methods: To create a better model of human bronchioloalveolar epithelial regeneration, we engrafted adult human lung tissue into nonobese diabetic/severe combined immunodeficient (NOD/SCID) mice. Then the lung tissue was studied at various times up to 20 weeks after implantation. Results: The xenografts of bronchiolar epithelium showed characteristic features, including positivity for specific human antigens, and extensive regeneration was observed within 8 weeks after implantation. In addition, a few alveolar type II epithelial cells expressing prosurfactant protein C were detected in some areas. The distal alveolar spaces were filled with protein-rich material and were markedly dilated. Abundantly ciliated secretory epithelium, which was similar to normal adult bronchiolar epithelium, was observed within 16 weeks after implantation in the mice. All of the human lung tissue specimens that were implanted subcutaneously into the backs of the mice developed well and remained viable for 20 weeks. Each type of adult human lung epithelial cell showed a different mode of proliferation. Bronchiolar epithelial cells proliferated earlier, with MIB-1 labeling of up to 20% of the cells in the grafts at 8 weeks, while alveolar type II cells proliferated later, with labeling of up to 5% of graft cells at 12 weeks. Conclusion: This model seems to allow adult human lung epithelial regeneration to be investigated in vivo over the long term.

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Section: Discussionsupporting

confidence: 92%

Engraftment of Adult Human Lung Tissue in Nonobese Diabetic/Severe Combined Immunodeficient Mice: A Novel Lung Epithelial Regeneration Model

et al. 2004

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“…In contrast, the increase in the fraction of SP-D-immunoreactive cells peaked at day 3 and persisted beyond day 7. a) p=0.009, b) p=0.195, c) p=0.001, d) p=0.008 and e) pv0.001. performed in foetal mouse lung explants, which indicated that incubation with rHuKGF inhibits rather thanstimulates the proliferation of PNECs [37].…”

Section: Discussionmentioning

confidence: 96%

“…Experimentally induced hyperplasia of PNECs has been observed to occur in response to various toxic gases or carcinogenic stimuli [37]. Depending on the specific stimulus, the proliferative effect may be specifically restricted to a certain airway region [38,39].…”

Section: Discussionmentioning

confidence: 99%

“…Depending on the specific stimulus, the proliferative effect may be specifically restricted to a certain airway region [38,39]. PNEC proliferation was also seen in a number of non-neoplastic human lung diseases [37]. A marked increase in the number of PNECs was observed in neonatal bronchopulmonary dysplasia [40] as well as in human neonates with congenital diaphragmatic hernia (CDH) [41].…”

Section: Discussionmentioning

confidence: 99%

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Keratinocyte growth factor-induced proliferation of rat airway epithelium is restricted to Clara cellsin vivo

Fehrenbach

Fehrenbach²,

Pan³

et al. 2002

Eur Respir J

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Keratinocyte growth factor-induced proliferation of rat airway epithelium is restricted to Clara cells in vivo. H. Fehrenbach, A. Fehrenbach, T. Pan, M. Kasper, R.J. Mason. #ERS Journals Ltd 2002. ABSTRACT: Keratinocyte growth factor (KGF) is a potent mitogen of pulmonary bronchial and alveolar epithelial cells. However, it is unclear which type(s) of airway epithelial cells (AEC) proliferate(s) in response to KGF.AEC proliferation was induced in rats by either endobronchial instillation of 5 mg recombinant human (rHu) KGF per kg body weight or by adenoviral transfer of the human KGF gene (Ad5-HuKGF). Alterations in terminal airway AEC were followed for up to 7 days after rHuKGF, and for up to 28 days after Ad5-HuKGF.Cell proliferation, as assessed by immunohistochemistry (IHC) for incorporated 5-bromo-29-deoxyuridine (BrdU) and quantified by stereology, peaked at days 1-2 and was resolved by day 7 after rHuKGF and by day 21 after Ad5-HuKGF. Double immunofluorescence labelling for BrdU or Ki-67 on the one hand, and for Clara cell specific protein 10 (CC10) and calcitonin-gene related peptide on the other hand, demonstrated that Clara cells, not pulmonary neuroendocrine cells, proliferated in response to human KGF. TUNEL (terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end-labelling) method in conjunction with IHC for MNF116 failed to detect significant numbers of apoptotic AEC. IHC in conjunction with stereology revealed transient phenotypic alterations with a decrease in CC10, an increase in surfactant protein D and an increase in CD44v6 in AEC.The authors conclude that Clara cells responded to human keratinocyte growth factor in vivo by proliferation as well as by changes in protein expression, whereas no significant response was observed in pulmonary neuroendocrine cells. As Clara cells are intimately involved in airway epithelial repair, ion and fluid transport, and modulate lung inflammation, the potential of human keratinocyte growth factor to protect the lung may in part rely on the response of Clara cells.

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“…Apart from endocrine cells, which accumulate to form either distinct glands such as thyroid or isolated groups of cells within specialized organs, there is an extensive system of neuroendocrine cells that are present among other epithelial cells, especially in the airways [1][2][3]. A considerable progress has been observed recently in the knowledge on hormones produced by so-called diffuse neuroendocrine system (DNES) or the third nervous system, functioning autonomically and regulating the function of all internal organs [4][5][6].…”

Section: Introductionmentioning

confidence: 99%

Quantitative characteristics of calcitonin-producing cells in the thyroid and lungs of uremic rats.

Kasacka

2009

Folia Histochem Cytobiol.

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Uremia leads to a number of metabolic and hormonal disorders induced by renal failure with definite biological and clinical sequels. For this reason and the absence of reports on influence of CRF on calcitonin (CT)-producing cells of the thyroid glands and airways, the author decided to investigate the behavior of neuroendocrine cells in experimental uremia, taking CT-producing cells as an example. The aim of the present study was to examine the number and distribution of CT-producing cells in the thyroid glands and lungs of uremic rats. Fragments of the thyroids and lungs were collected one week after nephrectomy. Paraffin-embedded sections were stained with H+E and by silver impregnation. To identify neuroendocrine cells, immunohistochemical reaction was performed with the use of a specific antibody against calcitonin. It was revealed that the number of CT-immunoreactive cells decrease in the thyroid and considerable increase in the lungs of rats, when compared to the value in the control animals. The results can be regarded as the morphological manifestation of calcitonin-producing endocrine cells in the rat thyroid and lungs to disorders in the internal environment of the body induced by the impairment of renal parenchyma functioning.

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Differentiation and Proliferation of Pulmonary Neuroendocrine Cells

Cited by 23 publications

References 253 publications

Engraftment of Adult Human Lung Tissue in Nonobese Diabetic/Severe Combined Immunodeficient Mice: A Novel Lung Epithelial Regeneration Model

Engraftment of Adult Human Lung Tissue in Nonobese Diabetic/Severe Combined Immunodeficient Mice: A Novel Lung Epithelial Regeneration Model

Keratinocyte growth factor-induced proliferation of rat airway epithelium is restricted to Clara cellsin vivo

Quantitative characteristics of calcitonin-producing cells in the thyroid and lungs of uremic rats.

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